P62 sirna

Manufactured by Cell Signaling Technology

Sourced in United States

P62 siRNA is a small interfering RNA (siRNA) targeting the gene encoding the P62 protein. P62, also known as SQSTM1, is a multifunctional protein involved in various cellular processes, including autophagy and signal transduction. The P62 siRNA can be used to study the function of the P62 protein in biological systems.

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Lab products found in correlation

4 protocols using p62 sirna

Autophagy Detection and Modulation

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Cyto-ID autophagy detection kit was obtained from Enzo Life Sciences (Villeurbanne, France). Live and dead cell assay kit was purchased from Abcam (Cambridge, UK). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), bafilomycin A1 (BaF A1), chloroquine (CQ), 3-methyladenine (3-MA) formononectin, decursin and N-acetyl-L-cysteine (NAC) were from Sigma-Aldrich (St. Louis, MO, USA). Alexa fluor-488, Alexa fluor-594 and Lipofectamine reagent were obtained from Invitrogen (Garlsbad, CA, USA). Protein A/G plus-agarose beads and p62 siRNA were purchased cell signaling (Danvers, MA, USA). pBABE-puromCherry-EGFP-LC3B (addgene 22418) and EF.STAT3C.Ubc.GFP (addgene 24983) were from addgene (Cambridge, MA, USA). Nodakenin was purchased from ChemFaces (Wuhan, China) and cucurbitacin D was obtained from Extrasynthese (Genay, France).

Choi Y.K., Cho S.G., Choi Y.J., Yun Y.J., Lee K.M., Lee K., Yoo H.H., Shin Y.C, & Ko S.G. (2016). SH003 suppresses breast cancer growth by accumulating p62 in autolysosomes. Oncotarget, 8(51), 88386-88400.

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Investigating Apoptosis and Autophagy Regulation in Cellular Responses

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Fetal bovine serum (FBS), RPMI-1640, and penicillin-streptomycin were purchased from HyClone (Victoria, Australia). 3-(4,5-dimethyl-thiazol-2-yl)-2, 5-diphenyltetra-zolium bromide (MTT) and JC-1 fluorescent dye (Sigma-Aldrich, St Louis, MO), LY294002 and SP600125 (selleck, Texas, Houston), Protein A/G plus Agarose immunoprecipitation reagent (Santa Cruz, CA), DAPI (C0060, Solarbio, Beijing, China), lysis buffer (Beyotime, Shanghai, China), Annexin- V/FITC Apoptosis Detection Kit (BestBio, Shanghai, China). Antibodies specific for β-actin (sc-1615), Bax (sc-493), Bcl-2 (sc-783), Nrf2 (sc-13,032), c-Jun (sc-74,543) and p-c-Jun (sc-53,182) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). p-Nrf2 (ab76026), p62 (ab56416), Anti-active caspase 3 (ab2302) and anti-active caspase 9 (ab2324) were purchased from Abcam (Cambridge, MA). SAPK/JNK siRNA (#6232), p62 siRNA (#6394), Poly (ADP-ribose) polymerase-1 (PARP-1) (#9532), Cleaved PARP (#5625), NQO1 (#3187), Keap1 (#4617), Bcl-xL (#2764), XIAP (#14334), JNK (#9252), p-JNK (#9255), LC3B (#3868S) and Autophagy antibody sampler kit (#4445) including Beclin-1, ATG16, ATG3, ATG7 and ATG5 were purchased from Cell Signaling Technology (Danvers, MA). The enhanced chemiluminescence (ECL) kit was purchased from ThermoFisher (Waltham, MA).

Yu H., Wu C.L., Wang X., Ban Q., Quan C., Liu M., Dong H., Li J., Kim G.Y., Choi Y.H., Wang Z, & Jin C.Y. (2019). SP600125 enhances C-2-induced cell death by the switch from autophagy to apoptosis in bladder cancer cells. Journal of Experimental & Clinical Cancer Research : CR, 38, 448.

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Alzheimer's Disease Pathways Modulation

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Amyloid-β 1-42 (GenicBio, A-42-T-1), Rapamycin (Sigma Aldrich, R0395), Bafilomycin A1 (Sigma Aldrich, B1793), Latrunculin B (Sigma Aldrich, L5288), Jasplakinolide (AdipoGen Life Sciences, 102396-24-7), p62 siRNA (Cell Signaling Technology, 6399S), SSH1 siRNA (Dharmacon GE Healthcare, 5′-GAG GAG CUG UCC CGA UGA C-3′), Cofilin siRNA (Dharmacon GE Healthcare, 5′-GGA GGA CCU GGU GUU CAU C-3′).

Cazzaro S., Fang C., Khan H., Witas R., Kee T.R., Woo J.A, & Kang D.E. (2022). Slingshot homolog-1 mediates the secretion of small extracellular vesicles containing misfolded proteins by regulating autophagy cargo receptors and actin dynamics. Frontiers in Aging Neuroscience, 14, 933979.

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Regulation of NLRP3 Inflammasome by Autophagy

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ox-LDLs were purchased from Yiyuanbiotech (Guangzhou, China). Phorbol-12-myristate-13-acetate (PMA), rapamycin, 3-methyladenine (3-MA), bafilomycin A1 and protease inhibitor cocktails were purchased from MCE (Monmouth Junction, USA). LPS and ATP were purchased from Sigma (St Louis, MO, USA). Lipofectamine™ 2000 was purchased from Invitrogen (Carlsbad, USA). Antibody to GAPDH, antibody to β-actin and HRP-conjugated secondary polyclonal antibodies to mouse and rabbit IgGs were purchased from Elabscience (Wuhan, China). Rabbit monoclonal anti-NLRP3, anti-ASC, anti-SQSTM1/p62, anti-LC3B, antibody to K63-linked polyubiquitin chains, antibody to K48-linked polyubiquitin chains and p62-siRNA, and control siRNA were purchased from Cell Signaling Technology (Boston, USA). Rabbit monoclonal anti-IL-1β, anti-caspase-1 were purchased from Abcam (Cambridge, MA, USA). For the immunoprecipitation, polyclonal rabbit anti-SQSTM1/p62 was purchased from Abcam (Cambridge, MA, USA). Monoclonal mouse anti-SQSTM1/p62, anti-NLRP3, anti-ASC and protein A/G Plus-agarose were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). AMSH (the JAMM/MPN+ family of K63-specific DUB) was purchased from Boston Biochem (Cambridge, MA, USA).

Zhou Z., Zhu X., Yin R., Liu T., Yang S., Zhou L., Pan X, & Ma A. (2020). K63 ubiquitin chains target NLRP3 inflammasome for autophagic degradation in ox-LDL-stimulated THP-1 macrophages. Aging (Albany NY), 12(2), 1747-1759.

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