Anhydrous calcium chloride cacl2

Anti-A20, anti-elastin antibody was obtained from Santa Cruz Technology (Santa Cruz Biotech, Santa Cruz, CA, USA). Primary polyclonal antibodies against phospho-IKKβ (Ser180/181), phospho-IκBα (Ser32/36), IKKβ, IκBα, and NF-κB p65 are from Cell Signaling Technology. Anti-CD45, anti-CD68 and anti-CD20 were from Boster, China. Anti-β-actin, anti-MMP-2, anti-MMP-9 were purchased from Bioss, China. Calcium chloride anhydrous (CaCl₂) and zinc sulfate heptahydrate (ZnSO₄·7H₂O) were purchased from Sigma. Diaminobenzidine (DAB) and strept-avidin biotin complex (SABC) immunohistochemical kit were purchased from Boster (Wuhan, China). Fetal bovine serum and Dulbecco's modified Eagle's medium (DMEM) were purchased from Hyclone (Logan, Utah, USA).

In order to fabricate composite granules, the following reagents were used: sodium alginate (Sigma Aldrich, US), chondroitin sulphate sodium salt (TCI, Belgium), calcium chloride anhydrous CaCl₂ (Sigma-Aldrich, China) and the following previously synthesized powders and microgranules: HA, Ag-HA, Ga-HA, AgM and GaM.
At first, a 4% aqueous sodium alginate solution was prepared at 40 °C and chondroitin sulphate sodium salt was added to obtain a 0.5% suspension. Then, two types of composite granules were prepared.
In the first type of granules, 1 g of Ag-HA or Ga-HA (or HA) powder was added to the suspension (10 mL) and mixed vigorously, resulting in a milky, dense slurry. Meanwhile, the cross-linking solution (1.5% CaCl₂) was prepared. Finally, the slurry was added dropwise to a CaCl₂ solution, stirred using a magnetic stirrer and granules were formed. The granules obtained were left in the cross-linking agent for 10 min, rinsed with distilled water, dried in air and then lyophilized.
During the preparation of the second type of granules, pure, unsubstituted HA and AgM or GaM microgranules (ratio 1:1) were used instead of Ag-HA or Ga-HA powders. The other stages of production remained unchanged. All the obtained granules are listed in Table 6.

Zwitterionic starch (AL-Z) was synthesized following the methodology reported in the previous article [26 (link)]. Polyethylene glycol (PEG, Mw~1000) was purchased from Merck KGaA (Darmstadt, Germany). Polycaprolactone diol (PCL-diol, Mw~2000), isophorone diisocyanate (IPDI), N, N-Dimethylformamide (anhydrous, 99.8%) (DMF), and potato starch (soluble) (AL-N) were purchased from Sigma-Aldrich (St. Louis, MO, USA). A Micro BCA™ Protein Assay Kit and Triton^® X-100 were obtained from Thermo Scientific (Waltham, MA, USA). A CytoTox 96^® Non-Radioactive Cytotoxicity Assay kit was provided by Promega (Woods Hollow Road, Madison, WI, USA) and a Human Thrombin-Antithrombin Complex ELISA Kit (TAT) by Abcam (Cambridge, UK). Sodium dodecyl sulfate (SDS), ethanol absolute, calcium chloride anhydrous (CaCl₂), and bovine albumin serum (BSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). A 0.9% sodium chloride solution was acquired from Baxter (Deerfield, Illinois, USA). Phosphate buffer solution (PBS) was obtained from VWR^® (Radnor, PA, USA). Human umbilical vein endothelial cells (HUVECs), human aortic smooth muscle cells (AoSMC), an EGM BulletKit, and an SmGM-2 BulletKit were purchased from Lonza (Basel, Switzerland).