Primary antibodies included polyclonal rabbit anti-Ac-p53 (Lys379) (Thermo Scientific), monoclonal rat anti-HDAC9 antibody (clone 45a7b5b), monoclonal mouse anti-FLAG M2 (Sigma), polyclonal rabbit anti-acetylated lysine (Millipore 06-933), polyclonal rabbit anti-BCL6 (ab19011), anti-CD45R(B220) (ab64100) and anti-CD3 (ab5690). Secondary antibodies included: biotinylated rabbit anti-rat secondary antibody (Vector Laboratories, PK-6104), biotinylated horse anti-mouse secondary (Vector Laboratories, PK6102) and biotinylated goat anti-rabbit secondary (Vector Laboratories, PK6101). Fluorochromes and chromogens included SA-HRP (Vector Laboratories, PK-6102), FITC fluorophore tyramide (Perkin Elmer, NEL741, NEL753), FITC-HRP (Perkin Elmer, NEF710), Cy3 fluorophore tyramide (Perkin Elmer, NEL741, NEL753), Cy5 fluorophore tyramide (Perkin Elmer, NEL745) and AEC (AbD Serotec, BUF019B).
Nel741
Manufactured by PerkinElmer
The NEL741 is a versatile piece of lab equipment from PerkinElmer. It is designed to perform a specific function within the laboratory setting, but a detailed description while maintaining an unbiased and factual approach is not available at this time.
Automatically generated - may contain errors
Lab products found in correlation
Mouse monoclonal anti flag m2, by Merck Group (1 mentions)
Nel753, by PerkinElmer (1 mentions)
Nef710, by PerkinElmer (1 mentions)
Pk 6102, by Vector Laboratories (1 mentions)
Goat anti rabbit hrp, by Agilent Technologies (1 mentions)
Goat anti mouse hrp, by Agilent Technologies (1 mentions)
Goat anti rat, by Thermo Fisher Scientific (1 mentions)
Sp7 osterix, by Abcam (1 mentions)
Nel744, by PerkinElmer (1 mentions)
2 protocols using nel741
1
Multicolor Immunohistochemistry Staining Protocol
2
Immunohistochemical Analysis of Limb Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Paraffin-embedded 7 μm sections of hind limb tissues were citrate-unmasked and stained with the following primary antibodies: polyclonal P-Smad 1/5 (1:100; (41D10) 9516, Cell Signaling Technology), polyclonal Sox9 (1:100, Santa Cruz Biotechnology, sc-20095), polyclonal Sp7/Osterix (1:100, Abcam ab22552), monoclonal GFP living Colors (1:100 Clontech 632381) or polyclonal GFP (1:200; Origene/Acris lab R1091P) and monoclonal CD140a (PDGFRα) (1:100 (APA5) ThermoFisher 17-1401-81). Secondary antibodies were as follows: goat-anti-rabbit HRP (P0448, DakoCytomation; 1:100), goat-anti-mouse HRP (P0447, DakoCytomation; 1:100) goat-anti-rat (ThermoFisher, 31470 1:100) HRP-coupled secondary antibodies and donkey-anti-goat-HRP (ThermoFisher, PA128664). The signal was tyramide signal amplified (TSA) with coupling to Cy3 (NEL744, Perkin Elmer; 1:100) or fluorescein ((NEL744 or NEL741 Perkin Elmer; 1:100)
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