The chemicals and regents used were: Choline chloride, sodium phosphate dibasic dodecahydrate, diethyl ether, iron chloride hexahydrate, ferrous sulfate, sulfuric acid (98%), hydrochloric acid (36%), ammonium hydroxide solution, and tetraethyl orthosilicate (Sinopharm Chemical Reagent Co. Ltd., Shanghai, China); sodium metavanadate, sodium molybdate dihydrate, dimethyl sulfoxide, anhydride, pyridine, and oleic acid (Shanghai Macklin Biochemical Technology Co., Ltd., Shanghai, China); Tetrahydrofuran, ethanol, and toluene (Tianjin Hengxing Chemical Reagent Co., Ltd., Tianjin, China); lactic acid, and potassium bromide (Tianjin Kemiou Chemical Reagents Co. Ltd., Tianjin, China).
Sodium phosphate dibasic dodecahydrate
Manufactured by Sinopharm
Sourced in China
Sodium phosphate dibasic dodecahydrate is a chemical compound with the formula Na2HPO4·12H2O. It is a crystalline solid that is used as a buffering agent and a source of phosphate ions in various laboratory and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Sinopharm (4 mentions)
Sodium chloride (nacl), by Sinopharm (4 mentions)
Sodium hydroxide (naoh), by Sinopharm (2 mentions)
Potassium chloride (kcl), by Sinopharm (2 mentions)
Sodium phosphate monobasic dihydrate, by Sinopharm (2 mentions)
Ammonium hydroxide solution, by Sinopharm (1 mentions)
Choline chloride, by Sinopharm (1 mentions)
Oleic acid, by Maclin Biochemical Technology (1 mentions)
Dimethyl sulfoxide (dmso), by Maclin Biochemical Technology (1 mentions)
Sodium molybdate dihydrate, by Maclin Biochemical Technology (1 mentions)
Pyridine, by Maclin Biochemical Technology (1 mentions)
Sulfuric acid, by Sinopharm (1 mentions)
Ferrous sulfate, by Sinopharm (1 mentions)
Tetraethyl orthosilicate, by Sinopharm (1 mentions)
Diethyl ether, by Sinopharm (1 mentions)
Iron chloride hexahydrate, by Sinopharm (1 mentions)
Potassium phosphate monobasic, by Sinopharm (1 mentions)
Sodium acetate, by Thermo Fisher Scientific (1 mentions)
O phenylenediamine, by Sinopharm (1 mentions)
Acetic acid, by Sinopharm (1 mentions)
7 protocols using sodium phosphate dibasic dodecahydrate
1
Synthesis of Inorganic Nanostructures
2
Colorimetric Enzyme-linked Immunoassay Reagents
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CuSO4·5H2O (99.0%), o-phenylenediamine (OPD, 98.5%), H2O2 (30.0%), ammonium chloride (NH4Cl, 99.5%), sodium phosphate
dibasic dodecahydrate (Na2HPO4·12H2O, 99.0%), potassium phosphate monobasic (KH2PO4, 99.5%), sodium chloride (NaCl, 99.5%), potassium chloride
(KCl, 99.5%), acetic acid (HAc, 99.5%), phosphoric acid (H3PO4, 85%), boric acid (H3BO3, 99.5%),
sodium hydroxide (NaOH, 96%), and hydrochloric acid (HCl, 36.0–38.0%)
were purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai.
Sodium acetate (NaAc, 99%) was bought from Acros Organics, Belgium.
DA·HCl (98%) and 3,3′,5,5′-tetramethylbenzidine
(TMB) (98%) were purchased from Aladdin Industrial Inc., Shanghai.
2-(N-Morpholino)ethanesulfonic acid (MES, 99%) was
purchased from Macklin Biochemical Co. Ltd. Shanghai. All these chemicals
were used directly without any further purification. All aqueous solutions
were prepared in ultrapure water with a resistivity of 18.2 MΩ
cm–1 (Millipore Simplicity). The composition and
the pH range of MES, Britton–Robison (BR), and phosphate-buffered
saline (PBS) buffer solutions are listed inTable S1 in the SI.
dibasic dodecahydrate (Na2HPO4·12H2O, 99.0%), potassium phosphate monobasic (KH2PO4, 99.5%), sodium chloride (NaCl, 99.5%), potassium chloride
(KCl, 99.5%), acetic acid (HAc, 99.5%), phosphoric acid (H3PO4, 85%), boric acid (H3BO3, 99.5%),
sodium hydroxide (NaOH, 96%), and hydrochloric acid (HCl, 36.0–38.0%)
were purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai.
Sodium acetate (NaAc, 99%) was bought from Acros Organics, Belgium.
DA·HCl (98%) and 3,3′,5,5′-tetramethylbenzidine
(TMB) (98%) were purchased from Aladdin Industrial Inc., Shanghai.
2-(N-Morpholino)ethanesulfonic acid (MES, 99%) was
purchased from Macklin Biochemical Co. Ltd. Shanghai. All these chemicals
were used directly without any further purification. All aqueous solutions
were prepared in ultrapure water with a resistivity of 18.2 MΩ
cm–1 (Millipore Simplicity). The composition and
the pH range of MES, Britton–Robison (BR), and phosphate-buffered
saline (PBS) buffer solutions are listed in
3
Preparation of Biochemical Reagents
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Sodium chloride (NaCl, 99.5%), sodium phosphate dibasic dodecahydrate (Na2HPO4·12H2O, 99%), potassium chloride (KCl, 99.5%), potassium phosphate dibasic anhydrous (KH2PO4, 99%) and zinc acetate dihydrate were purchased from Sinopharm Chemical Reagent Co., Ltd., Xi’an, China. Trichloroacetic acid (TCA), tyrosine, casein, N,N-Dimethylformamide (DMF) and tetrahydrofuran (THF), 1,4-dioxane were purchased from Aladdin Reagent Co., Ltd., Shanghai, China. Trypsin was purchased from Tci Reagent Co., Ltd., Shanghai, China. Deionized (DI) water was used for all aqueous solutions and tests.
4
Synthesis and Antioxidant Evaluation of Triisobutyryl Gallic Acids
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Triisobutyryl gallic acids were synthesized according to previous work [16 (link)]. Sodium bicarbonate, sodium phosphate dibasic dodecahydrate, sodium chloride, sodium phosphate monobasic dihydrate, anhydrous magnesium sulfate, alcohol, ethyl acetate, petroleum ether, toluene, hydrochloric acid, anhydrous ethanol, and methanol were obtained from Sinopharm Chemical Reagent (Shanghai, China). Phytol, 1-eicosanol, N,N-dicyclohexylarbodiimide (DCC), gallic acid, butylated hydroxy anisole (BHA), 2,6-di-tert-butyl-4-methylphenol (BHT), tert-butylhydroquinone (TBHQ), 2,2-diphenyl-1-picrylhydrazyl (DPPH), triethylamine, 4-dimethyaminopyridine (DMAP), hydrazinium hydrate solution (80%), glycerol trioleate, isobutyric anhydride, Tween-80, lecithin, 2,2′-azobis(2-methylpropionitrile) (AIBN), 2,2′-azobis-(2-methylpropionamidine), and dihydrochloride (AAPH) were purchased from Aladdin Reagent (Shanghai, China). Phytanol was obtained from Tokyo Chemical Industry. A thiobarbituric acid reactive substance kit was purchased from Suzhou Grace Biotechnology Co., LTD. All chemicals were of analytical grade.
5
Determination of Peroxidase Activity in Fishery Products
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Hydrogen peroxide (H2O2, 30%), horseradish peroxidase (HRP, freeze-dried powder, >200 units/mg), N,N-diethyl-1,4-phenylenediammonium sulfate (DPD), L-tyrosine and L-phenylalanine were purchased from Aladdin (Shanghai, China). FeCl3 and Nafion (10% in water) were purchased from Sigma-Aldrich (Shanghai, China). Tetrakis(4-carboxyphenyl)porphyrin (H2TCPP) was purchased from TCI (Shanghai, China). Oxalic acid dehydrate was purchased from Mackin (Shanghai, China). ZrClO2·8H2O, benzoic acid, DMF, glucose, ascorbic acid, potassium dihydrogen phosphate, lithium carbonate, sodium phosphate monobasic dihydrate, sodium phosphate dibasic dodecahydrate, magnesium sulfate heptahydrate, and calcium chloride dehydrate were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All the experimental water comes from the Milli-Q reference system (Millipore, America) unless stated otherwise.
Fishery product samples were Todarodes pacificus belonging to the family Ommastrephidae; Larimichthys polyactis belonging to the family Sciaenidae; Pennahia argentata belonging to the family Sciaenidae. The above three kinds of fresh fishery products were purchased in a local market (Xincheng Market, No. 95 Jindao Road, Dinghai District, Zhoushan 316021, China) and brought into the laboratory no later than 1 h in ice.
Fishery product samples were Todarodes pacificus belonging to the family Ommastrephidae; Larimichthys polyactis belonging to the family Sciaenidae; Pennahia argentata belonging to the family Sciaenidae. The above three kinds of fresh fishery products were purchased in a local market (Xincheng Market, No. 95 Jindao Road, Dinghai District, Zhoushan 316021, China) and brought into the laboratory no later than 1 h in ice.
6
Synthesis and Characterization of Ru(bpy)3Cl2-Doped Silica Nanoparticles
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Tris (2,2′-bipyridyl) dichlororuthenium(ii ) hexahydrate (Ru(bpy)3Cl2·6H2O, 99.95%), tetraethyl orthosilicate (TEOS, ≥99.0%), N,N-dibutylethanolamine (DBAE, 99%) and ammonium hydroxide solution (28.0–30.0 wt%) are purchased from Sigma-Aldrich. Hexadecyl trimethyl ammonium bromide (CTAB, 99%) and catechol (Cat, 99%) are purchased from Aladdin. Hydrochloric acid (36.0–38.0 wt%), sodium chloride (NaCl), sodium phosphate dibasic dodecahydrate (Na2HPO4·12H2O), sodium dihydrogen phosphate dihydrate (NaH2PO4·2H2O), ethanol (≥99.7%) and acetone (≥99.5%) are obtained from Sinopharm Chemical Reagent Co., Ltd. Ultrapure water (>18.2 MΩ cm−1) is prepared by Millipore (Milford, MA, USA). ITO glass (square resistance < 17 Ω sq−1) is obtained from Kaivo Electronic Components Co., Ltd.
The electrochemical workstation (CHI 660E) is from Shanghai CH Instrument Co., Ltd. Transmission electron microscope (TEM, TecnaiG2 F20) is conducted with a TecnaiG2-F20 transmission electron microscope. Scanning electron microscope (SEM, SU8010) is performed with a Hitachi-SU8010 scanning electron microscope. Ultra-thin copper grid is obtained from Beijing Zhongke Building Electronic Technology Co., Ltd.
The electrochemical workstation (CHI 660E) is from Shanghai CH Instrument Co., Ltd. Transmission electron microscope (TEM, TecnaiG2 F20) is conducted with a TecnaiG2-F20 transmission electron microscope. Scanning electron microscope (SEM, SU8010) is performed with a Hitachi-SU8010 scanning electron microscope. Ultra-thin copper grid is obtained from Beijing Zhongke Building Electronic Technology Co., Ltd.
7
Silk Fibroin-based Hydrogel Synthesis
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The cocoon shell of Bombyx Mori silkworm was purchased from Chihe town, Shaanxi, China. Gelatin type A (300 bloom from porcine skin) was purchased from Merck Co., Ltd. Sodium phosphate dibasic dodecahydrate (Na2HPO4·12H2O) and sodium hydroxide (NaOH) were purchased from Sinopharm Co., Ltd. Calcium chloride anhydrous (CaCl2) and sodium carbonate (Na2CO3) were obtained from Macklin Co., Ltd. Methacrylic anhydride (MA) was purchased from Sigma-Aldrich Co., Ltd. Irgacure 2959 was obtained from BASF Co., Ltd. All chemicals and solvents purchased were used as received without further purification.
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