Dimension x pand plus clinical chemistry system

Seven Yorkshire pigs (Midwest Research Swine, Gibbon, MN) weighing 68.0 ± 4.9 kg were used in this study. To ensure health of the animal and the kidney, blood samples were taken prior to euthanasia for analysis of blood chemistry and complete blood count (CBC). All procedures were approved by the Regulatory Research Compliance Division of the US Army Institute of Surgical Research (USAISR). Specifically, a tissue sharing request to the Animal Care and Use Committee from USAISR was completed. This research was conducted in compliance with the Animal Welfare Act, the implementing Animal Welfare Regulations, and the principles of the Guide for the Care and Use of Laboratory Animals, National Research Council. The facility's Institutional Animal Care and Use Committee approved all research conducted in this study. The facility where this research was conducted is fully accredited by AAALAC International. For protein, creatinine, and BUN measurement, blood was collected into a BD Vacutainer tube with Lithium Heparin (Becton Dickinson and Company, Franklin Lakes, NJ) and centrifuged at 1,200 g for 10 min. Samples were analyzed on a Siemens Dimension® Xpand™ Plus clinical Chemistry system. For CBC analysis, specimens were collected in a BD Vacutainer tube containing K2 EDTA (Becton Dickinson and Company, Franklin Lakes, NJ) and analyzed with the Abbott Cell-Dyn® 3700 system.