Taqman primer probe mixes

Manufactured by Integrated DNA Technologies

TaqMan primer/probe mixes are pre-designed, ready-to-use oligonucleotides for real-time PCR assays. These mixes contain the necessary primers and probes to perform target-specific gene expression analysis or detection.

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Lab products found in correlation

Trizol, by Thermo Fisher Scientific (2 mentions) Taqman gene expression master mix, by Thermo Fisher Scientific (2 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

TaqMan primer/probe mix Primer/probe mix TaqMan primers TaqMan probes

2 protocols using taqman primer probe mixes

Quantitative RT-PCR Analysis of Gene Expression

Cited 1 time

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RNA was isolated using TRIzol (Life Technologies). Conversion to cDNA and subsequent qRT-PCR analysis were performed using the High-Capacity cDNA Reverse Transcription Kit and Taqman Gene expression Master Mix (Applied Biosystems). Predesigned Taqman primer/probe mixes were purchased from Integrated DNA Technologies. Data are presented as fold changes in RNA levels compared to control treatment, calculated following the 2^−ΔΔCt method, with GAPDH and Actin Beta serving as two internal control genes [17 (link), 18 (link)].

Yoshida K., Taga T., Saito M., Suematsu S., Kumanogoh A., Tanaka T., Fujiwara H., Hirata M., Yamagami T., Nakahata T., Hirabayashi T., Yoneda Y., Tanaka K., Wang W.Z., Mori C., Shiota K., Yoshida N, & Kishimoto T. (1996). Targeted disruption of gp130, a common signal transducer for the interleukin 6 family of cytokines, leads to myocardial and hematological disorders.. Proceedings of the National Academy of Sciences of the United States of America, 93(1), 407-411.

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Quantitative RT-PCR Gene Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA isolated using TRIzol (Life Technologies) was reverse transcribed to cDNA using the high-capacity cDNA reverse transcription kit (Applied Biosystems) for subsequent qRT-PCR analysis. Taqman primer/probe mixes predesigned by Integrated DNA Technologies and Taqman gene expression master mix (Applied Biosystems) was used to perform qRT-PCR. Data are presented as fold changes in RNA levels compared to control treatment, calculated following the 2^−ΔΔCt method.

, & Mead C.A. (1997). Collective electrodynamics I. Proceedings of the National Academy of Sciences of the United States of America, 94(12), 6013-6018.

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