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Ex taq qpcr premix

Manufactured by Roche
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Ex TAq qPCR Premix is a ready-to-use master mix for quantitative real-time PCR (qPCR) applications. It contains all the necessary components, including DNA polymerase, dNTPs, and buffer, to perform qPCR reactions.

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Lab products found in correlation

Real time pcr lightcycler 2, by Roche (6 mentions) M mlv, by Promega (6 mentions) Tri reagent, by Merck Group (5 mentions)

Close spelling variants of this product

Premix Ex Taq

6 protocols using ex taq qpcr premix

1

Quantitative Real-Time PCR Analysis

Cited 1 time
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Total RNA was extracted using TRI Reagent® (Sigma-Aldrich). Three μg of RNA was used for retro-transcription with M-MLV (Promega, Madison, WI). qPCR was performed in triplicates by using validated qPCR primers (BLAST), Ex TAq qPCR Premix, and the Real-Time PCR LightCycler II (Roche Diagnostics, Indianapolis, IN) as previously described40 (link). mRNA levels were normalized to actin mRNA, and the relative mRNA levels were determined by using the 2−ΔΔCt method.
Lettieri Barbato D., Tatulli G., Maria Cannata S., Bernardini S., Aquilano K, & Ciriolo M.R. (2015). Glutathione Decrement Drives Thermogenic Program In Adipose Cells. Scientific Reports, 5, 13091.
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2

Quantitative Analysis of OxPHOS Genes

Cited 1 time
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Total RNA was extracted using TRI Reagent® (Sigma-Aldrich). Three micrograms of RNA was used for retro-transcription with M-MLV (Promega, Madison, WI). qPCR was performed in triplicates by using validated qPCR primers (BLAST), Ex TAq qPCR Premix, and the Real-Time PCR LightCycler II (Roche Diagnostics, Indianapolis, IN) as previously described (Lettieri Barbato et al., 2013 (link)). mRNA levels were normalized to actin mRNA, and the relative mRNA levels were determined by using the 2−ΔΔCt method. To calculate OxPHOS gene expression ratio, nuclear-encoded OxPHOS mRNA levels were compared to mitochondrial-encoded OxPHOS mRNA. The relative mRNA levels were determined by using the 2−ΔΔCt method and were normalized to actin.
Lettieri Barbato D., Tatulli G., Vegliante R., Cannata S.M., Bernardini S., Ciriolo M.R, & Aquilano K. (2015). Dietary fat overload reprograms brown fat mitochondria. Frontiers in Physiology, 6, 272.
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3

RNA Extraction and qPCR Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using TRI Reagent® (Sigma-Aldrich). RNA (3 μg) was retro-transcripted by using M-MLV (Promega, Madison, WI). qPCR was performed in triplicate by using validated qPCR primers (BLAST), Ex TAq qPCR Premix, and the Real-Time PCR LightCycler II (Roche Diagnostics, Indianapolis, IN) as previously described [14 (link)]. mRNA levels were normalized to actin mRNA, and the relative mRNA levels were determined through the 2−ΔΔCt method.
Lettieri-Barbato D., Cannata S.M., Casagrande V., Ciriolo M.R, & Aquilano K. (2018). Time-controlled fasting prevents aging-like mitochondrial changes induced by persistent dietary fat overload in skeletal muscle. PLoS ONE, 13(5), e0195912.
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4

Quantitative Real-Time PCR Analysis

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Total RNA was extracted using TRI Reagent (Sigma-Aldrich) and used for retro-transcription as previously described [48 (link)]. Three micrograms of RNA was used for retro-transcription with M-MLV (Promega). qPCR was performed in triplicates by using validated qPCR primers (BLAST), Ex TAq qPCR Premix and the Real-Time PCR LightCycler II (Roche Diagnostics, Indianapolis, IN, USA). mRNA levels were normalized to RPL mRNA, and the relative mRNA levels were determined by using the 2−ΔΔCt method. The primer sequences are listed in Table 1.
Aquilano K., Baldelli S., Barbera L.L., Barbato D.L., Tatulli G, & Ciriolo M.R. (2016). Adipose triglyceride lipase decrement affects skeletal muscle homeostasis during aging through FAs-PPARα-PGC-1α antioxidant response. Oncotarget, 7(17), 23019-23032.
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5

RNA Extraction and qPCR Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using TRI Reagent® (Sigma-Aldrich). RNA (3 μg) was retro-transcripted by using M-MLV (Promega, Madison, WI). qPCR was performed in triplicate by using validated qPCR primers (BLAST), Ex TAq qPCR Premix, and the Real-Time PCR LightCycler II (Roche Diagnostics, Indianapolis, IN) as described by Lettieri Barbato et al. [42 (link)]. mRNA levels were normalized to actin mRNA, and the relative mRNA levels were determined through the 2−ΔΔCt method.
Lettieri-Barbato D., D’Angelo F., Sciarretta F., Tatulli G., Tortolici F., Ciriolo M.R, & Aquilano K. (2017). Maternal high calorie diet induces mitochondrial dysfunction and senescence phenotype in subcutaneous fat of newborn mice. Oncotarget, 8(48), 83407-83418.
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6

Quantitative Real-Time PCR Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using TRI Reagent. Three micrograms of RNA was used for retrotranscription with M-MLV (Promega). qPCR was performed in triplicates by using validated qPCR primers (BLAST), Ex TAq qPCR Premix and the Real-Time PCR LightCycler II (Roche Diagnostics, Indianapolis, IN, USA). mRNA levels were normalized to RPL mRNA, and the relative mRNA levels were determined by using the 2−ΔΔCt method. The primer sequences are listed in Table 1.
Baldelli S., Aquilano K, & Ciriolo M.R. (2014). PGC-1α buffers ROS-mediated removal of mitochondria during myogenesis. Cell Death & Disease, 5(11), e1515-.
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