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P44 42 mapk erk1 2

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The P44/42 MAPK (ERK1/2) is a laboratory equipment used for the detection and quantification of two isoforms of mitogen-activated protein kinase (MAPK), also known as extracellular signal-regulated kinases 1 and 2 (ERK1/2). This equipment is designed to facilitate the analysis of cellular signaling pathways and their regulation.

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Lab products found in correlation

Doxorubicin, by Merck Group (2 mentions) Chloroquine, by Merck Group (2 mentions) Cleaved parp, by Abcam (2 mentions) Phospho p44 42 mapk p erk, by Thermo Fisher Scientific (2 mentions) Atg12, by Santa Cruz Biotechnology (2 mentions) Abc232, by Merck Group (2 mentions) 6 aminonicotinamide, by Merck Group (2 mentions) 1 13c glucose, by Cambridge Isotopes (2 mentions) 1 14c glucose and 6 14c glucose, by PerkinElmer (2 mentions) Phospho histone h3, by Cell Signaling Technology (2 mentions) Quinacrine, by Merck Group (2 mentions) Hoechst, by Thermo Fisher Scientific (2 mentions) N acetylcysteine, by Merck Group (2 mentions) Etoposide, by Merck Group (2 mentions)

2 protocols using p44 42 mapk erk1 2

1

Immunoblotting and Immunofluorescence Analysis of Autophagy Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Commercial antibodies include: phospho-Histone H3 (Cell Signaling Technology (CST) 9701), cleaved caspase-3 Alexa Fluor 488 (CST 9669S), phospho-p44/42 MAPK (P-ERK) (Invitrogen 44–680G), p44/42 MAPK (ERK1/2) (Invitrogen 13–6200), ATG12 (CST 2010), ATG7 (Santa Cruz Biotechnology sc8668), p62 (Progen Biotechnik GP62C), tubulin (Sigma T6199), p53 (DO1, Calbiochem OP43), cleaved PARP (CST 9451), G6PD (Abcam ab993) LC3 5F10 for IF (Axxora NT0231-00). For immunobloting, we utilized an LC3 antibody which has been described previously and is now commercially available (EMD Millipore ABC232) (35 (link)). Chemicals include: chloroquine (CQ), quinacrine (Q), 6-aminonicotinamide (6-AN), N-acetyl Cysteine (NAC), etoposide (Et) and doxorubicin (DX), all from Sigma-Aldrich, staurosporine (STS, EMD Chemicals), 1-13C glucose (Cambridge Isotopes), 1-14C glucose and 6-14C glucose (Perkin Elmer), and Hoechst (Invitrogen).
Salas E., Roy S., Marsh T., Rubin B, & Debnath J. (2015). Oxidative Pentose Phosphate Pathway Inhibition Is A Key Determinant of Antimalarial Induced Cancer Cell Death. Oncogene, 35(22), 2913-2922.
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2

Immunoblotting and Immunofluorescence Analysis of Autophagy Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Commercial antibodies include: phospho-Histone H3 (Cell Signaling Technology (CST) 9701), cleaved caspase-3 Alexa Fluor 488 (CST 9669S), phospho-p44/42 MAPK (P-ERK) (Invitrogen 44–680G), p44/42 MAPK (ERK1/2) (Invitrogen 13–6200), ATG12 (CST 2010), ATG7 (Santa Cruz Biotechnology sc8668), p62 (Progen Biotechnik GP62C), tubulin (Sigma T6199), p53 (DO1, Calbiochem OP43), cleaved PARP (CST 9451), G6PD (Abcam ab993) LC3 5F10 for IF (Axxora NT0231-00). For immunobloting, we utilized an LC3 antibody which has been described previously and is now commercially available (EMD Millipore ABC232) (35 (link)). Chemicals include: chloroquine (CQ), quinacrine (Q), 6-aminonicotinamide (6-AN), N-acetyl Cysteine (NAC), etoposide (Et) and doxorubicin (DX), all from Sigma-Aldrich, staurosporine (STS, EMD Chemicals), 1-13C glucose (Cambridge Isotopes), 1-14C glucose and 6-14C glucose (Perkin Elmer), and Hoechst (Invitrogen).
Salas E., Roy S., Marsh T., Rubin B, & Debnath J. (2015). Oxidative Pentose Phosphate Pathway Inhibition Is A Key Determinant of Antimalarial Induced Cancer Cell Death. Oncogene, 35(22), 2913-2922.
+ Open protocol
+ Expand

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