Malondialdehyde mda content assay kit

Gelatin type B, tragacanth gum, Curcumin, Glacial acetic acid, Sodium sulfate, 37% formaldehyde, Chondroitin sulfate, EDC, NHS were purchased from Soleberg Reagent. Collagen (Type II, Chicken, Protein 60%, Yuanye), Fuchsin Complete Adjuvant (FCA), Fuchsin Incomplete Adjuvant (FICA) was purchased from Genye Reagent Company. Nitrendipine was obtained from Aladdin Chemical Reagent Co. CCK-8 kit, ROS kit, malondialdehyde (MDA) content assay kit, total glutathione (GSH-Px) content assay kit, superoxide dismutase (SOD) content assay kit and H&E kit were purchased from Beyotime Anti-HIF-1α, Anti-iNOS, anti-Arg-1, anti-IL-1β, anti-IL-10 were purchased from Abcam. RAW264.7 cells were obtained from Chinese Academy of Sciences.

Hyperoside was purchased from MedChemExpress (Monmouth Junction, NJ, USA) as a crystalline powder with a purity of 99.56%. It was dissolved in DMSO and prepared into a mother liquor of 100 mM. DOX was purchased from MedChemExpress as a crystalline powder with a purity of 99.48%. It was dissolved in DMSO and prepared as a 10 mM mother liquor. They were diluted to the desired concentration when used. One-step terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling (TUNEL) apoptosis assay kit, reactive oxygen species (ROS) assay kit and malondialdehyde (MDA) content assay kit purchased from Beyotime Biotech Inc (Shanghai, China). Catalase (CAT) activity assay kit, superoxide dismutase (SOD) activity assay kit and reduced glutathione (GSH) content assay kit purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). Cell counting kit-8 (CCK-8) was purchased from Apexbio (Houston, TX, USA). Fetal bovine serum (Bovine serum) was purchased from Sigma (Santa Clara, CA, USA). HL-1 cells and MDA-MB-231 cells were obtained from Shanghai Fuheng Biotechnology Co., Ltd. (Shanghai, China).

Tetraethyl orthosilicate (TEOS), potassium permanganate (KMnO₄), sodium carbonate (Na₂CO₃), ethanol, methanol, acetone, and paraformaldehyde were purchased from Sinopharm Holding Chemical Reagent Company, Ltd. (Shanghai, China). Dimethyl sulfoxide (DMSO), phloretin, carboxymethylcellulose sodium, and 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT) were obtained from Aladdin Chemical Reagent Co. ROS assay kit, malondialdehyde (MDA) content assay kit, superoxide dismutase (SOD) content assay kit, total glutathione (GSH-Px) content assay kit, and Hematoxylin-Eosin/HE staining kit were bought from Beyotime Biotechnology Co. Anti-HIF-1α, Anti-iNOS, anti-cox-2, anti-IL-1β, anti-IL-10, anti-bax, anti-bcl-2, anti-Cl caspase-3, anti-β-Tubulin, and anti-actin were purchased from Abcam. PC12 cells and BV2 cells were obtained from the cell bank of Chinese Academy of Sciences.