Thirty paraffin-embedded breast cancer tissue samples, four fresh breast cancer, and normal adjacent tissues were collected from The Affiliated Cancer Hospital of Zhengzhou University from October 2016 to October 2018. Written informed consent from all patients and approval of the hospital ethics review committees were obtained. Human embryonic kidney HEK293T cells and breast cancer cell lines MCF-7 and MDA-MB-231 were obtained from the ATCC (Manassas, VA, USA) and preserved in our laboratory. Adriamycin-resistant MCF-7 cells (MCF-7/Adr) were purchased from KeyGen BioTECH (Nanjing, China). MCF-7 and HEK293T cells were cultured in high-glucose Dulbecco’s modified Eagle’s medium (GIBCO). MDA-MB-231 cells were maintained in L15 medium (GIBCO), and MCF-7/Adr cells were cultured in 1640 medium (GIBCO). All the media were supplemented with 10% fetal bovine serum (GIBCO), 80 U/mL penicillin, and 0.08 mg/mL streptomycin. Cells were incubated at 5% CO2 at 37°C. Trichostatin A (TSA), doxorubicin hydrochloride (Adr), and Paclitaxel (taxol) were purchased from Medchemexpress (Monmouth Junction, NJ, USA).
Trichostatin a tsa
Manufactured by MedChemExpress
Sourced in United States, China
Trichostatin A (TSA) is a histone deacetylase (HDAC) inhibitor. It acts by inhibiting the activity of HDAC enzymes, which are responsible for the deacetylation of histones, leading to the accumulation of acetylated histones in cells. This can result in changes in gene expression and cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
L 15 medium, by Thermo Fisher Scientific (1 mentions)
Mda mb 231, by Thermo Fisher Scientific (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
1640 medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Mcf 7, by Thermo Fisher Scientific (1 mentions)
Mcf 7 adr cells, by Thermo Fisher Scientific (1 mentions)
Mcf 7 adr, by Keygen Biotech (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Protease inhibitor cocktail tablet, by Merck Group (1 mentions)
Bca protein quantification kit, by Beyotime (1 mentions)
Fastquant rt kit, by Tiangen Biotech (1 mentions)
Iodoacetamide, by Merck Group (1 mentions)
Triton x 100, by Sangon (1 mentions)
Acetonitrile acn, by Thermo Fisher Scientific (1 mentions)
Anti acetyl lysine antibody beads, by PTM Biolabs (1 mentions)
Formic acid, by Merck Group (1 mentions)
Trypsin, by Promega (1 mentions)
4 protocols using trichostatin a tsa
1
Breast Cancer Cell Line Cultivation
2
Quantitative Proteomics of SIRT2 Acetylation
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Antibody against GAPDH and SIRT2 was purchased from Abcam. Triton X-100 was bought in Sangon Biotech. Protease Inhibitor Cocktail tablets were purchased from Merck Millipore. Trichostatin A (TSA) was bought in MedChemExpress. Nicotinamide (NAM), trichloroacetic acid (TCA), triethylammonium bicarbonate (TEAB), DTT, iodoacetamide, EDTA, 2-deoxy-glucose (2-DG), TFA, and formic acid (FA) were bought from Sigma-Aldrich. BCA protein quantification kit was bought from Beyotime Biotechnology. Trypsin was purchased from Promega. Anti-acetyl-lysine antibody beads were bought from PTM BIO. Acetonitrile (ACN) was purchased from ThermoFisher Scientific. TRIzol reagent was bought in Invitrogen. FastQuant RT kit was bought from TianGen. Oligomycin was bought from Toronto Research Chemicals.
3
Protein Expression and Immunoblotting Assays
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Protein extraction and western blotting were performed as described in our previous research.16 Antibodies against NCoA6 (1:1000), E‐cadherin (1:5000), N‐cadherin (1:3000), FBW7 (1:1000), CDX2 (1:1000), cyclin‐dependent kinase 4 (CDK4, 1:2000), cyclin‐dependent kinase 2 (CDK2, 1:5000), Cyclin D1 (1:5000), Cyclin E1 (1:1000), and Cyclin A2 (1:2000) were obtained from Proteintech. Antibodies against p21 and p27 (1:1000) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against HDAC1, Vimentin, and AcH4 (1:1000) were purchased from Abcam. The AcH3 antibodies (1:1000) and β‐actin (1:10000) were produced by Abclonal. All secondary antibodies were purchased from Abclonal. Trichostatin A (TSA), an HDAC1 inhibitor, was purchased from MedChem Express.
4
Histone Deacetylase Inhibitors Modulate Cell Signaling
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Vorinostat (SAHA) and Trichostatin A (TSA) were purchased from MedChem Express (Shanghai, China). Anti-GFAP (sc-33673) was purchased from Santa Cruz (Dallas, TX, USA). Anti-α-SMA (A5228), Anti-Desmin (D1033), Anti-acetyl-Histone H3 (H9286), Anti-acetyl-Histone H4 (SAB5600021) and Anti-actin (A5060) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-Cleaved Caspase-9 (9507), Anti-Cleaved Caspase-3 (5A1E), Anti-Smad5 (12534) were purchased from Cell Signaling (Beverly, MA, USA). Anti-Smad7 (42-0400) and Bcl-2 (13-8800) were purchased from Invitrogen (Carlsbad, CA, USA). RT reagent kit and Premix Ex Taq were from TAKARA (Dalian, China). Lipofectamine 2000 was purchased from Invitrogen (Carlsbad, CA, USA). Luciferase kit was purchased from Promega (WI, USA). Apoptosis kit was purchased from BD Biosciences (San Jose, CA, USA).
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