7d camera

Developmental stages were observed from the crossings at 0, 2, 8, 17, 24, and 37 h following the developmental cycle51 (link)52 . Photos were taken at each stage using an Olympus microscope with an Olympus DP72 CCD camera (Olympus Corporation, Tokyo, Japan) and a Canon 7D camera (Figs. 6, 7, 8), and survival rates were observed under an Olympus CX31 microscope (Olympus Corporation, Tokyo, Japan). Fertilization success, the percentage of cells clearly reaching every step of the normal cycle in non-aberrant shape (prawn chip, donuts, and pear, per51 (link)) (Fig. 4), and the rates of aberrant development (Fig. 6, 7, 8) were recorded. Cycle time was established by performing fertilizations at ambient temperature (Fig. 4). The experiment was conducted until the appearance of the first motile stage (pear-shaped larvae).

The inclusion criteria were doxycycline exposure at the age of 0–7.9 years and a minimum age of 8 years at the time of dental examination. These age limits were selected based on the known dental mineralization timeline of tooth development.⁹ (link) We reviewed the medical records of 1132 children hospitalized due to suspicion of CNS infection during 1994–2015 at the Department of Paediatrics and Adolescent Medicine, Turku University Hospital. The inclusion criteria were met by 56 children, of whom 39 agreed to participate in the study.
Details of treatment were collected from medical records. Dental examinations were performed by an experienced paediatric dentist (M. N.). Dental developmental stage was noted. The focus of the clinical examination was to detect tetracycline-like staining in the forms of diffuse discoloured bands of tooth crowns and enamel hypoplasia. Teeth were photographed using a mouth mirror from five perspectives with a Canon 7D camera (Canon, Tokyo, Japan). Photographs were evaluated by a second experienced paediatric dentist (S. A.), blind to the results of the first examiner.