All tomographic data were collected in electron-counting mode using SerialEM software version 3.8 (Mastronarde, 2003 (link)) on a Titan Krios G3i (Thermo Fisher Scientific) equipped with a Gatan energy filter and a K3 electron detector (Gatan). Data were recorded on a Ted Pella waffle pattern grating replica with 2160 lines/mm on a 3mm grid (Ted Pella), at a pixel size of 0.68Å and a target defocus of −1 μm.
Waffle pattern grating replica
Manufactured by Ted Pella
The Waffle pattern grating replica is a laboratory equipment product offered by Ted Pella. It serves as a replica of a waffle-patterned grating. The core function of this product is to provide a sample or reference material for various scientific and analytical applications.
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Lab products found in correlation
2 protocols using waffle pattern grating replica
1
Titan Krios G3i Tomographic Data Collection
2
Cryo-EM Data Collection on Titan Krios
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All tomographic data were collected in electron-counting mode using SerialEM software version 3.8 (Mastronarde, 2003) (link) on a Titan Krios G3i (Thermo Fisher Scientific) equipped with a Gatan energy filter and a K3 electron detector (Gatan). Data were recorded on a Ted Pella waffle pattern grating replica with 2160 lines/mm on 3mm grid (Ted Pella), at a pixel size of 0.68Å and a defocus of -1 µm.
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