α tubulin antibody t6074

The phospho-S6 antibody (Ser235/236, #2211), S6 antibody (#2317), and p110α antibody (#4255) were obtained from Cell Signaling Technology. p110δ antibodies (#sc-7176, mab2687) were obtained from Santa Cruz Biotechnology and R&D Biosciences, respectively. The p110β antibody (#09-482) was obtained from EMD Millipore, the α-tubulin antibody (T6074) was obtained from Sigma-Aldrich, and the anti-Puromycin antibody was purchased from the Developmental Studies Hybridoma Bank (University of Iowa). Secondary antibodies for Western blotting were obtained from VWR (horseradish peroxidase-coupled anti-mouse and anti-rabbit antibodies for ECL) or from Li-COR Biosciences (IRDye 680 RD donkey anti-mouse IgG and IRDye 800CW goat anti-rabbit IgG for infrared fluorescent Western blotting). Puromycin was purchased from Life Technologies, the p110δ inhibitor IC87114 was purchased from EMD Millipore, and the p110β inhibitor TGX-221 was purchased from Selleck Chemicals.

Cortisol, corticosterone, BSA, DAPI, α-tubulin antibody (T6074), melatonin (M5250), PD98059 (027K2176), 5-azacytidine (5-aza, A2385) and carbonyl cyanide m-chlorophenyl hydrazone (CCCP, C2759) were purchased from Sigma Chemical Company (St. Louis, MO, USA). Lamin A/C (sc-376248), β-actin (sc-47778), MT2 (sc-28453), dynein (sc-13524), NUP62 (sc-48373), importin β (sc-137016), BNIP3 (sc-56167), c-MYC (sc-40), IgG mouse (sc-2025), p-ERK (sc-7383), ERK (sc-94), JNK (sc-7345), p-JNK (sc-6254), p-p38 (sc-166182), and PINK1 (sc-33796) antibodies were acquired from Santa Cruz (Paso Robles, CA, USA). MT1 (NBP1-71113), FKBP4 (NB110-96874), FKBP5 (NBP1-84676), LC3 (NB100-2220) and NIX (NBP1-88558) were purchased from Novus Biologicals (Littleton, CO, USA). TOMM20 (ab56783), p-GR (ab55189), DNMT1 (ab19905), DNMT3a (ab23565), and SP1 (ab227383) antibodies were obtained from Abcam (Cambridge, MA, USA). Cleaved caspase 3 (9661 S), DNMT3b (67259 S), GR (12041 S), 5-methylcytosine (5-mc, 28692 S), p38 (9212 S), IgG rabbit (2729 S), and p-c-MYC ser62 (13748 S) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). HSP90 antibody (PA3-013) was obtained from Thermo Fisher (Rockford, IL, USA).

An antibody against PDGFRα (AF 1602) was purchased from R&D Systems (Minneapolis, MN, USA). The antibodies recognizing phospho-PDGFRα (pY849, #3170), phospho-PLCγ1 (pTyr783, #2821), PLCγ1 (#2822), phospho-STAT3 (pTyr705, D3A7, #9145), STAT3 (79D7, #4904), phospho-Akt (pSer473, D9E, #4060), Akt (#9272S), phospho-ERK1/2 (pThr202/pThr204, #9101) and ERK1/2 (137F5, #4695) were from Cell Signaling Technology (Danvers, MA, USA). Antibodies against SUMO1 (sc-5308) and ubiquitin (sc-8017) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The α-tubulin antibody (T6074) was from Sigma-Aldrich (St Louis, MO, USA). Primary antibodies against HA (NB600-363) and the transferrin receptor (TfR) (NB100-92243) were from Novus Biologicals (Centennial, CO, USA). Antisera recognizing Alix (HP95) [42 ] and PDGFRβ (CTβ) [43 ] were prepared in house. The secondary antibodies used for immunoblotting included goat anti-mouse IgG (62-6520), goat anti-rabbit IgG (65-6120) and rabbit anti-goat IgG (81-1620), were from Invitrogen (Waltham, MA, USA). The inhibitors MG132 (C2211) and chloroquine (C6628) were from Sigma-Aldrich (St Louis, MO, USA). The inhibitor ginkgolic acid (345,887) was purchased from Merck KGaA and bortezomib (#2244) was from Cell Signaling Technology (Danvers, MA, USA).