Primary antibodies against Snail1 (cat. no. 125918) were purchased from GeneTex, Inc. (Irvine, CA, USA). Primary antibodies against Wnt1 (ab85060), Wnt8a (ab130930), phosphorylated glycogen synthase kinase 3β (P-GSK3β; ab130937) and GAPDH (ab37168) were obtained from Abcam (Cambridge, UK). Primary antibodies against Wnt3a (bs1700R) and Wnt5a (bs1948R) were purchased from BIOSS (Beijing, China). The primary antibody against Wnt11 (sc50360) was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Antibodies against β-catenin (BA0426) and β-actin (BM0627) were obtained from Wuhan Boster Biological Technology, Ltd. (Wuhan, China). The antibody against GSK3β (22104-1-AP) was purchased from Wuhan Sanying Biotechnology (Wuhan, China). The bicinchoninic acid (BCA) protein assay kit was purchased from Beyotime Institute of Biotechnology (Haimen, China). TRIzol (252250AX) was obtained from Aidlab Biotechnologies Co., Ltd. (Beijing, China). M-MLV Reverse Transcriptase (RNase H; CO2010A) and ddH2O (DNase/RNase Free; C1D230A) were purchased from GeneCopoeia, Inc. (Rockville, MD, USA) and RNase Inhibitor (I21222) was obtained from TransGen Biotech, Inc. (Beijing, China).
Bs1948r
Manufactured by Bioss Antibodies
Sourced in China
The Bs1948R is a multi-channel peristaltic pump designed for diverse laboratory applications. It features precise flow rate control, adjustable pump heads, and durable materials for reliable performance. The core function of the Bs1948R is to accurately and consistently transfer liquids in a variety of laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using bs1948r
1
Western Blot Analysis of Wnt Pathway Proteins
2
Comprehensive Western Blotting Methodology
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Western blotting was performed following procedures described previously [25 (link)]. The following primary antibodies were used: anti-WNT5A (1:1000, bs-1948R, BIOSS), anti-FLAG (1:1000, 66008-3-Ig, Proteintech), anti-HDAC7 (1:1000, #33418, CST), anti-SNAIL (1:1000, 13099-1-AP, Proteintech), anti-E-cadherin (1:1000, #14472, CST), anti-SLUG (1:1000, #9585, CST), anti-vimentin (1:1000, 10366-1-AP, Proteintech), anti-TWIST1 (1:1000, 25465-1-AP, Proteintech), anti-HDAC1 (1:1000, #5356, CST), anti-HDAC2 (1:1000, #5113, CST), anti-HDAC3 (1:1000, #3949, CST), anti-HDAC4 (1:1000, 17449-1-AP, Proteintech), anti-HDAC5 (1:1000, 16166-1-AP, Proteintech), anti-HDAC6 (1:1000, 12834-1-AP, Proteintech), anti-HDAC8 (1:1000, E-AB-14127, Elabscience), anti-HDAC9 (1:1000, 67364-1-Ig, Proteintech), anti-HDAC10 (1:1000, 24913-1-AP, Proteintech), anti-HDAC11 (0.3 µg/mL, PA5-79351, Thermo), and anti-β-actin (1:1000, #3700, CST). The secondary antibodies used were HRP-linked anti-IgG antibodies (1:5000, Zhongshan Company, Beijing, China).
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