7890b gc 7697a headspace 5977a

Manufactured by Agilent Technologies

The 7890B GC, 7697A Headspace, and 5977A Mass Spectrometer are analytical instruments designed for gas chromatography and mass spectrometry analysis. The 7890B GC is a gas chromatograph used for the separation and analysis of volatile compounds. The 7697A Headspace is a sample introduction system that prepares volatile samples for analysis by the gas chromatograph. The 5977A Mass Spectrometer is a high-performance mass spectrometer used for the identification and quantification of chemical compounds.

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Lab products found in correlation

Masshunter quantitative analysis vers b 08.00 build 8 0 593 0, by Agilent Technologies (2 mentions) Db 624 ui, by Agilent Technologies (2 mentions) Cannabidiol, by Restek (1 mentions) Cannabidivarin, by Restek (1 mentions) Cannabichromene, by Restek (1 mentions) Cannabidiolic acid, by Restek (1 mentions) Cannabigerol, by Restek (1 mentions) Cannabigerolic acid, by Restek (1 mentions) Cannabinol, by Restek (1 mentions) 1260 uplc system, by Agilent Technologies (1 mentions)

2 protocols using 7890b gc 7697a headspace 5977a

Comprehensive Terpene and Cannabinoid Profiling

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(1) Terpene profiling. Terpenoid analyses (DigiPath Laboratories) were carried out on an Agilent 7890B GC/7697A Headspace/5977A mass spectrophotometer with a DB-624UI and Agilent 5181–8818 split/splitless liner. Injector port temperature was 250°C with a transfer line, valve oven and needle temperature of 180°C. Carrier gas was helium at a flow of 33.0 cm/s. The MS detector was set to scan with a range from 50 to 300 m/z. The instrument was controlled by Agilent Masshunter quantitative analysis (Vers. B.08.00 Build 8.0.593.0). Certified reference standards were from Restek (Bellefonte, PA) with Masshunter library confirmation.
(2) Strain-inspired mixture: Cannabidivarin (CBDV), Cannabichromene (CBC), Cannabidiol (CBD), Cannabidiolic Acid (CBDA), Cannabigerol (CBG), Cannabigerolic Acid (CBGA), Cannabinol (CBN), alpha-Bisabolol, alpha-Humulene, alpha-Pinene, beta-Caryophyllene, beta-Myrcene, (+)-beta-Pinene, Camphene, Limonene, Linalool, Nerolidol. (3) Cannabinoid mixture: Cannabidivarin (CBDV), Cannabichromene (CBC), Cannabidiol (CBD), Cannabidiolic Acid (CBDA), Cannabigerol (CBG), Cannabigerolic Acid (CBGA), Cannabinol (CBN). (4) Terpene mixture: alpha-Bisabolol, alpha-Humulene, alpha-Pinene, beta-Caryophyllene, beta-Myrcene, (+)-beta-Pinene, Camphene, Limonene, Linalool, Nerolidol.

Jansen C., Shimoda L.M., Kawakami J.K., Ang L., Bacani A.J., Baker J.D., Badowski C., Speck M., Stokes A.J., Small-Howard A.L, & Turner H. (2019). Myrcene and terpene regulation of TRPV1. Channels, 13(1), 344-366.

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Analytical Techniques for Cannabinoid and Terpenoid Profiling

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Cannabinoid assays were run on an Agilent Technologies 1260 UPLC system (Santa Clara, CA) equipped with a G4212A DAD, G1316C temperature-controlled column compartment, G4226A autosampler, and G4204A quaternary pump. Cannabinoids were separated on a Poroshell 120 EC-C18 column with a Poroshell 120 EC-C18 guard column. The UPLC runs OpenLab CDS ChemStation Rev C. 01.06 (61) software (Agilent Technologies). Terpenoid analyses were carried out on an Agilent 7890B GC/7697A Headspace/5977A mass spectrophotometer with a DB-624UI and Agilent 5181–8818 split/splitless liner. Injector port temperature was 250°C with a transfer line, valve oven, and needle temperature of 180°C. Carrier gas was helium at a flow of 33.0 cm/sec. The mass spectrometer detector was set to scan with a range from 50 to 300 m/z. The instrument was controlled by Agilent MassHunter Quantitative Analysis (Vers. B.08.00 Build 8.0.593.0). Certified reference standards were from Restek (Bellefonte, PA) with MassHunter library confirmation.

Reimann-Philipp U., Speck M., Orser C., Johnson S., Hilyard A., Turner H., Stokes A.J, & Small-Howard A.L. (2020). Cannabis Chemovar Nomenclature Misrepresents Chemical and Genetic Diversity; Survey of Variations in Chemical Profiles and Genetic Markers in Nevada Medical Cannabis Samples. Cannabis and Cannabinoid Research, 5(3), 215-230.

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