Mega mouse universal genotyping array megamuga

DNA was extracted and purified from tail biopsies taken from 6-wk-old mice using QIAGEN DNeasy kit according to the manufacturer’s instructions. Genotyping was performed using the Mega Mouse Universal Genotyping Array (MegaMUGA) by GeneSeek (Neogen, Lansing, MI) (Welsh et al. 2012 (link)). The MegaMUGA array is built on the Illumina Infinium platform and contains 77,800 SNP markers that are distributed throughout the genome at an average spacing of 33 kb. For the mapping, genomes were reconstructed based on the X and Y allele intensities from the array and founder haplotypes were reconstructed using a hidden Markov model. The reconstructed haplotypes were then used to perform linkage mapping.

DNA was extracted and purified from tail biopsies taken from 6-week-old mice using the QIAGEN DNeasy kit, following the manufacturer’s instructions. Genotyping was performed using the Mega Mouse Universal Genotyping Array (MegaMUGA) by GeneSeek (Neogen, Lincoln, NE, USA) (Svenson et al. 2012 (link)).

DNA was extracted and purified from tail samples using Qiagen DNeasy kit (Qiagen, MD, USA) according to the manufacturer’s instructions. Genotyping was performed using the Mega Mouse Universal Genotyping Array (MegaMUGA) by GeneSeek (Neogen, Lansing, MI) (Welsh et al. 2012 (link)). The MegaMUGA array is built on the Illumina Infinium platform and contains 77,800 SNP markers that are distributed throughout the genome at an average spacing of 33 Kb. Genotypes of DO mice are accessible through UNC’s Mutant Mouse Resource and Research Centers website (https://www.med.unc.edu/mmrrc/genotypes/). We estimated heritability (h²) from allele probability using a linear mixed model in R package QTL2 version 0.18 (Broman et al. 2019 (link)).