Trypan blue stain solution

PC12 cells were purchased from the American Type Culture Collection (USA and Canada). Dulbecco's modified Eagle medium (DMEM), ribonuclease A and o-phenylenediamine dihydro chloride (OPD) were obtained from Sigma-Aldrich (St. Louis, MO, USA). NP 2 EO was purchased from Tokyo Chemical Industry Corporation (Tokyo, Japan). Fetal bovine serum (FBS) was obtained from HyClone (Rockville, MD USA). Monoclonal antibody against cytochrome c, and polyclonal antibody against Bcl-2 were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Nonradioactive cytotoxicity assay kit and purified horseradish peroxidase conjugate of goat anti-mouse IgG (H+L) were obtained from Promega (Madison, WI, USA). Polyclonal antibody against Bax was provided by Calbiochem (San Diego, CA, USA). Anti-rabbit immunoglobulin peroxidase conjugate was obtained from Amersham Bioscience UK Limited (UK). Amersham TM ECL TM western blotting detection reagents were obtained from GE Healthcare UK Limited (UK). The High Pure PCR template Preparation Kit for genomic DNA and Proteinase K was obtained from Roche Diagnostics (Basel, Switzerland). Trypan blue stain solution (0.5%) was obtained from Bio-Rad (Hercules, CA, USA). The Cytochrome c Releasing Apoptosis Assay kit was from Medical and Biological Laboratories Co., Ltd (Japan). All other chemicals were analytical grade.