All mouse experiments were carried out with strict observance of protocols and guidelines approved by the University of Bonn Animal Care and Use Committee, Federal Government of Germany and European Union legislation. The protocols were approved by the Landesamt für Natur, Umwelt und Verbraucherschutz Nordrhein-Westfalen (Permit Number: 84-02.02.2016.A238). The mice were housed under controlled light (12 h light/dark cycle at an ambient temperature of 22°C). Water and mice chow were available ad libitum.
Cxcr4CreER(T2)-IRES-eGFP(Cxcr4CreER) mice were provided by Ralf Stumm (Werner et al., 2020 (link)). Rosa26Ai9 (stock #007909; Madisen et al., 2010 (link)), DattTA (stock #027178; Chen et al., 2015 (link)), and Ai82D (lgs7TITLGFP; stock #023532; Madisen et al., 2015 (link)) mice were purchased from The Jackson Laboratory. For inducible fate-mapping studies, Cxcr4CreER/+; Rosa26Ai9/Ai9 males were bred with CD1 wild-type females (Charles River) to generate Cxcr4CreER/+; Rosa26Ai9/+ progeny. For intersectional fate mapping, Cxcr4CreER/+; DattTA/+; lgs7TITLGFP/TITLGFP males were bred with CD1 wild-type females (Charles River) to generate Cxcr4CreER/+; DattTA/+; lgs7TITLGFP progeny. Mice of either sex were used for experiments.
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