Rosettesep human progenitor cell basic pre enrichment antibody cocktail

Manufactured by STEMCELL

RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment is an antibody cocktail designed for the isolation of human progenitor cells from a variety of starting materials. The cocktail depletes unwanted cells, allowing the target progenitor cells to be recovered.

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Lab products found in correlation

Stemspan sfem 2 medium, by STEMCELL (2 mentions) Stemspan erythroid expansion supplement, by STEMCELL (1 mentions)

Spelling variants of this product

RosetteSep (157 citations) RosetteSep antibody cocktail (5 citations)

2 protocols using rosettesep human progenitor cell basic pre enrichment antibody cocktail

Expansion of Saudi Progenitor Cells

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According to the manufacturer's instructions, peripheral blood from Saudi patients was drawn into a blood collection tube containing EDTA and processed with the RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment antibody cocktail (Stem Cell Technologies Catalog #15226). 1 million cells were cultivated for 8 days after PBMC separation and isolation in StemSpan™ SFEM II medium (Stem Cell Technologies Catalog #09605) with 1X StemSpan™ Erythroid Expansion Supplement (Stem Cell Technologies Catalog #02692).

Alowaysi M., Al-Shehri M., Badkok A., Attas H., Aboalola D., Baadhaim M., Alzahrani H., Daghestani M., Zia A., Al-Ghamdi K., Al-Ghamdi A., Zakri S., Aouabdi S., Tegner J, & Alsayegh K. (2023). Generation of iPSC lines (KAIMRCi003A, KAIMRCi003B) from a Saudi patient with Dravet syndrome carrying homozygous mutation in the CPLX1 gene and heterozygous mutation in SCN9A. Human Cell, 37(2), 502-510.

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Isolation and Expansion of Erythroid Progenitors

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Peripheral blood was collected from the donor into EDTA-containing blood collection tube and treated with RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment antibody cocktail according to the manufacturer’s instructions (StemCell Technologies Catalog#15226). After PBMCs separation and isolation, 1 million cells were cultured for 8 days in StemSpan™ SFEM II medium (StemCell Technologies Catalog #09605) supplemented with 1X StemSpan™ Erythroid Expansion Supplement (StemCell Technologies Catalog #02692).

Alowaysi M., Lehmann R., Al-Shehri M., Baadhaim M., Alzahrani H., Aboalola D., Zia A., Malibari D., Daghestani M., Alghamdi K., Haneef A., Jawdat D., Hakami F., Gomez-Cabrero D., Tegner J, & Alsayegh K. (2023). HLA-based banking of induced pluripotent stem cells in Saudi Arabia. Stem Cell Research & Therapy, 14, 374.

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