Time-lapse images of control and KDM1A depleted hESCs were captured using an optical microscope (JuLI™ Stage Real-Time Cell History Recorder, NanoEntek) equipped with a high-sensitivity monochrome CCD (Sony sensor 2/3”), an automated x-y-z stage, and a 0.3 NA/10X objective (Olympus). hESCs were plated on vitronectin in presence of E8 Medium and recorded for 72 h at 60-min interval. The growth curve rate was automatically calculated on thresholded images, by measuring the total area occupied by the whole cells using the JuLI Stage software (NanoEntek).
For real-time imaging of beating cardiomyocytes, cells were recorded with the EVOS-FL Auto 2 Cell Imaging System (Thermo Fisher Scientific). During the acquisition cells were incubated at 37°C and 5% CO2 in a controlled stage incubator. Movies were acquired with an EVOS™10 × 0.30NA/7.13WD objective (Thermo Fisher Scientific).
For real-time imaging of beating cardiomyocytes, cells were recorded with the EVOS-FL Auto 2 Cell Imaging System (Thermo Fisher Scientific). During the acquisition cells were incubated at 37°C and 5% CO2 in a controlled stage incubator. Movies were acquired with an EVOS™10 × 0.30NA/7.13WD objective (Thermo Fisher Scientific).