Secreted cytokines were assessed by two different methods. At 48 and 96 h post-activation, cell media from T-lymphocyte cultures was analyzed using the mouse Th1/Th2/Th17 cytometric bead array (BD Biosciences #560485, San Jose, CA) as per manufacturer's instructions. Analysis beads were analyzed on a LSRII flow cytometer for quantification of specific cytokines. At 96 h post-activation, cell media was also analyzed using a Meso Scale Discovery 35 U-Plex Mouse Biomarker Group (#K15083K-1, Rockville, MD). Samples were analyzed using a Meso Scale QuickPlex SQ 120, and normalized to cell number.
Meso scale discovery 35 u plex mouse biomarker group
Manufactured by Mesoscale
Sourced in United States
The Meso Scale Discovery 35 U-Plex Mouse Biomarker Group is an analytical instrument designed to measure multiple biomarkers in mouse samples. It utilizes electrochemiluminescence technology to quantify a panel of 35 different mouse-specific analytes simultaneously from a single sample. The core function of this product is to provide a comprehensive and efficient platform for multiplex biomarker analysis in mouse-based research and studies.
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2 protocols using meso scale discovery 35 u plex mouse biomarker group
1
Cytokine Secretion Assessment in T-Lymphocytes
2
Cytokine Profiling via MSD U-Plex
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Analysis of circulating levels of cytokines was performed using a Meso Scale Discovery 35 U-Plex Mouse Biomarker Group (#K15083K-1, Rockville, MD, USA) per manufacturer’s instructions. Samples were assessed using a Meso Scale QuickPlex SQ 120, and analyzed using Meso Scale Discovery software.
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