C18 4 3 mm i d security guard cartridge

High performance liquid chromatography (HPLC) were used to quantify DON, T-2 and HT-2 from media. In addition, liquid chromatography tandem mass spectrometry (LC-MS/MS) were used to quantify FUMS from media and Fusarium toxins from grains. HPLC used consisted of an Agilent 1200 Series system equipped with a UV diode array detector (DAD) set at 220.4 nm (Agilent Technologies, Palo Alto, CA, USA). The column used for the chromatographic separation was a Phenomenex^® Gemini C₁₈, 150 mm × 4.6 mm, 3 µm (Phenomenex, Macclesfield, UK) preceded by a Phenomenex^® Gemini 3 mm guard cartridge and the column temperature was set at 25 °C. LC-MS/MS used consisted of an QTrap 5500 LC-MS/MS System (Applied Biosystems, Foster City, CA, USA) equipped with a TurboIonSpray electrospray ionization (ESI) source and an 1290 Series HPLC System (Agilent, Waldbronn, Germany). Chromatographic separation was performed at 25 °C on a Gemini^® C₁₈-column, 150 × 4.6 mm i.d., 5 µm particle size, equipped with a C₁₈ 4 × 3 mm i.d. security guard cartridge (all from Phenomenex, Torrance, CA, USA).

The analysis of sterigmatocystin was performed using an HPLC‐MS/MS method for the determination of 186 fungal and bacterial metabolites described by Vishwanath et al. (2009). Detection and quantification in the Selected Reaction Monitoring (SRM) mode was performed with a QTrap 4000 LC‐MS/MS System (Applied Biosystems, Foster City, CA, USA) equipped with a TurboIonSpray electrospray ionization (ESI) (Applied Biosystems, Foster City, CA, USA) source and an 1100 Series HPLC System (Agilent, Waldbronn, Germany). Chromatographic separation was performed at 25°C on a Gemini C18 column, 150  ×  4.6‐mm i.d., 5 μm particle size, equipped with a C₁₈ 4 × 3 mm‐i.d. security guard cartridge (Phenomenex, Torrance, CA, USA). Injection volume was 5 μl. For quantification, external calibration was performed using multi‐analyte standards prepared and diluted in a 1:1 mixture of extraction and dilution solvent.
All chemicals (LC gradient grade) were from J.T. Baker (Deventer, The Netherlands); ammonium acetate (MS grade) and glacial acetic acid (p.a.) were obtained from Sigma‐Aldrich (Vienna, Austria). Water was purified successively by reverse osmosis and a Milli‐Q plus system from Millipore (Molsheim, France). A certified standard for sterigmatocystin in acetonitrile was from Biopure Referenzsubstanzen GmbH (Tulln, Austria) and Sigma (Vienna, Austria).

The analysis at the SEM was carried out as previously described^{26 (link)}. LC-MS/MS screening of target fungal metabolites was performed with a QTrap 5500 LC-MS/MS System (Applied Biosystems, Foster City, CA) equipped with a TurboIonSpray electrospray ionization (ESI) source and an 1290 Series HPLC System (Agilent, Waldbronn, Germany). Chromatographic separation was performed at 25 °C on a Gemini^® C18-column, 150 × 4.6 mm i.d., 5 µm particle size, equipped with a C18 4 × 3 mm i.d. security guard cartridge (all from Phenomenex, Torrance, CA, US). The chromatographic method, as well as the chromatographic and mass spectrometric parameters, have been previously described^{30 (link)}, but the method has in the meantime been expanded to cover 650 metabolites (manuscript in preparation).