After 48 h of transfection, cells were collected and washed with cold PBS. Cells were resuspended in 300 μL of a propidium iodide (PI) mixture (containing 200 μg/ml PI, 100 μg/ml RNase and 0.2%Triton X-100) and incubated at 4°C for 30 min. Fluorescent signals were measured at the FL-2 channel using CFlow Plus package from Accuri C6. The data obtained analyzed using ModFit software. Experiments were performed in triplicates.
Cflow plus package
Manufactured by BD
The CFlow Plus package is a laboratory equipment product from BD. It serves as a core function in laboratory settings, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cflow plus package
1
Cell Cycle Analysis by Flow Cytometry
2
Cell Cycle Analysis by Flow Cytometry
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Cells were collected and washed with pre-cold PBS. For 2 × 105 cells resuspended with 400 μl propidium iodide (PI) mixture (including 200 μg/ml PI, 100 μg/ml RNase, 0.2%Triton X-100) and incubated at 4 °C for 30 min. Fluorescent signals were measured at FL-2 channel with CFlow Plus package from Accuri C6 and analyzed with ModFit software.
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