The antioxidant capacity was investigated by a DPPH• radical scavenging in vitro assay [41 (link)]. The activity of the standard or EE was determined by adding 100 μL of ethanol 95% (v/v) (Vetec, Recife, Brazil), 100 μL of sodium acetate buffer 100 mM (pH 5.5, Merck,, Darmstadt, Germany), 50 μL of 507.2 μM DPPH (Sigma-Aldrich, Jurubatuba, Brazil) radical solution in ethanol, and 10 μL of standard or sample to 96-well plate. After 15 min, the spectrophotometer was read, discounting the blank. For the blank, 150 μL of ethanol 95% (v/v), 100 μL of sodium acetate buffer (pH 5.5), and 10 μL of standard or sample were added in the same concentration. The absorbance was determined at 517 nm using a Multimode Plate Reader (EnSpire, Perkin-Elmer, Singapore). Ascorbic acid (AA, Merck, Darmstadt, Germany) was used as positive control (0.6–6 µg/mL). The results were expressed as the efficient concentration that can scavenge DPPH• radical at 50% (EC50). All analyses were performed in triplicate, and data were expressed as mean ± standard deviation.
Ethanol
Manufactured by Vetec
Sourced in Brazil, United States
Ethanol is a clear, colorless liquid that serves as a versatile laboratory reagent. It is commonly used as a solvent, disinfectant, and fuel source in various scientific applications.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Vetec (7 mentions)
Sodium hydroxide (naoh), by Vetec (5 mentions)
Acetone, by Vetec (5 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (5 mentions)
Dimethyl sulfoxide (dmso), by Vetec (4 mentions)
Gallic acid, by Merck Group (3 mentions)
Dexamethasone (dex), by Merck Group (3 mentions)
Quercetin, by Merck Group (3 mentions)
Acetic acid, by Vetec (3 mentions)
Chloroform, by Vetec (3 mentions)
Hydrochloric acid (hcl), by Vetec (3 mentions)
Potassium persulfate, by Merck Group (2 mentions)
2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid, by Merck Group (2 mentions)
Milli q purification system, by Merck Group (2 mentions)
Trolox, by Merck Group (2 mentions)
Tween 80, by Merck Group (2 mentions)
N hexane, by Vetec (2 mentions)
Fetal bovine serum (fbs), by Cultilab (2 mentions)
Penicillin streptomycin, by Merck Group (2 mentions)
Dichloromethane, by Vetec (2 mentions)
33 protocols using ethanol
1
Antioxidant Capacity Analysis via DPPH Radical Scavenging
2
Pulp Delignification Reagents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The reagents used were glacial acetic acid (> 99.85%, Sigma-Aldric), sulfuric acid (> 96%, Vetec), ethanol (> 95% vol, Vetec), sodium chlorite (> 80%, Sigma-Aldrich) and sodium hydroxide P.A (98%, Merck).
3
Antioxidant and Cytotoxicity Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents used in this study were classified as of analytical purity grade: citric acid, phosphate-buffered saline (PBS), dimethyl sulfoxide (DMSO), 1% agarose gel, ethanol and methanol, purchased from Vetec (Duque de Caxias, RJ, Brazil); sodium carbonate; Folin-Ciocalteau reagent, purchased from Êxodo Científica (Hortolândia, SP, Brazil); 2,2'azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), potassium persulfate, gallic acid, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox), gentamicin sulfate and fetal bovine serum (FBS), purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA); Dulbecco's Modified Eagle's medium (DMEM), sodium bicarbonate, penicillin and streptomycin, purchased from Gibco (Grand Island, NY); (3) (4) -2,5-diphenyl tetrazolium] bromide (MTT) purchased from Invitrogen, Thermo Fisher Scientific (Oregon, USA). The aqueous solutions were prepared with ultrapure water (18.2 MΩ•cm resistivity at 25 °C), obtained by the Milli-Q deionization system (Millipore, Bedford, MA, USA).
4
Antioxidant and Fuel Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
High-purity deionized water (R ≥ 18 MΩ cm) obtained from a Milli Q water purification system (Millipore, Bedford, MA, USA) was used for preparing all aqueous solutions. Concentrated perchloric acid (70%, m/v) and ethanol (99.9%, v/v) were obtained from Vetec (Rio de Janeiro, Brazil). The antioxidant 2,6-tert-butylphenol (2,6-DTBP), ferrocene (99%) and tetrabutylammonium perchlorate (TBAClO4, for electrochemical analysis) were obtained from Aldrich (USA). Working standard solutions were prepared immediately before use by appropriate dilution of the stock solution. A standard stock solution containing 2,6-DTBP (1x10 -2 mol L -1 ) was prepared in ethanol containing 0.1 mol L -1 HClO4. All reagents were used without further purification (analytical grade). Samples of aviation jet fuel were donated by the Radio Patrol Air Battalion (Brazilian Air Force), located at Uberlândia Minas Gerais, Brazil.
Biodiesel samples were produced in laboratory from fat pork, coconut oil (Cocos nucifera) and Macauba oil (Acrocomia aculeate) through alkaline transesterification.
Biodiesel samples were produced in laboratory from fat pork, coconut oil (Cocos nucifera) and Macauba oil (Acrocomia aculeate) through alkaline transesterification.
5
Acid-Base Titration Procedure
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hydrochloric acid (Synth, Diadema, São Paulo, Brazil), sodium hydroxide (Vetec, Duque de Caxias, Rio de Janeiro, Brazil), potassium hydrogen phthalate (Synth, Diadema, São Paulo, Brazil), ethanol (Vetec, Duque de Caxias, Rio de Janeiro, Brazil), sodium tetraborate (Synth, Diadema, São Paulo, Brazil), phenolphthalein (Synth, Diadema, São Paulo, Brazil), and methyl orange (Isofar, Duque de Caxias, Rio de Janeiro, Brazil) were used without any purification. Filter paper model JP40 was obtained from Quanty (São José dos Pinhais, Paraná, Brazil).
6
Vascular Function and Oxidative Stress Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Phenylephrine hydrochloride (Sigma, cod. P6126), Acetylcholine chloride (Sigma, cod. A6625), Nω-Nitro-L-arginine methyl ester hydrochloride (Sigma, cod. N5751), charybdotoxin (Sigma, cod. C7802), indomethacin (Sigma, cod. I7378), apamin (Sigma, cod. A1289), Dihydroethidium (Sigma, cod. D7008), 2,2-Diphenyl-1-picrylhydrazyl (Sigma, cod. D9132), Folin-Ciocalteu’s (Sigma, cod. 47,641), ascorbic acid, gallic acid, from Sigma-Aldrich®, 4′,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI) from Invitrogen Molecular Probes™ (cod. D1306) and Dako Fluorescence Mounting Medium (cod. S3023) Na2CO3, NaCl, KCl, KH2PO4, NaHCO3, C6H12O6, CaCl2, MgSO4, ethylenediaminetetraacetic Acid (EDTA), ethanol, methanol, from Vetec®.
7
Synthesis of Titanium-Niobium Oxide
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The tetraethylorthosilicate (TEOS, 98%), titanium (IV) butoxide (97%) and niobium (V) pentachloride (99%) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Ethanol (99.8%), HCl (37% v/v) and HNO3 (95% v/v) were purchased from Vetec (Duque de Caxias, RJ, Brazil). Ultra-pure Milli-Q water was used (resistivity > 18.2 MΩ cm−1, 25 °C, Millipore Milli-Q purification system, Billerica, MA, USA).
8
Characterization of Coumarins' Physicochemical Properties
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The solvents acetonitrile and formic acid were obtained from Tedia (HPLC grade, Radnor, PA, USA), and ultrapure water was obtained from the Milli-Q® Plus apparatus by Millipore (Billerica, MA, USA). Methanol, ethanol, isopropanol, polysorbate 80 (Tween® 80), dimethyl sulfoxide (DMSO), and sodium hydroxide were obtained from Vetec (Duque de Caxias, Brazil), and medium chain triglycerides (MCT) and egg lecithin (Lipoid® E80) were purchased from Lipoid GmbH (Ludwigshafen am Rhein, Germany). Monobasic potassium phosphate was obtained from Dinâmica (São Paulo, Brazil), and NBD-PE [N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt] fluorescent-labelled phospholipid was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Roswell Park Memorial Institute 1640 broth medium (RPMI-1640), 3(N-morpholino) propane sulfonic acid (MOPS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and itraconazole were obtained from Sigma-Aldrich (St. Louis, MA, USA), and potato dextrose agar (PDA) was purchased from Acumedia (San Bernardino, CA, USA). Tissue-Tek® O.C.T.™ was purchased from Sakura Finetechnical Co. (Tokyo, Japan). Log P (calculated log P) values of the coumarins were assigned using SwissADME http://www.swissadme.ch/ (accessed on 10 March 2023).
9
Jaboticaba Fruit Extraction and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Jaboticaba (Myrciaria cauliflora Berg.) was purchased in a local market (Rio de Janeiro, RJ, Brazil).
For extractions, ethanol (Vetec, Rio de Janeiro, Brazil) and distilled water were used. For analysis of phenolic compounds and antioxidant capacity, Folin–Ciocalteau reagent, ABTS+, K2SO5, and Trolox were purchased from Sigma-Aldrich (St. Louis, MO, USA). We also acquired sodium bicarbonate (Alphatec, Macaé, RJ, Brazil), formic acid (Merck, Rahway, NJ, USA), and methanol (Tedia, Fairfield, CA, USA). Standard of the 3-O-glucoside of cyanidin was isolated from natural source, with purities greater than 99%.
For extractions, ethanol (Vetec, Rio de Janeiro, Brazil) and distilled water were used. For analysis of phenolic compounds and antioxidant capacity, Folin–Ciocalteau reagent, ABTS+, K2SO5, and Trolox were purchased from Sigma-Aldrich (St. Louis, MO, USA). We also acquired sodium bicarbonate (Alphatec, Macaé, RJ, Brazil), formic acid (Merck, Rahway, NJ, USA), and methanol (Tedia, Fairfield, CA, USA). Standard of the 3-O-glucoside of cyanidin was isolated from natural source, with purities greater than 99%.
10
Histological Analysis of Thyroid Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The thyroids were collected and fixed in 10% formaldehyde (pH 7.4) for 48 h, followed by dehydration in ethanol (Vetec, Rio de Janeiro, Brazil) and clarification in xylene (Vetec, Rio de Janeiro, Brazil). Tissues were embedded in paraffin and cut at the thickness of 5 μm. Sections were stained with hematoxylin and eosin for topographic analysis according to Andrade et al. (27 (link)). High-resolution images (1600 × 1200 pixels) were obtained using digital camera (QImaging Retigar-2000R Fast 1394 Mono, Surrey BC, Canada) coupled to light microscope (Olympus BX50). High-quality images were captured with the QCapture Pro7 (QImaging).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!