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Spc 186c

Manufactured by Merck Group
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The SPC-186C is a laboratory equipment product manufactured by Merck Group. It is designed to perform specific functions within a laboratory setting. The core function of this equipment is to provide precise measurements and data collection capabilities for research and analysis purposes. Further details about its intended use or specific applications are not available within the scope of this factual and unbiased description.

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Lab products found in correlation

Restore stripping buffer, by Thermo Fisher Scientific (2 mentions) Hrp conjugated goat anti rat igg, by Thermo Fisher Scientific (2 mentions)

2 protocols using spc 186c

1

Extracting and Analyzing Parasite Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
To obtain total parasite protein extracts, infected erythrocytes were lysed with cold 0.1% saponin and washed with cold PBS containing protease inhibitors (Roche #11873580001). Isolated parasites were directly resuspended in SDS-PAGE loading buffer, boiled for 5 min at 95ºC and stored at −80ºC. Before performing SDS-PAGE, β-mercaphotetanol was added to a final concentration of 4% and samples boiled again for 5 min at 95ºC. Protein extracts were separated on 8% SDS-polyacrylamide gels and transferred to nitrocellulose membranes following standard procedures. We used a rat anti-HA monoclonal antibody (Roche #11867423001) at 1:200 and as a secondary antibody an HRP conjugated goat-anti-rat IgG (Thermo Fisher #A10549) at 1:1,000. To control for equal loading of parasite material between different samples, membranes were stripped with Restore stripping buffer (Thermo Scientific) according to the manufacturer’s instructions and re-probed with an antibody against PfHSP70 (1:10,000; StressMarq Biosciences #SPC-186C, lot #1007) and an HRP conjugated goat-anti-rabbit IgG (Sigma #A6154) secondary antibody at 1:5,000.
Bancells C., Llorà-Batlle O., Poran A., Nötzel C., Rovira-Graells N., Elemento O., Kafsack B.F, & Cortés A. (2018). Revisiting the initial steps of sexual development in the malaria parasite Plasmodium falciparum. Nature microbiology, 4(1), 144-154.
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2

Extracting and Analyzing Parasite Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
To obtain total parasite protein extracts, infected erythrocytes were lysed with cold 0.1% saponin and washed with cold PBS containing protease inhibitors (Roche #11873580001). Isolated parasites were directly resuspended in SDS-PAGE loading buffer, boiled for 5 min at 95ºC and stored at −80ºC. Before performing SDS-PAGE, β-mercaphotetanol was added to a final concentration of 4% and samples boiled again for 5 min at 95ºC. Protein extracts were separated on 8% SDS-polyacrylamide gels and transferred to nitrocellulose membranes following standard procedures. We used a rat anti-HA monoclonal antibody (Roche #11867423001) at 1:200 and as a secondary antibody an HRP conjugated goat-anti-rat IgG (Thermo Fisher #A10549) at 1:1,000. To control for equal loading of parasite material between different samples, membranes were stripped with Restore stripping buffer (Thermo Scientific) according to the manufacturer’s instructions and re-probed with an antibody against PfHSP70 (1:10,000; StressMarq Biosciences #SPC-186C, lot #1007) and an HRP conjugated goat-anti-rabbit IgG (Sigma #A6154) secondary antibody at 1:5,000.
Bancells C., Llorà-Batlle O., Poran A., Nötzel C., Rovira-Graells N., Elemento O., Kafsack B.F, & Cortés A. (2018). Revisiting the initial steps of sexual development in the malaria parasite Plasmodium falciparum. Nature microbiology, 4(1), 144-154.
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