5 bromo 2 deoxyuridine brdu incorporationassay kit

Proliferation of HepG2 cells after kaempferol treatment and/or miR-21 mimic
transfection were measured using 5-bromo-2′-deoxyuridine (BrdU) incorporation
assay kit (Sigma–Aldrich) in line with the manufacturer’s protocol. Briefly,
HepG2 cells were seeded, in triplicate, in 6-well plate (Thermo Fisher
Scientific) with a density of 1 × 10⁵ cells/well. BrdU solution was
added into each well of the plate before 50 μM kaempferol treatment by 4 h.
After kaempferol incubation for 24 h, BrdU positive(+) cells in each well was
counted under microscope (Nikon, Japan), which was proportional to cell
proliferation.

Cell proliferation was determined using a 5-bromo-2′-deoxyuridine (BrdU) incorporation assay kit (Sigma-Aldrich) following the manufacturer’s instructions. Briefly, 3 × 10⁴ SH-SY5Y cells per well were seeded into 24-well plates. BrdU solution was added into each well before 20 nM TPL treatment. After culturing for 24 h, the number of BrdU⁺ cells of each group was counted, which was proportional to cell proliferation.