Confocal microscope

Manufactured by Zeiss

Sourced in Germany, United States, Japan, United Kingdom, China

The Zeiss Confocal Microscope is an optical imaging technique that captures high-resolution, three-dimensional images by scanning the sample with a focused laser beam. It allows for the efficient elimination of out-of-focus light, resulting in sharper, more detailed images compared to conventional optical microscopes.

Automatically generated - may contain errors

Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (28 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (23 mentions) Triton x 100, by Merck Group (16 mentions) Bovine serum albumin (bsa), by Merck Group (15 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (14 mentions) Zen blue software, by Zeiss (12 mentions) Alexa fluor 488, by Thermo Fisher Scientific (11 mentions) Vectashield, by Vector Laboratories (10 mentions) 4 6 diamidino 2 phenylindole (dapi), by Solarbio (9 mentions) Zen software, by Zeiss (9 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (9 mentions) Paraformaldehyde (pfa), by Merck Group (8 mentions) Propidium iodide, by Thermo Fisher Scientific (6 mentions) Fluorescence microscope, by Leica (5 mentions) Lsm software, by Zeiss (5 mentions) Goat serum, by Merck Group (5 mentions) Ab150077, by Abcam (5 mentions) Facscalibur, by BD (5 mentions) Hoechst 33342, by Thermo Fisher Scientific (5 mentions) Mounting medium, by Agilent Technologies (4 mentions)

Close spelling variants of this product

710 meta upright confocal microscopes LSM710 confocal laser-scanning microscopes LSM980 confocal microscopes Axio-Imager_LSM-800 confocal microscope Axio Observer Z1 confocal microscope Multiphoton confocal laser-scanning microscope LSM 710 FCS confocal microscope Zeiss7 DUO NLO confocal laser microscope LSM900 confocal laser microscope Carl Zeiss LSM780 confocal microscope

860 protocols using confocal microscope

Immunofluorescence and TUNEL Assay in ARPE-19 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Phosphate buffered saline (PBS)-washed, paraformaldehyde-fixated (4%, 20 min; Merck, Darmstadt, Germany) ARPE-19 cells were permeabilized (PBS/0.2% Tween20 (PBS-T), 45 min), and unspecific bindings were blocked (3% bovine serum albumin (BSA) (Carl Roth, Karlsruhe, Germany)/PBS-T, 1 h). Antigens were detected using a primary antibody (Supplementary Materials, Table S1, overnight, 3% BSA/PBS-T) and a fluorescence-conjugated antispecies antibody (Supplementary Materials, Table S1, 45 min, 3% BSA/PBS). The fluorochrome HOECHST 33342 (1:1000) was used to stain DNA. Cells were covered with fluorescence mounting medium (Dako, Agilent Technologies, Santa Clara, CA, USA). Images were taken with a confocal microscope (Zeiss, Jena, Germany).
The TUNEL assay was performed with a DeadEnd™ Fluorometric TUNEL System (Promega, Madison, WI, USA) on paraformaldehyde-fixated, washed, and permeabilized (0.2% Triton X-100 in PBS) cells. Images were taken with a confocal microscope (Zeiss, Jena, Germany).

Trakkides T.O., Schäfer N., Reichenthaler M., Kühn K., Brandwijk R.J., Toonen E.J., Urban F., Wegener J., Enzmann V, & Pauly D. (2019). Oxidative Stress Increases Endogenous Complement-Dependent Inflammatory and Angiogenic Responses in Retinal Pigment Epithelial Cells Independently of Exogenous Complement Sources. Antioxidants, 8(11), 548.

+ Open protocol

+ Expand

Chondrogenic Differentiation of hUC-MSCs by LDH

Check if the same lab product or an alternative is used in the 5 most similar protocols

hUC-MSCs were cultured with different LDH (MgAl–Cl or MgFe–NO₃) for 14 days. Then, the cells in each group were fixed with 4% PFA (Sigma-Aldrich, St. Louis, MO, USA), permeabilized with 0.25% Triton X-100 (Amresco, Cleveland, OH, USA), blocked with 5% donkey serum (Jackson ImmunoResearch, West Grove, PA, USA), and primary SOX9 antibody (Abcam, Cambridge, MA, USA) was incubated at 4 °C overnight. The next day, the samples were conjugated with a fluorescent secondary antibody (Invitrogen, Carlsbad, CA, USA) at room temperature and nuclei were stained with 4ʹ,6-diamidino-2-phenylindole (DAPI; Abcam). The expression of key genes during chondrogenic differentiation was observed and photographed using a confocal microscope (Carl Zeiss). The excitation wavelength of DAPI was 405 nm and that of SOX9 was 488 nm.
For the pathway inhibition study, hUC-MSCs were cultured with MgFe–NO₃ LDH, Ac-PHSCN-NH2 (ATN; Selleck, TX, USA), and LDH + ATN for 7 days. Immunofluorescence staining was performed after 7 days of culture. The staining steps were the same as above, and the primary antibody to integrin, was purchased from Abcam. Fluorescence was observed using a confocal microscope (Carl Zeiss). The excitation wavelengths were 405 nm for DAPI, 488 nm for SOX9, 647 nm for integrin, and 594 nm for F-actin.

Wang Z., Yang H., Xu X., Hu H., Bai Y., Hai J., Cheng L, & Zhu R. (2022). Ion elemental-optimized layered double hydroxide nanoparticles promote chondrogenic differentiation and intervertebral disc regeneration of mesenchymal stem cells through focal adhesion signaling pathway. Bioactive Materials, 22, 75-90.

+ Open protocol

+ Expand

Visualizing Porous Hydrogel Structures

Check if the same lab product or an alternative is used in the 5 most similar protocols

In order to visualize porous hydrogel structures, untreated (not freeze-dried after polymerization) and freeze-dried hydrogels were incubated with 200 µM YFP-LecB, washed twice with PBS, cut into small fragments and analysed at the Zeiss Confocal Microscope (Carl Zeiss Ag, Oberkochen, Germany) at 488 nm (Fig. 3). To investigate YFP-LecB specificity for the hydrogels, the YFP-LecB gels were diffused by 200 µM GFP for 1 min and analysed at the Zeiss Confocal Microscope (Carl Zeiss AG, Oberkochen, Germany) at 488 and 514 nm.

Bodenberger N., Kubiczek D., Trösch L., Gawanbacht A., Wilhelm S., Tielker D, & Rosenau F. (2017). Lectin-mediated reversible immobilization of human cells into a glycosylated macroporous protein hydrogel as a cell culture matrix. Scientific Reports, 7, 6151.

+ Open protocol

+ Expand

Live/Dead Staining of S. aureus Biofilm

Check if the same lab product or an alternative is used in the 5 most similar protocols

The live dead staining of bacterial cells was done using standard protocol,^{29 (link)} with variations. Briefly, the glass cover slip with pre-formed S. aureus biofilm was washed extensively with double distilled water, followed by washing with PBS, pH 7.0. The staining for viable cells on the cover slips was performed by the addition and 5 μM of Syto9 (ThermoFischer Scientific, USA), diluted in DMSO. The staining was done for 10 min in dark conditions. After incubation, the cover slips were further washed extensively with 1× PBS and final rinsing in double distilled water. In order to stain for nonviable S. aureus cells, propidium iodide (ThermoFischer Scientific, USA) was diluted in 2× SSC buffer (0.3 M NaCl, 0.03 M sodium citrate, pH 7.0) to a final concentration of 500 nm. The diluted propidium iodide was added to the washed and rinsed Syto9 stained cover slip. The staining was done for 10 min, followed by washing with 1× PBS and final rinsing with double distilled water. The imaging was done by Confocal microscope (Zeiss, Germany) at an excitation/emission of 483/503 nm for Syto9 and 535/617 nm for propidium iodide. The images were acquired on a Rolera Em-C² camera with a 63× oil immersion objective (Zeiss, Germany). The acquired image was processed by Zen software (Zeiss, Germany).

Manoharadas S., Altaf M., Alrefaei A.F., Devasia R.M., Badjah Hadj A.Y, & Abuhasil M.S. (2021). Concerted dispersion of Staphylococcus aureus biofilm by bacteriophage and ‘green synthesized’ silver nanoparticles. RSC Advances, 11(3), 1420-1429.

+ Open protocol

+ Expand

Immunohistochemical and Immunofluorescent Analysis of Follistatin in Liver Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Paraffin-embedded liver sections were deparaffinized, hydrated, thermally repaired with 0.01 M sodium citrate buffer, and then incubated in 3% hydrogen peroxide for 10 min to eliminate endogenous peroxidase activity. Next, the sections were incubated with 5% BSA for 1 h at 37°C and primary antibody against FST (#60060-1-Ig, Proteintech, China) at 4°C overnight and then washed with phosphate-buffered saline three times.
For IHC staining, the sections were incubated with secondary antibody and StreptAvidin—Biotin Complex (SABC) for 30 min each (#SA1050, BOSTER, China), and then 3.3’-diaminobenzidine (DAB; #AR1022, BOSTER, China) was added for 3 min. Finally, hematoxylin staining was performed for cell counting. The IHC results were based on 10 random fields in each section using an optical microscope (Zeiss, Germany).
For IF staining, fluorescent secondary antibody (Alexa Fluor^® 488-labeled goat anti-mouse IgG(H+L); #ab150113, Abcam) was added for 1 h at room temperature, and then the cell nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) for 5 min. The IF images were obtained using a confocal microscope (Zeiss, Germany).

Tong J., Cong L., Jia Y., He B.L., Guo Y., He J., Li D., Zou B, & Li J. (2022). Follistatin Alleviates Hepatic Steatosis in NAFLD via the mTOR Dependent Pathway. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 15, 3285-3301.

+ Open protocol

+ Expand

Immunostaining of Drosophila Neuronal Tissues

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies used in this work include Guinea pig anti Oc (1:750, gift from Tiffany Cook) (Xie et al., 2007 (link)); Rabbit anti Sox102F and Rabbit anti Slp1 (1:500 for both, Gifts from Claude Desplan); Rabbit anti-D (1:200) (from John R. Nambu), mouse anti-Ey (1:10, DSHB), sheep anti-GFP (1:500, AbD Serotec), Goat anti anti-beta-gal (Abcam 1:1000), rabbit anti-RFP (Abcam 1:1000), Rabbit anti HA (Cell Signaling Technology, 1:1000). Secondary antibodies are from Jackson or Invitrogen. Immunostaining was done as described (Li et al., 2013 (link)) with a few modifications: 3^rd instar Larval brains or adult brains were dissected in 1XPBS, and fixed in 4% Formaldehyde for 30 minutes on ice (larval) or 45min at RT (adult). Brains were incubated in primary antibody solution overnight at 4°C, washed three times and incubated in secondary antibody solution overnight at 4°C, washed three times and mounted in Slowfade. Images are acquired using a Zeiss Confocal Microscope. Figures are assembled using Photoshop and Illustrator.

Naidu V.G., Zhang Y., Lowe S., Ray A., Zhu H, & Li X. (2020). Temporal progression of Drosophila medulla neuroblasts generates the transcription factor combination to control T1 neuron morphogenesis. Developmental biology, 464(1), 35-44.

+ Open protocol

+ Expand

Mitochondrial Stress Protein Localization in Chondrocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

For determination of localization of mitochondrial stress proteins in chondrocytes, a modified Mito-Tracker Red CMXRos kit (Beyotime, China) was used according to manufacturer’s instructions to label mitochondria, at an excitation of 579 nm and an emission of 599 nm. Cultured cells were washed thrice with PBS and fixed in 4% paraformaldehyde for 20 min. Cells were first treated with CMXRos for 20 min. The cells were permeabilized for 10 min with 0.1% Triton X-100 and blocked with 5% BSA for 30 min at room temperature. Next, the cells were stained with anti-HSP10, anti-ClpP, or anti-LONP1 antibodies (1:500; Bioss, Beijing, China) with 1% BSA overnight at 4 °C. The next day, the cells were washed three times with PBS for 5 min, incubated with the corresponding fluorescent-labeled secondary antibody (FITC, 1:200 Boster, Wuhan) for 1 h in the dark, washed three times with PBS for 5 min, and counterstained with 4′,6-diamidino-2-phenylindole (DAPI) for 5 min. Images were captured using a confocal microscope (Zeiss, Germany). FITC was analyzed with excitation at 495 nm and emission at 591 nm. DAPI was analyzed with excitation at 353 nm and emission at 465 nm.

Zai Z., Xu Y., Qian X., Li Z., Ou Z., Zhang T., Wang L., Ling Y., Peng X., Zhang Y, & Chen F. (2022). Estrogen antagonizes ASIC1a-induced chondrocyte mitochondrial stress in rheumatoid arthritis. Journal of Translational Medicine, 20, 561.

+ Open protocol

+ Expand

Spatio-temporal analysis of cell fate

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fertilized chicken eggs were incubated at 37 °C until Hamburger and Hamilton stage 18/19. A small window was made in the eggshell, followed by a hole in the upper membrane through which 200 µL of 200 mM FUrd or 50 mM EdU was injected. Eggs were resealed with tape and incubated at 37 °C for 1 h. Embryos were fixed in 4% paraformaldehyde (PFA) for 1 h at room temperature, washed in PBS then incubated overnight in 30% sucrose and subsequently frozen down in Tissue-Tek O.C.T. (Sakura) for sectioning. Sections were washed in PBS and stained overnight at 4 °C for FUrd and Snail1/2, see (Supplementary Table 1) for antibody details. Secondary antibodies listed previously were applied at 1:1000 dilution for 45 min, followed by DAPI stain. EdU detection was achieved using Click-IT azide-Alexa Fluor 488 (Life Technologies) according to the manufacturer’s protocol. All images were captured with a Zeiss Confocal microscope. Chick experiments were repeated at least 3 times with 2 or more embryos.

Prakash V., Carson B.B., Feenstra J.M., Dass R.A., Sekyrova P., Hoshino A., Petersen J., Guo Y., Parks M.M., Kurylo C.M., Batchelder J.E., Haller K., Hashimoto A., Rundqivst H., Condeelis J.S., Allis C.D., Drygin D., Nieto M.A., Andäng M., Percipalle P., Bergh J., Adameyko I., Farrants A.K., Hartman J., Lyden D., Pietras K., Blanchard S.C, & Vincent C.T. (2019). Ribosome biogenesis during cell cycle arrest fuels EMT in development and disease. Nature Communications, 10, 2110.

+ Open protocol

+ Expand

RNA FISH Assay for CircRNA Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Briefly, T24 and SW780 cells were seeded on cell slides, fixed with 4% paraformaldehyde, treated with 0.5% Triton and prehybridized. Overnight hybridization was then performed with probes (10 mM). The assays were conducted using RNA FISH kit (Guangzhou RiboBio Co., Ltd., Guangzhou, Guangdong, China) according to the manufacturer’s instructions. The Cy3-labeled circ_0000629 probe was synthesized and provided by Sangon Biotech (Shanghai, China). Images were taken with a confocal microscope (Carl Zeiss, Oberkochen, Germany).

Wang J., Luo J., Wu X, & Gao Z. (2021). Circular RNA_0000629 Suppresses Bladder Cancer Progression Mediating MicroRNA-1290/CDC73. Cancer Management and Research, 13, 2701-2715.

+ Open protocol

+ Expand

Immunocytochemistry for DUSP6 Localization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were seeded into 6-well plates pre-coated with poly-L-lysine (Sigma-Aldrich Chemical, St. Louis, MO, USA) and stabilized for 24 h. After fixation with 4% of formaldehyde (Sigma-Aldrich Chemical, St. Louis, MO, USA) for 10 min, cells were treated with 0.25% TritonX-100 (Sigma-Aldrich Chemical, St. Louis, MO, USA) to permeabilize all lipid bilayers for 10 min. After being washed with PBS buffer two times, cells were blocked with 1% of BSA solution at room temperature for 30 min. Primary antibody against DUSP6 was added to cells at 4 °C overnight. Then, cells were incubated with mouse anti-rabbit IgG-CFL 488 for 1 h. Nuclei of cells were stained using DAPI. Fluorescent images were measured by confocal microscope (Carl Zeiss, Oberkochen, Germany) and quantified by ImageJ version 1.53n (National Institutes of Health, Bethesda, MD, USA).

Kim S., Kim M, & Sung J.S. (2022). Exposure of Toluene Diisocyanate Induces DUSP6 and p53 through Activation of TRPA1 Receptor. International Journal of Molecular Sciences, 23(1), 517.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!