Chromatin was extracted from naive CD4+Foxp3− T cells (1 × 106 cells) polarized for 24–72 hours under various conditions after fixation with formaldehyde. Anti-Histone H3ac (39139, 7µl), anti-Histone H4ac (39243, 6µl), anti-Histone H3 (trimethyl K9) (61013, 7µl), anti-Histone H3 (dimethyl K27) antibody (39245, 5µl; all from Active Motif), anti-HDAC1 (ab7028, 5µg), anti-Smad3 (ab28379, 5µg), anti-Histone H3 (trimethyl K27) (ab6002, 5µl), anti-Histone H3 (acetyl K27) (ab4729, 3µl), anti-Histone H3 (acetyl K9) (ab4441, 4µl; all from Abcam), anti-dimethyl-Histone H3 (diMe-Lys9) (D5567, 5µl; Sigma- Aldrich), anti-p50 (sc-1192X, 5µg), anti-p300 (sc-585X, 5µg), anti-STAT6 (SC-981X, 5µg), control mouse IgG (sc-2025, 5µg; all from Santa Cruz Technology) and purified goat IgG (02–6202, 5µg; Life Technologies) were used for the immunoprecipitation of chromatin with an EZ ChIP kit (17–371; EMD Millipore) according to the manufacturer's instructions. The precipitated DNA was measured by real-time PCR using SsoAdvanced Universal SYBR Green Supermix (172–5275; Bio-Red). Real-time PCR primer sequences are listed in Supplementary Table 2 . Data are presented as relative binding based on normalization to input DNA50 (link).
Anti p300 sc 585x
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-p300 (sc-585X) is a primary antibody produced by Santa Cruz Biotechnology. It is designed to detect p300 protein expression in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti h3k27ac ab4729, by Abcam (2 mentions)
Anti stat6 sc 981x, by Santa Cruz Biotechnology (1 mentions)
Anti histone h3ac, by Active Motif (1 mentions)
Anti histone h3 dimethyl k27 antibody, by Active Motif (1 mentions)
Anti histone h4ac, by Active Motif (1 mentions)
Purified goat igg, by Thermo Fisher Scientific (1 mentions)
Ez chip kit, by Merck Group (1 mentions)
Ab4441, by Abcam (1 mentions)
Control mouse igg, by Santa Cruz Biotechnology (1 mentions)
Ab7028, by Active Motif (1 mentions)
Anti med1 a300 793a, by Fortis Life Sciences (1 mentions)
Anti h3k27me3, by Merck Group (1 mentions)
Anti h3k4me1, by Abcam (1 mentions)
Anti c ebpα sc 61x, by Santa Cruz Biotechnology (1 mentions)
Anti pparγ sc 7196x, by Santa Cruz Biotechnology (1 mentions)
Anti c ebpβ sc150x, by Santa Cruz Biotechnology (1 mentions)
Anti h3k4me2, by Abcam (1 mentions)
Anti pol 2, by Merck Group (1 mentions)
Anti h3k4me3, by Merck Group (1 mentions)
Anti ctcf, by Merck Group (1 mentions)
3 protocols using anti p300 sc 585x
1
Chromatin Immunoprecipitation of Activated T Cells
2
Investigating Brd4 Regulation Using Lentiviral shRNA
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The lentiviral shRNA plasmids pLKO.1 targeting mouse Brd4 (clone IDs TRCN0000311976, TRCN0000088480, and TRCN0000088481) were purchased from Sigma. Anti-RbBP5 (A300–109A), anti-Brd4 (A301–985A100), and anti-MED1 (A300–793A) were from Bethyl Laboratories. Anti-C/EBPα (sc-61X), anti-C/EBPβ (sc-150X), anti-PPARγ (sc-7196X), and anti-p300 (sc-585X) were from Santa Cruz Biotechnology. Anti-H3K4me1 (ab8895), anti-H3K4me2 (ab7766), and anti-H3K27ac (ab4729) were from Abcam. Anti-Pol II (17-672) was from Millipore. For western blot analysis, all antibodies were diluted to 1 μg ml−1. Uncropped blots are available in the Supplementary Figure 7 .
3
Antibody Characterization for Epigenetic Research
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The following antibodies were used in this study. Anti-C/EBPα (sc-61X, lot: G1112), anti-C/EBPβ (sc-150X, lot: L2910), anti-PPARγ (sc-7196X, lot: H1512), anti-p300 (sc-585X, lot: E2209) were from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-CBP (7389S, lot: 1) was from Cell Signaling Technology (Danvers, MA, USA). Anti-MED1/TRAP220 (A300-793A, lot: 2), anti-RbBP5 (AA300-109A), anti-Menin (A300-105A) were from Bethyl Laboratories (Montgomery, TX, USA). Anti-EBF2 (AF7006, lot: CEUA0113081) was from R&D Systems (Minneapolis, MN, USA). Anti-CTCF (07-729, lot: 2054523), Anti-Pol II (17-672, lot: 2015592), anti-H3K4me3 (07-473, lot: 1974075) and Anti-H3K27me3 (07-449, lot: DAM1387952) were from Millipore (Billerica, MA, USA). Anti-H3K4me1 (ab8895, lot: GR61280-1), anti-H3K4me2 (ab7766, lot: GR102810-1), anti-H3K9me2 (ab1220, lot: 957002), anti-H3K27ac (ab4729, lot: GR81163-1), anti-H3K36me3 (ab9050, lot: 947436) were from Abcam (Cambridge, MA, USA). Anti-UTX was described previously (21 (link)). Anti-MLL4 and anti-SET1A were described previously (22 ).
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