Dcfda kit

Manufactured by Merck Group

Sourced in China

The DCFDA (2',7'-Dichlorofluorescin diacetate) Kit is a laboratory product designed to measure intracellular oxidative stress. The kit contains the DCFDA chemical compound, which is a fluorogenic dye that can detect reactive oxygen species within cells. This product allows researchers to quantify the levels of oxidative stress in a variety of cell types.

Automatically generated - may contain errors

Lab products found in correlation

Lipid peroxidation mda assay kit, by Merck Group (1 mentions) Sod assay kit, by Merck Group (1 mentions)

Close spelling variants of this product

2-7-Dichlorodihydrofluorescein diacetate (DCFDA) fluorescence kit (2 citations)

3 protocols using dcfda kit

Evaluating Oxidative Stress in HMEC-1

Check if the same lab product or an alternative is used in the 5 most similar protocols

To determine whether PM2.5 affected the ROS levels of HMEC-1, a 2′,7′-dichlorofluorescin diacetate (DCFDA) kit (Sigma-Aldrich; Merck KGaA) was used. Curcumin/PM2.5 treated HMEC-1 (2×10⁵ cells/well) were added to DCFDA (20 µM) for 20 min, washed with PBS and then the ROS expression was analyzed by flow cytometry All experiments were repeated three times.
The malondialdehyde (MDA) level and superoxide dismutase (SOD) activity of HMEC-1 were detected by a Lipid Peroxidation (MDA) assay kit (Sigma-Aldrich; Merck KGaA) and SOD assay kit (Sigma-Aldrich; Merck KGaA), respectively.

Shi J., Deng H, & Zhang M. (2017). Curcumin pretreatment protects against PM2.5-induced oxidized low-density lipoprotein-mediated oxidative stress and inflammation in human microvascular endothelial cells. Molecular Medicine Reports, 16(3), 2588-2594.

+ Open protocol

+ Expand

Intracellular ROS Quantification via DCFDA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Intracellular ROS was assessed using the DCFDA kit (Sigma) as previously described [8 (link)]. Kidney cortex sections were labeled for ROS according to manufacturer's protocol. Samples were imaged using fluorescence microscope. Image J was used to quantify pixel density in glomerular. ROS generated by mitochondria were also measured with DCFDA kit.

Shui G.X., Sang D., Yin X., Cai Y, & Sun W. (2017). Dahuang Fuzi Decoction Attenuates Renal Fibrosis and Ameliorates Mitochondrial Dysfunction in Chronic Aristolochic Acid Nephropathy. Evidence-based Complementary and Alternative Medicine : eCAM, 2017, 9536458.

+ Open protocol

+ Expand

Evaluating Hippocampal ROS Levels

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The levels of ROS in hippocampus were evaluated by using 2, 7-dichlorofluorescindiacetate (DCFDA) Kit (Sigma, China). Briefly, hippocampus homogenate was washed with PBS, mixed with DCFDA (dissolved with methanol) and then incubated in water for 15 min at 37 °C. After that, the samples were stained by DAPI. ROS images were measured using a DCFH-DA fluorescence probe under a fluorescence microscope with a magnification of 400×. Fluorescence intensity was calculated using a fluorometer at 488 nm excitation wavelength and 525 nm emission wavelength (Hemmati et al. 2018 (link)).

Wang W., Zheng L., Xu L., Tu J, & Gu X. (2020). Pinocembrin mitigates depressive-like behaviors induced by chronic unpredictable mild stress through ameliorating neuroinflammation and apoptosis. Molecular Medicine, 26, 53.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!