Cerulenin

Manufactured by Enzo Life Sciences

Cerulenin is a natural product isolated from the fungus Acremonium caerulens. It is a potent inhibitor of fatty acid synthase, a key enzyme involved in the biosynthesis of fatty acids.

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Lab products found in correlation

4 protocols using cerulenin

Lipid Metabolism Regulation in NF2 Cells

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pBabe-NF2 was obtained from Addgene. Anti-Fasn siRNA (M-040091-01-0005), anti-Acaca siRNA (M-063938-01-0005), anti-Mtor (M-065427-00-0005), anti-Rptor (M-058754-01-0005), anti-Rictor (M-064598-01-0005) and non-silencing (D-001206-13-05) siRNA were purchased from Dharmacon. Individual siRNAs against Mtor (SASI_Mm01-00164496 and -00164492), Rictor (-00137732 and -00137730), Rptor (-00055298 and -00334580), Fasn (-00177858 and -00177854), Acaca (-0011590 and -00115905), and Mlycd (-00028572 and -00028576) were purchased from Sigma-Aldrich. Anti-Merlin antibodies were purchased from Abcam (#ab88957). Lipid synthesis and metabolism antibody kit (includes anti-Fasn, -phospho ACC, -ACC, -Lipin1, -ACLY, -phospho ACLY, -ACSL1, and -ACECS1 antibodies), and anti-Casp3 antibodies were purchased from Cell Signaling Technology. Anti-SREBP1 antibodies were purchased from Santa Cruz Biotechnology. Anti-GAPDH antibodies were purchased from EMD-Millipore.
Cerulenin, C75, luteolin, 5-(tetradecyloxy)-2-furoic acid (TOFA) and 5-iodotubercidin were purchased from Enzo Life Sciences. GSK2194069, dimethylsulfoxyde (DMSO), staurosporin, sodium palmitate, 70% perchloric acid, ammonium formate, acetonitrile, acetyl-coenzyme A lithium salt, malonyl coenzyme A lithium salt, propionyl-coenzyme A lithium salt, and poly-L-lysine were purchased from Sigma-Aldrich.

Stepanova D.S., Semenova G., Kuo Y.M., Andrews A.J., Ammoun S., Hanemann C.O, & Chernoff J. (2017). An essential role for the tumor suppressor Merlin in regulating fatty acid synthesis. Cancer research, 77(18), 5026-5038.

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Modulating Yeast Lipid Metabolism

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Yeast strains were cultured either in YP medium [1% bacto yeast extract, 2% bacto peptone (United States Biological, Swampscott, MA)] or in synthetic complete (SC) medium [0.67% yeast nitrogen base without amino acids (United States Biological), 0.73 g/L amino acids], containing either 2% glucose or 2% galactose. Fatty acid-supplemented medium contained 0.24% Tween 40 (Sigma-Aldrich, St Louis, MO) and 0.12% oleic acid (Carl Roth, Karlsruhe, Germany), but no glucose. Synthesis of fatty acids and sterols was inhibited by the addition of cerulenin (10 μg/mL; Enzo Life Sciences, Inc., Farmingdale, NY) and terbinafine (30 μg/mL; Sigma-Aldrich, St Louis, MO). Expression of the membrane-proximal PLIN3 constructs was repressed by cultivating cells in the presence of doxycycline (1 μg/mL; Sigma-Aldrich, St Louis, MO) and expression was induced by shifting cells to media lacking doxycycline, typically 14–16 h before imaging.

Khaddaj R., Stribny J., Cottier S, & Schneiter R. (2023). Perilipin 3 promotes the formation of membrane domains enriched in diacylglycerol and lipid droplet biogenesis proteins. Frontiers in Cell and Developmental Biology, 11, 1116491.

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Modulating Mitochondrial Dynamics in Drosophila

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1-d-old adult flies were placed on molasses medium supplemented with 100 µM Cerulenin (Enzo Life Sciences), 3 µM Irgasan (Sigma-Aldrich), or 100 µM Orlistat (Sigma-Aldrich), unless otherwise indicated in Fig. 2 (B, D, and F; 2.5% final ethanol concentration), or flies were supplemented with 2.5% ethanol for control medium. The animals were kept on these media for 72 h and tested for flight or ATP levels. To determine mitochondrial morphology, embryos were placed in molasses medium supplemented with 100 µM Cerulenin (2.5% final ethanol concentration) or with 2.5% ethanol for control medium and grown to the third instar larval stage. These third instar larvae were dissected and used for experiments (see Determination of mitochondrial morphology).
To assess ATP levels in the cells, cells were treated with 10 µM Cerulenin for 24 h (0.1% ethanol) or with control medium supplemented with 0.1% ethanol. For Western blotting analysis, wild-type MEFs were treated with 100 µM H₂O₂ for 30 min.

Vos M., Geens A., Böhm C., Deaulmerie L., Swerts J., Rossi M., Craessaerts K., Leites E.P., Seibler P., Rakovic A., Lohnau T., De Strooper B., Fendt S.M., Morais V.A., Klein C, & Verstreken P. (2017). Cardiolipin promotes electron transport between ubiquinone and complex I to rescue PINK1 deficiency. The Journal of Cell Biology, 216(3), 695-708.

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Cerulenin inhibition of Xanthomonas campestris

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Cerulenin was purchased from Enzo Life Sciences and was dissolved in ethanol for a stock solution (3 mg ml⁻¹). Xcc strains were grown overnight in NA liquid medium at 37 °C. Cell pellets were then collected by centrifugation at 6,000 × g for 15 min. After washing twice using fresh NA liquid medium, pellets were re-suspended in NA medium to an OD₆₀₀ of 1.0. Cerulenin was added to the culture at a final concentration ranging from 20 to 60 μg ml⁻¹. After further growth for 3 h and 6 h, 10 ml of cell culture were collected for DSF and BDSF extraction.

Zhou L., Yu Y., Chen X., Diab A.A., Ruan L., He J., Wang H, & He Y.W. (2015). The Multiple DSF-family QS Signals are Synthesized from Carbohydrate and Branched-chain Amino Acids via the FAS Elongation Cycle. Scientific Reports, 5, 13294.

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