Gastric cancer cell lines, OCUM-2M (Yashiro et al, 1995 (link)), OCUM-2MD3 (Yashiro et al, 1996 (link)), OCUM-12 (Kato et al, 2010 (link)), KATO-III, and NUGC-3 (Nomura et al, 2001 (link)), derived from diffuse-type gastric carcinomas, and MKN-74(Motoyama et al, 1986 (link)), derived from non-diffuse-type gastric carcinoma, were used. The culture medium consisted of DMEM (Wako, Osaka, Japan) with the addition of 10% fetal bovine serum (FBS; Nichirei, Tokyo, Japan), 100 IU ml−1 penicillin (Wako), 100 mg ml−1 streptomycin (Wako), and 0.5 mM sodium pyruvate (Wako). Cells were cultured at 37 °C in 21% O2.
Sodium pyruvate
Manufactured by Fujifilm
Sourced in Japan, United States
Sodium pyruvate is a chemical compound that is commonly used in cell culture media and laboratory settings. It is a salt of pyruvic acid, which is an important intermediate in cellular metabolism. Sodium pyruvate serves as an energy source for cells and can be used to supplement cell culture media to support cell growth and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
L glutamine, by Fujifilm (23 mentions)
Penicillin, by Fujifilm (13 mentions)
Streptomycin, by Fujifilm (12 mentions)
Phenol red, by Fujifilm (12 mentions)
Penicillin streptomycin, by Fujifilm (11 mentions)
Dulbecco modified eagle medium (dmem), by Fujifilm (10 mentions)
Non essential amino acids (neaa), by Fujifilm (10 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (9 mentions)
2 mercaptoethanol, by Fujifilm (8 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions)
Fetal bovine serum (fbs), by Nichirei Biosciences (6 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (6 mentions)
Mem non essential amino acids, by Fujifilm (5 mentions)
Fetal bovine serum (fbs), by Biowest (5 mentions)
Glutamax, by Thermo Fisher Scientific (5 mentions)
Hepes, by Fujifilm (4 mentions)
L glutamine, by Nacalai Tesque (4 mentions)
2 mercaptoethanol, by Thermo Fisher Scientific (4 mentions)
Fetal bovine serum (fbs), by Merck Group (4 mentions)
Mem non essential amino acids solution, by Fujifilm (4 mentions)
70 protocols using sodium pyruvate
1
Gastric Cancer Cell Line Cultivation
2
Mesenchymal Stem Cell Differentiation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For MSC differentiation, a 70% subconfluent culture of MSCs from the third passage were used.
The MSCs were placed in basic medium, consisting of Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen Life Technologies), 10% fetal bovine serum (FBS), 1% penicillin, 1% streptomycin and 1% amphotericin B. Specific supplements were added to this basic medium formulation for the differentiation of the MSCs into various mesenchymal lineages[28 (link)]. Adipogenic differentiation was induced with 0.5 μM dexamethasone, 0.5 Mm 3-isobutyl-1-methylxanthine and 0.1 mM indomethacin (Sigma)[29 (link)]. Osteogenic differentiation was achieved with 0.1 μM of dexamethasone, 50 μM ascorbic acid and 10 mM-glycerophosphate (Sigma)[30 (link)]. Chondrogenic differentiation was induced with 50 μM ascorbic acid, 0.1 μM dexamethasone, 10 ng/ml transforming growth factor-beta (TGF-β) (R&D Systems), 40 μg/ml L-proline (Sigma- Aldrich) and 100 μg/ml sodium pyruvate (Wako) [31 (link)]. The differentiation medium was refreshed every 2 days. Differentiation into adipocytes, osteocytes and chondrocytes was confirmed by oil red O, von Kossa and toluidine blue staining, respectively[30 (link)]
The MSCs were placed in basic medium, consisting of Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen Life Technologies), 10% fetal bovine serum (FBS), 1% penicillin, 1% streptomycin and 1% amphotericin B. Specific supplements were added to this basic medium formulation for the differentiation of the MSCs into various mesenchymal lineages[28 (link)]. Adipogenic differentiation was induced with 0.5 μM dexamethasone, 0.5 Mm 3-isobutyl-1-methylxanthine and 0.1 mM indomethacin (Sigma)[29 (link)]. Osteogenic differentiation was achieved with 0.1 μM of dexamethasone, 50 μM ascorbic acid and 10 mM-glycerophosphate (Sigma)[30 (link)]. Chondrogenic differentiation was induced with 50 μM ascorbic acid, 0.1 μM dexamethasone, 10 ng/ml transforming growth factor-beta (TGF-β) (R&D Systems), 40 μg/ml L-proline (Sigma- Aldrich) and 100 μg/ml sodium pyruvate (Wako) [31 (link)]. The differentiation medium was refreshed every 2 days. Differentiation into adipocytes, osteocytes and chondrocytes was confirmed by oil red O, von Kossa and toluidine blue staining, respectively[30 (link)]
3
Gastric Cancer Cell Lines Under Hypoxia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Eight gastric cancer cell lines, OCUM12,3 OCUM‐12/hypo,3 OCUM‐2MD3,11 OCUM‐2MD3/hypo,11 NUGC‐3,12 NUGC‐3/hypo, NUGC‐4,12 and NUGC‐4/hypo, were used in this study. NUGC‐3 and NUGC‐4 cells were obtained from the JCRB cell bank (Osaka, Japan). Four hypoxia‐resistant cell lines, OCUM‐12/hypo, OCUM‐2MD3/hypo, NUGC‐3/hypo cells, and NUGC‐4/hypo, were established from the parent cell lines, OCUM12, OCUM‐2MD3, NUGC‐3, and NUGC‐4, respectively, by continuous exposure to 1% oxygen up to 5% oxygen for 4 weeks, as previously reported.3 The culture condition was cultivated in DMEM (Nikken, Kyoto, Japan) with the addition of 10% FBS (Nichirei, Tokyo, Japan), 100 IU/mL penicillin (Wako, Osaka, Japan), 100 mg/mL streptomycin (Wako), and 0.5 mM sodium pyruvate (Wako) at 37°C. All experimental studies by parent cancer cells were carried out at 20% O2, and those by hypoxia‐resistant cells were carried out at 1% O2.
4
Reagents and Antibodies for Cell Culture Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dulbecco’s modified Eagle’s medium (DMEM) was obtained from Nissui Pharmaceutical (Tokyo, Japan). The following reagents were used: foetal bovine serum (FBS) (Biosera, Kansas City, MO, USA)), l -glutamine (Thermo Fisher Scientific, Waltham, MA, USA), penicillin, sodium pyruvate (Wako Pure Chemical Industries, Osaka, Japan), non-essential amino acids (Thermo Fisher Scientific), G418 (Nacalai Tesque, Kyoto, Japan), and blasticidin (Kaken Pharmaceutical, Kyoto, Japan). Monoclonal antibodies against FLNA (EMD Millipore, Billerica, MA, USA), glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Cell Signalling Technology, Danvers, MA, USA), and FLAG M2 (Sigma-Aldrich, St. Louis, MO, USA); polyclonal antibodies against FLNB (EMD Millipore) and FLNC (Atlas Antibodies, Stockholm, Sweden); and horseradish peroxidase-conjugated anti-rabbit and -mouse IgG (Cell Signalling Technology) were used.
5
Culturing Nalm-6 Pre-B Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Nalm-6 cells were cultured in a 5% CO2 incubator at 37 °C in MEM medium (Nissui Seiyaku) supplemented with 10% calf serum (Hyclone), 2 mM MEM non-essential amino acids (Wako Pure Chemical), 1 mM sodium pyruvate (Wako Pure Chemical), 50 μM 2-mercaptoethanol (Wako Pure Chemical) and 0.15 μM Vitamin B12 (Sigma-Aldrich). Nalm-6 is a human pre-B cell line available from ATCC and was kindly provided by Dr Michael R. Lieber. The Nalm-6 cell line was originally established from the peripheral blood of a 19-year-old man with acute lymphoblastic leukaemia59 (link) and has a stable near-diploid karyotype60 (link). All cell lines used in this study are not listed in the database of commonly misidentified cell lines maintained by ICLAC and were tested for mycoplasma contamination using an e-Myco Mycoplasma PCR Detection Kit (iNtRON Biotechnology, Inc.).
6
Cell Culture with Fetal Bovine Serum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fetal bovine serum (FBS) was obtained from Biowest (Nuaille, France). DMEM (high glucose) with L-glutamine, phenol red, and sodium pyruvate was obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Plastic dishes were obtained from TPP (Trasadingen, Switzerland). All other materials used were of the highest commercial grade.
7
Culturing COS7 and tsA201 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
COS7 and tsA201 cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) (High Glucose) (Wako) supplemented with 2 mM L-glutamine (Wako), 1 mM sodium pyruvate (Wako), 10% fetal bovine serum (FBS, Biosera) and 0.6% penicillin/streptomycin (Sigma) at 37°C in a humidified 5% CO2 incubator.
8
Culturing Mouse Embryonic Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In this study, we used mESC line E14tg2a (Riken Cell Bank, Japan).28 (link) mESCs were cultured at 37 °C and 5% CO2 on cell culture dishes coated with 0.1% gelatin from porcine skin (gel strength 300, type A; Sigma-Aldrich, USA). The cells were maintained with Glasgow Minimum Essential Medium (G-MEM; Wako, Japan) with 15% fetal bovine serum (FBS) (Sigma-Aldrich), 1 mmol/L sodium pyruvate (Wako), 1% MEM non-essential amino acids (Wako), 0.1 mmol/l 2-mercaptoethanol (Wako), and 1000 units/ml LIF (Wako). The cells were dissociated by TrypLE Express (Thermo Fisher Scientific, USA) and passaged at 7.0 × 103 cells/cm2 for 2-Day interval passage or 2.6 × 103 cells/cm2 for 3-Day interval passage.
9
Rcho-1 Cell Maintenance and Differentiation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rcho-1 cells were kindly provided by Professor Michael J. Soares (Department of Physiology, University of Kansas Medical Center, Kansas City, KS). Protocols for the maintenance and differentiation of Rcho-1 cells were in accordance with previous work16 (link). The cells were routinely cultured in standard growth medium (RPMI1640, Sigma-Aldrich, St. Louis, MO) containing 50 μM 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO), 1 mM sodium pyruvate (Wako, Osaka, Japan), and 20% fetal bovine serum. Rcho-1 cell differentiation was induced by culturing in NCTC-135 culture medium (Sigma-Aldrich, St. Louis, MO) containing 50 μM 2-mercaptoethanol, 1 mM sodium pyruvate, and 1% horse serum. For observation of Rcho-1 cell shape, we used CellMask (Thermo Fisher Scientific, Tokyo, Japan) according to the manufacturer’s protocol.
10
Metastatic Melanoma Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Two malignant melanoma cell lines, B16 and B16/BL6, were provided by the Riken Cell Bank (Ibaragi, Japan). B16/BL6 is a subline of B16 melanoma, with highly metastatic potential to the lungs of syngeneic C57BLi6N mice [16 (link), 17 (link)]. The culture medium consisted of Dulbecco’s modified Eagle’s medium (DMEM) (Wako, Osaka, Japan) with the addition of 10% fetal bovine serum (FBS) (Nichirei Biosciences, Tokyo), 100 IU/mL penicillin (Wako), 100 mg/mL streptomycin (Wako), and 0.5 mM sodium pyruvate (Wako). Cells were cultured at 37°C in 5% CO2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!