Log In Sign up
The largest database of trusted experimental protocols

Anti htt epr5526

Manufactured by Abcam
Visit Supplier

Anti-HTT EPR5526 is a recombinant rabbit monoclonal antibody that recognizes the Huntingtin (HTT) protein. The antibody was generated using Abcam's proprietary Exponential Amplification Technology (ExT).

Automatically generated - may contain errors

Lab products found in correlation

Hdac1, by Abcam (2 mentions) Anti atp5a, by Abcam (2 mentions) Anti darpp32, by Abcam (2 mentions) Anti polyq php3, by Abcam (2 mentions) Anti pde10a, by Abcam (2 mentions) Anti vinculin, by Merck Group (2 mentions) Anti gapdh, by Merck Group (2 mentions) Anti gfap, by Merck Group (2 mentions) Anti polyq mw1, by Merck Group (2 mentions) Anti htt mw8, by Developmental Studies Hybridoma Bank (2 mentions) Odyssey clx imaging system, by LI COR (1 mentions) Anti htt d7f7, by Cell Signaling Technology (1 mentions)

3 protocols using anti htt epr5526

1

SDS-PAGE and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
SDS-PAGE and western blot analysis were performed according to standard protocols. Primary antibodies used in western blots are anti-HTT EPR5526 (Abcam), anti-HTT D7F7 (Cell Signaling Technologies) and anti-Flag #F4799 (Sigma). Secondary antibodies used in western blots are goat-anti-rabbit IgG-IR800 (LI-COR) and donkey anti-mouse IgG-IR680 (LI-COR). Membranes were visualised on an Odyssey® CLx Imaging System (LI-COR).
Harding R.J., Deme J.C., Hevler J.F., Tamara S., Lemak A., Cantle J.P., Szewczyk M.M., Begeja N., Goss S., Zuo X., Loppnau P., Seitova A., Hutchinson A., Fan L., Truant R., Schapira M., Carroll J.B., Heck A.J., Lea S.M, & Arrowsmith C.H. (2021). Huntingtin structure is orchestrated by HAP40 and shows a polyglutamine expansion-specific interaction with exon 1. Communications Biology, 4, 1374.
+ Open protocol
+ Expand
2

Antibodies and Dilutions for Huntington's Disease

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies and dilutions were used in this study: Anti-HTT Ab1 (aa1–17,1 (link)) 1:50 for capillary immunoassay and 1:2000 for western blot; anti-HTT EPR5526 (Abcam, Waltham, MA, ab109115, 1:2000 for western blot); anti-polyQ MW1 (MilliporeSigma, Burlington, MA, MABN2427, 1:50 for capillary immunoassay); anti-polyQ PHP3 (generous gift from Dr. Ali Khoshnan, 1:2000 for western blot); Anti-PDE10A (Abcam, Waltham, MA, #ab177933, 1:2000 for western blot); Anti-DARPP32 (Abcam, #ab40801, 1:2000 for capillary immunoassay); Anti-GFAP (MilliporeSigma, Burlington, MA, AB5804, 1:3000 for capillary immunoassay); Anti-GAPDH (MilliporeSigma, Burlington, MA, #MAB374, 1:10000 for western blot); Anti-Sodium channel subunit beta-4 (Abcam, Waltham, MA, #ab80539, 1:500 for western blot); Anti-vinculin (Sigma, St. Louis, MO, #V9131, 1:5000 for capillary immunoassay, 1:2000 for western blot); Anti-ATP5A (Abcam, Waltham, MA, #ab14748, 1:2000 for western blot); Anti-HTT MW8 (University of Iowa Developmental Studies Hybridoma Bank, 1:1000 for filter trap); Anti-HTT S830 (generous gift from Dr. Gillian Bates, 61 (link) 1:8000), HDAC1 (Abcam, Waltham, MA, ab32369–7, 1:4000).
Shing K., Sapp E., Boudi A., Liu S., Seeley C., Marchionini D., DiFiglia M, & Kegel-Gleason K.B. (2023). Early whole-body mutant huntingtin lowering averts changes in proteins and lipids important for synapse function and white matter maintenance in the LacQ140 mouse model. bioRxiv.
+ Open protocol
+ Expand
3

Huntington's Disease Protein Immunoanalysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies and dilutions were used in this study: Anti-HTT Ab1 (aa1–17,(Difiglia et al., 1995 (link))) 1:50 for capillary immunoassay and 1:2000 for western blot; anti-HTT EPR5526 (Abcam, Waltham, MA, ab109115, 1:2000 for western blot); anti-polyQ MW1 (MilliporeSigma, Burlington, MA, MABN2427, 1:50 for capillary immunoassay); anti-polyQ PHP3 (generous gift from Dr. Ali Khoshnan, 1:2000 for western blot); Anti-PDE10A (Abcam, Waltham, MA, #ab177933, 1:2000 for western blot); Anti-DARPP32 (Abcam, #ab40801, 1:2000 for capillary immunoassay); Anti-GFAP (MilliporeSigma, Burlington, MA, AB5804, 1:3000 for capillary immunoassay); Anti-GAPDH (MilliporeSigma, Burlington, MA, #MAB374, 1:10000 for western blot); Anti-Sodium channel subunit beta-4 (Abcam, Waltham, MA, #ab80539, 1:500 for western blot); Anti-vinculin (Sigma, St. Louis, MO, #V9131, 1:5000 for capillary immunoassay, 1:2000 for western blot); Anti-ATP5A (Abcam, Waltham, MA, #ab14748, 1:2000 for western blot); Anti-HTT MW8 (University of Iowa Developmental Studies Hybridoma Bank, 1:1000 for filter trap); Anti-HTT S830 (generous gift from Dr. Gillian Bates, (Landles et al., 2010 (link)) 1:8000), HDAC1 (Abcam, Waltham, MA, ab32369–7, 1:4000).
Shing K., Sapp E., Boudi A., Liu S., Seeley C., Marchionini D., DiFiglia M, & Kegel-Gleason K.B. (2023). Early whole-body mutant huntingtin lowering averts changes in proteins and lipids important for synapse function and white matter maintenance in the LacQ140 mouse model. Neurobiology of disease, 187, 106313.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!