1 methyl 4 phenyl 1 2 3 6 tetrahydropyridine mptp

First, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 1-methyl-4-phenylpyridinium (MPP⁺) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tyrosine hydroxylase (TH) was purchased from Merck Millipore (Bedford, MA, USA). Brain-derived neurotrophic factor (BDNF), cyclooxygenase-2 (COX-2), B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), cytochrome c, mitochondrial transcription factor A (TFAM), and nuclear factor kappa B-p65 (NF-kB p65), were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Phosphorylated cyclic AMP response element binding (pCREB), cyclic-AMP response element binding (CREB), sirtuin-1 (SIRT-1), peroxisome proliferator-activated receptor-γ coactivator 1-α (PGC-1α), cleaved caspase3, inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNF-α), and voltage-dependent anion channel (VDAC), were purchased from Cell Signaling Technology (Beverly, MA, USA). β-actin purchased from Abcam (Cambridge, MA, USA) and all other chemicals used in this study were of analytical grade.

All antibodies used in Western blotting were obtained from Cell Signaling Technology (Beverly, MA, USA) except histidine antibody (Santa Cruz Biotechnology; Santa Cruz, CA, USA). PEP-1-GLRX1 protein was purified as previously described [27 (link),33 (link)]. PEP-1 peptides were purchased from PEPTRON (Daejeon, Korea). Methyl-4-phenylpyridinium (MPP⁺) and 1-methyl-4-phenyl-1,2,3,6-tetrahydro pyridine (MPTP) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Human neuroblastoma cells (SH-SY5Y) were cultured in MEM with 15% FBS, 2 mM L-glutamine, and 100 µg/mL gentamicin at 37 °C.

Human umbilical cords were obtained from healthy women who had childbirth in our hospital. All samples obtained were provided by these women who agreed and signed consent forms. C57BL mice (6–8 weeks) were ordered from Beijing Vital River Laboratory Animal Technology Co. Collection of samples and handling animals were all approved by the Ethics Committee of Neurology Department of General Hospital of Jinan Military Region. All experiments were reviewed by the Ethics Committee of Neurology Department of General Hospital of Jinan Military Region. Adenovirus expressing green fluorescent protein gene (Ad-GFP) was provided by Beckman Medical Instruments, USA. Recombinant adenovirus carrying FGF-20 gene (Ad-FGF-20) was constructed in our lab.
Consumables such as F12 culture medium, pipettes, and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) were all ordered from Sigma (USA). Fetal calf serum was ordered from HyClone Company (USA). All ELISA reagent kits were ordered from IBL (Germany). All antibodies were ordered from Abcam (England). Chromogenic substrate kit was ordered from Shanghai Ruichi Biotechnology Co., Ltd. (Shanghai, China).