Cxcr4CreER(T2)-IRES-eGFP(Cxcr4CreER) mice were provided by Ralf Stumm (Werner et al., 2020 (link)). Rosa26Ai9 (stock #007909; Madisen et al., 2010 (link)), DattTA (stock #027178; Chen et al., 2015 (link)), and Ai82D (lgs7TITLGFP; stock #023532; Madisen et al., 2015 (link)) mice were purchased from The Jackson Laboratory. For inducible fate-mapping studies, Cxcr4CreER/+; Rosa26Ai9/Ai9 males were bred with CD1 wild-type females (Charles River) to generate Cxcr4CreER/+; Rosa26Ai9/+ progeny. For intersectional fate mapping, Cxcr4CreER/+; DattTA/+; lgs7TITLGFP/TITLGFP males were bred with CD1 wild-type females (Charles River) to generate Cxcr4CreER/+; DattTA/+; lgs7TITLGFP progeny. Mice of either sex were used for experiments.
Rosa26ai9
The Rosa26Ai9 is a genetically engineered mouse model that expresses the Ai9 fluorescent reporter gene under the control of the ubiquitously expressed Rosa26 promoter. The Ai9 gene encodes a red fluorescent protein that can be used to visualize and track cells in vivo.
1 protocol using rosa26ai9
Inducible Fate Mapping of CXCR4+ Cells
Cxcr4CreER(T2)-IRES-eGFP(Cxcr4CreER) mice were provided by Ralf Stumm (Werner et al., 2020 (link)). Rosa26Ai9 (stock #007909; Madisen et al., 2010 (link)), DattTA (stock #027178; Chen et al., 2015 (link)), and Ai82D (lgs7TITLGFP; stock #023532; Madisen et al., 2015 (link)) mice were purchased from The Jackson Laboratory. For inducible fate-mapping studies, Cxcr4CreER/+; Rosa26Ai9/Ai9 males were bred with CD1 wild-type females (Charles River) to generate Cxcr4CreER/+; Rosa26Ai9/+ progeny. For intersectional fate mapping, Cxcr4CreER/+; DattTA/+; lgs7TITLGFP/TITLGFP males were bred with CD1 wild-type females (Charles River) to generate Cxcr4CreER/+; DattTA/+; lgs7TITLGFP progeny. Mice of either sex were used for experiments.
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