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Embryomax nucleosides

Manufactured by Merck Group
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EmbryoMax nucleosides are a specialized laboratory product manufactured by Merck Group. They are synthetic compounds designed for use in cell culture and embryological research applications. The core function of EmbryoMax nucleosides is to provide essential nutrients and building blocks for the growth and development of cells, tissues, and embryos in controlled laboratory environments.

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Lab products found in correlation

Non essential amino acids (neaa), by Thermo Fisher Scientific (6 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (5 mentions) B27 supplement, by Thermo Fisher Scientific (4 mentions) Glutamax, by Thermo Fisher Scientific (4 mentions) Mem non essential amino acid, by Thermo Fisher Scientific (4 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Activin a, by Thermo Fisher Scientific (3 mentions) Ovotransferrin, by Merck Group (3 mentions) Newborn calf serum, by Thermo Fisher Scientific (2 mentions) Sodium pyruvate, by Thermo Fisher Scientific (2 mentions) β mercaptoethanol, by Merck Group (2 mentions) Antibiotic antimycotic, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Biowest (2 mentions) Esgro complete plus clonal grade medium, by Merck Group (2 mentions) Esgro mlif, by Merck Group (2 mentions) Coulter single cell, by Beckman Coulter (2 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Dexamethasone (dex), by Merck Group (2 mentions) Heparin sodium, by Merck Group (2 mentions)

Close spelling variants of this product

Nucleosides (64 citations) EmbryoMax (31 citations) EmbryoMax ES Cell Qualified Nucleosides (2 citations)

28 protocols using embryomax nucleosides

1

Maintaining Transgenic Mouse ESCs

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Transgenic and RW4 mouse ESCs were cultured on gelatin-coated T-25 flasks in complete media, consisting of Dulbecco’s Modified Eagle Medium (DMEM; Life Technologies #11965-092, Carlsbad, CA) containing 10% newborn calf serum (Life Technologies #16010-159), 10% fetal bovine serum (Life Technologies #26140-079), and 1x Embryomax Nucleosides (Millipore #ES-008-D, San Francisco, CA). ESCs were passaged every two days at a 1:5 ratio in fresh complete media containing 1000 U/mL leukemia inhibitory factor (LIF; Millipore # ESG1106) and 100μM β-mercaptoethanol (BME; Life Technologies #21985-023).
Iyer N.R., Huettner J.E., Butts J.C., Brown C.R, & Sakiyama-Elbert S.E. (2016). Generation of Highly Enriched V2a Interneurons from Mouse Embryonic Stem Cells. Experimental neurology, 277, 305-316.
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2

Cultivation of Chicken Embryonic Stem Cells

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The OSCs were cultured in a 12‐well glass bottom plate (NEST Biotechnology) pre‐seeded with irradiated chicken embryo fibroblasts feeder. The culture medium is KnockOut‐Dulbecco's Modified Eagle Medium (KO‐DMEM) (Gibco) containing 6 ng/ml basic fibroblast growth factor (bFGF) (Solarbio), 25 ng/ml Human Activin A (Novoprotein), 7.5% defined fetal bovine serum (Hyclone), 2.5% chicken serum (Solarbio), 1 × antibiotic‐antimycotic (Gibco), 1 × GlutaMAX (Gibco), 1 × NEAA (Gibco), 1 × B‐27 Supplement (Gibco), 1 × EmbryoMax Nucleosides (Millipore), 1.2 mM Sodium Pyruvate (Gibco), and 0.1 mM β‐mercaptoethanol (Sigma‐Aldrich). Cells were cultured at 39°C in 5% CO2, with fresh medium replaced every other day, then they were subcultured every 3–5 days upon confluence. The PGCs were isolated from the E5.5 gonad according to a previous protocol21 and maintained with the same conditions of OSCs.
Meng L., Zhang Y., Hua Y., Ma Y., Wang H., Li X., Jiang Y, & Zhu G. (2022). Identification of oogonial stem cells in chicken ovary. Cell Proliferation, 56(3), e13371.
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3

Culturing Mouse Embryonic Stem Cells

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J1, CJ7, and E14TG2a (E14) male mouse ESCs were cultured on 0.1% gelatin-coated plates in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 18% fetal bovine serum (BioWest), MEM nonessential amino acids (Gibco), EmbryoMax nucleosides (Millipore), 50 U/mL penicillin/streptomycin/L-glutamine (PSG, Gibco), 100 µM β-mercaptoethanol, and 1000 U/mL recombinant mouse leukemia inhibitory factor (LIF, Millipore) in a 37°C with 5% CO2. Media was changed daily, and cells were passaged every 2 days. J1, CJ7, E14TG2a mouse ES cells and HEK293T cells were all obtained from ATCC (except CJ7 line was obtained from Dr. Stuart Orkin), confirmed by partial genomic DNA sequencing. Mycoplasma contamination was not detected by PCR based methods.
LeBlanc L., Lee B.K., Yu A.C., Kim M., Kambhampati A.V., Dupont S.M., Seruggia D., Ryu B.U., Orkin S.H, & Kim J. (2018). Yap1 safeguards mouse embryonic stem cells from excessive apoptosis during differentiation. eLife, 7, e40167.
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4

Fructose Rescue for GLUT3-Deficient Cells

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CRC cells were cultured in glucose-free DMEM with 10% dFBS, 6 mM fructose and 100 μM nucleosides (EmbryoMax nucleosides: Millipore) to observe the rescue efficacy for cells with GLUT3 deletion and attenuated glucose utilization.
Dai W., Xu Y., Mo S., Li Q., Yu J., Wang R., Ma Y., Ni Y., Xiang W., Han L., Zhang L., Cai S., Qin J., Chen W.L., Jia W, & Cai G. (2020). GLUT3 induced by AMPK/CREB1 axis is key for withstanding energy stress and augments the efficacy of current colorectal cancer therapies. Signal Transduction and Targeted Therapy, 5, 177.
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5

Murine Embryonic Stem Cell Culture

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J1, CJ7, and E14TG2a (E14) male mESCs were cultured on tissue culture-treated plates coated in 0.1% gelatin with +LIF medium, which is composed of Dulbecco's Modified Eagle's Medium (DMEM, Gibco) supplemented with 18% fetal bovine serum (BioWest), EmbryoMax nucleosides (Millipore), MEM nonessential amino acids (Gibco), 100 μM β-mercaptoethanol, 50 U/mL penicillin/streptomycin/L-glutamine (PSG, Gibco), as well as 1000 U/mL recombinant mouse LIF (Millipore) in a 37°C incubator with 5% CO2. Cells were passaged every other day and media was changed daily. For cell culture experiments, cells were routinely seeded at 1.9×105 cells/cm2 for high density (HD) and 2.3×104 cells/cm2 for low density (LD). For differentiation, cells were passaged on d0 to transfer into -LIF medium (same as +LIF, except without LIF) and seeded on d1 at the appropriate densities (high or low). J1 and E14 mESCs and HEK293T cells were from ATCC, whereas CJ7 mESCs were obtained from Dr. Stuart Orkin. PCR did not detect mycoplasma contamination. For serum-free culture, mESCs were maintained in NDiff 227 (Takara Bio) supplemented with 1 μM PD0325901 (Selleck Chemicals), 3 μM CHIR99021 (Selleck Chemicals), and 1000 U/mL recombinant mouse LIF (2i media) and differentiated by withdrawal of LIF and both inhibitors.
LeBlanc L., Kim M., Kambhampati A., Son A.J., Ramirez N, & Kim J. (2021). β-catenin links cell seeding density to global gene expression during mouse embryonic stem cell differentiation. iScience, 25(1), 103541.
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6

Mouse ESC Maintenance and Culture

Cited 2 times
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Mouse ESCs (E14Tg2a; ATCC, CRL-1821) were maintained on gelatin (Sigma, G1890)-coated plates in the ESGRO complete plus clonal grade medium (Millipore), as previously described24 (link),60 (link). For experiments, ESCs were cultured on gelatin-coated plates in the M15 medium: DMEM (Thermo Fisher, 11965084) supplemented with 15% FBS (Gemini, 100–125), 10 μM 2-mercaptoethanol (Sigma, M3148), 0.1 mM nonessential amino acids (Thermo Fisher, 11140050), 1x EmbryoMax nucleosides (Millipore, ES-008-D), 1 U/ml of ESGRO mLIF (Millipore, ESG1107).
Oldfield A.J., Henriques T., Kumar D., Burkholder A.B., Cinghu S., Paulet D., Bennett B.D., Yang P., Scruggs B.S., Lavender C.A., Rivals E., Adelman K, & Jothi R. (2019). NF-Y controls fidelity of transcription initiation at gene promoters through maintenance of the nucleosome-depleted region. Nature Communications, 10, 3072.
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7

Proliferation of Cells with Nucleosides

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Cells were seeded at a density of 50000–100000 cells in duplicates. For one set of wells nucleosides were added (EmbryoMax nucleosides: Millipore) and incubated for 72 hours. Every other day, the media was replenished and nucleosides were added. The media was aspirated and then the cells were washed with PBS, trypsinized, spun down and resuspended in media and counted using a Beckman coulter single cell.
Kollareddy M., Dimitrova E., Vallabhaneni K.C., Chan A., Le T., Chauhan K.M., Carrero Z.I., Ramakrishnan G., Watabe K., Haupt Y., Haupt S., Pochampally R., Boss G.R., Romero D.G., Radu C.G, & Martinez L.A. (2015). Regulation of Nucleotide Metabolism by Mutant p53 Contributes to its Gain-of-Function Activities. Nature communications, 6, 7389.
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8

Maintenance and Differentiation of Mouse Embryonic Stem Cells

Cited 2 times
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J1 ESCs were obtained from the American Type Culture Collection. ZHBTc4 cells are kindly provided by Dr. Hitoshi Niwa. They were cultured on gelatin-coated plates in the ESGRO complete plus clonal grade medium (for maintenance) or the M15 medium (for experiments) as described before (Zheng et al., 2012 (link)). M15 medium contains DMEM supplemented with 15% FBS, 10 μM 2-mercaptoethanol, 1 mM nonessential amino acids, 1 × EmbryoMax nucleosides, 1000 U/ml of LIF (Millipore). ESC differentiation and transfections were carried out similarly as described previously (Wang et al., 2013 (link)).
Wang L., Du Y., Ward J.M., Shimbo T., Lackford B., Zheng X., Miao Y.L., Zhou B., Han L., Fargo D.C., Jothi R., Williams C.J., Wade P.A, & Hu G. (2014). INO80 Dependent Promoter Access Facilitates Activation of Pluripotency Genes in Embryonic Stem Cell Self-Renewal, Reprogramming, and Blastocyst Development. Cell stem cell, 14(5), 575-591.
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9

Cell Cycle Arrest and DNA Damage Response

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Where indicated, cells were treated with 2 mM hydroxyurea, 2.5 μm aphidicolin, 10 μm etoposide, 10 μm 5-fluorouracil, 1 μM cisplatin, 1 μM gemcitabine, 0.001 % methyl methanesulfonate, 100 nM doxorubicin, 3 μM camptothecin, 300 μM nucleosides, 10 nM afatinib, 30 nM lapatinib, 150 nM rapamycin, 15 μm MEK inhibitor U0126, 150 nM AKT inhibitor MK2206, 7.5 μm PI3K inhibitor LY294002, 1 μm ATR kinase inhibitor VE821 (AdooQ), CCT244747 (a kind gift from Prof. Ian Collins, ICR, London), 10 μm ATM kinase inhibitor KU55933 (Merck, Millipore), 100 nM UCN01 Chk1/PKCβ inhibitor (Merck, Millipore), 12.5–300 μM EmbryoMax Nucleosides (Millipore).
Kanu N., Cerone M.A., Goh G., Zalmas L.P., Bartkova J., Dietzen M., McGranahan N., Rogers R., Law E.K., Gromova I., Kschischo M., Walton M.I., Rossanese O.W., Bartek J., Harris R.S., Venkatesan S, & Swanton C. (2016). DNA replication stress mediates APOBEC3 family mutagenesis in breast cancer. Genome Biology, 17, 185.
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10

Culturing Mouse Embryonic Stem Cells

Cited 2 times
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Mouse ESCs (E14Tg2a, ATCC) were maintained on gelatin-coated plates in the ESGRO complete plus clonal grade medium (Millipore), as previously described (Cinghu et al., 2014 (link); Oldfield et al., 2014 (link)). For experiments, ESCs were cultured on gelatin-coated plates in M15 medium: DMEM (Invitrogen) supplemented with 15% FBS, 10μM 2-mercaptoethanol, 0.1 mM nonessential amino acids (Invitrogen), 1× EmbryoMax nucleosides (Millipore), and 1U/ml of ESGRO mLIF (Millipore). Dicer KO mouse ESCs (Novus Biologicals) and Exosc3 KO mouse ESCs, which was a kind gift from U. Basu (University of Columbia) (Pefanis et al., 2015 (link)), were grown on inactivated MEFs (Gibco) using the M15 medium. All cells used in the study were routinely tested for mycoplasma contamination.
Cinghu S., Yang P., Kosak J.P., Conway A.E., Kumar D., Oldfield A.J., Adelman K, & Jothi R. (2017). Intragenic enhancers attenuate host gene expression. Molecular cell, 68(1), 104-117.e6.
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