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Taqman polymerase chain reaction assay

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The TaqMan polymerase chain reaction (PCR) assay is a laboratory technique used for detecting and amplifying specific DNA sequences. It utilizes fluorescent probes to monitor the amplification of target DNA in real-time during the PCR process.

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Lab products found in correlation

Automated biochemical technique, by Siemens (4 mentions) Type i4000, by Abbott (3 mentions) Lightcycler 480 real time pcr system, by Roche (3 mentions) Abi 7500 real time pcr system, by Thermo Fisher Scientific (2 mentions) Architect i4000sr system, by Abbott (2 mentions) Advia 2120 hematology analyzer, by Siemens (1 mentions)

5 protocols using taqman polymerase chain reaction assay

1

Hepatitis B Biomarker Quantification

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Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatitis B surface antigen (HBsAg), hepatitis B surface antibody (HBsAb), HBeAg, hepatitis B e antibody (HBeAb), hepatitis B core antibody (HBcAb), HBV DNA and alpha-fetoprotein (AFP) were quantified as described in a previous study[24 (link)]. Briefly, ALT and AST were determined using an automated biochemical system (Siemens Healthcare Diagnostics, USA). Serum HBsAg, HBsAb, HBeAg, HBeAb and HBcAb were measured using the automatic immune fluorescence analyser Abbott Type i4000 (Abbott Laboratories, USA), following the manufacturer’s instructions. Serum HBV DNA was quantified using the TaqMan polymerase chain reaction (PCR) assay (Sansure Biotech, China) in a Roche LightCycler 480 Real-Time PCR System (Roche, Switzerland) with a low quantification limit of 500 IU/ml.
Fu Y., Fang F., Guo H., Xiao X., Hu Y., Zeng Y., Chen T., Wu S., Lin N., Huang J., Jiang L., Ou Q, & Liu C. (2022). Compartmentalisation of Hepatitis B virus X gene evolution in hepatocellular carcinoma microenvironment and the genotype-phenotype correlation of tumorigenicity in HBV-related patients with hepatocellular carcinoma. Emerging Microbes & Infections, 11(1), 2486-2501.
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2

Routine Biochemical and Serological Analysis of Hepatitis B

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Routine biochemical tests including total protein (TP), albumin (Alb), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBil), direct bilirubin (DB), and indirect bilirubin (IDB) were detected by automated biochemical technique (Siemens Healthcare Diagnostics, USA). Serum HBV DNA was quantified using the TaqMan polymerase chain reaction (PCR) assay (Sansure Biotech, China) on ABI 7500 Real‐Time PCR System (Life Technologies, USA), which has a detection limit of 20 IU/mL. HBsAg, HBsAb, HBeAg, HBeAb, and HBcAb were measured using a commercial chemiluminescent microparticle immunoassay kit with the Architect i4000SR System (Abbott Laboratories, USA).
Zeng Y., Wu W., Fu Y., Chen S., Chen T., Yang B, & Ou Q. (2019). Toll‐like receptors, long non‐coding RNA NEAT1, and RIG‐I expression are associated with HBeAg‐positive chronic hepatitis B patients in the active phase. Journal of Clinical Laboratory Analysis, 33(5), e22886.
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3

Hepatitis B Serological and Molecular Markers

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Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined by automated biochemical technique (Siemens Healthcare Diagnostics, USA) with cutoff value of 40 IU/L. Serum HBsAg, anti-HBs, hepatitis B e antigen (HBeAg), anti-HBe and anti-HBc were measured on the wholly automatic immune fluorescence analyzer Abbott Type i4000 (Abbott Laboratories, USA) using the original, corollary commercial kits. Serum HBV DNA was quantified using the TaqMan polymerase chain reaction (PCR) assay (Sansure Biotech, China) on Roche LightCycler 480 Real-Time PCR System (Roche, Switzerland) with a low limit of quantification of 500 IU/ml.
Fu Y., Wu S., Hu Y., Chen T., Zeng Y., Liu C, & Ou Q. (2020). Mutational characterization of HBV reverse transcriptase gene and the genotype-phenotype correlation of antiviral resistance among Chinese chronic hepatitis B patients. Emerging Microbes & Infections, 9(1), 2381-2393.
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4

Hepatitis B Biomarker Quantification

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Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined by automated biochemical technique (Siemens Healthcare Diagnostics). Serum HBsAg, anti‐HBs, hepatitis B e antigen (HBeAg), anti‐HBe, and anti‐HBc were measured on the wholly automatic immune fluorescence analyzer Abbott Type i4000 (Abbott Laboratories) using the original, corollary commercial kits. Serum HBV DNA was quantified using the TaqMan polymerase chain reaction (PCR) assay (Sansure Biotech) on Roche LightCycler 480 Real‐Time PCR System (Roche) with a low limit of quantification of 500 IU/ml.
Lai X., Chen W., Wu Y., Gao Y., Zhang Y., Xu X., Fu Y., Wang X., Yang Y, & Zhang Y. (2022). Effect of mutations across reverse transcriptase region on HBV replication and progression of liver diseases in Chinese patients. Journal of Clinical Laboratory Analysis, 36(7), e24530.
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5

Comprehensive Biomarker Assessment for HBV

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Blood samples were collected at every visit time. Routine blood testing was performed on Siemens ADVIA 2120 Hematology Analyzer. Routine biochemical tests including alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT), etc. were measured by automated biochemical technique (Siemens Healthcare Diagnostics, USA). Serum HBV DNA was quantified using the TaqMan polymerase chain reaction (PCR) assay (Sansure Biotech, China) on ABI7500 Real‐Time PCR System (Life Technologies, USA), which has a detection limit of 500 IU/mL. The levels of HBsAg, HBsAb, HBeAg, HBeAb and HBcAb were measured using a commercial chemiluminescent microparticle immunoassay kit with the Architect i4000SR System (Abbott Laboratories, USA).
Lin S., Fu Y., Wu W., Chen T., Chen N., Xun Z., Liu C., Ou Q., Zeng Y, & Huang H. (2020). The efficacy of addition of Tenofovir Disoproxil Fumarate to Peg-IFNα-2b is superior to the addition of Entecavir in HBeAg positive CHB patients with a poor response after 12 weeks of Peg-IFNα-2b treatment alone. International Journal of Medical Sciences, 17(10), 1458-1463.
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