Ft ir 6100 spectrometer

The XRD analysis of each transparent film was performed on a MiniFlex II powder X-ray diffractometer (Rigaku, Tokyo, Japan) equipped with an Ni-filtered Cu-Kα radiation source (30 kV, 15 mA; scan rate: 1°/min; sampling step: 0.02°). Infrared spectroscopy studies were performed on each transparent film using the attenuated total reflection (ATR) method on an FT/IR 6100 spectrometer (JASCO, Tokyo, Japan). The amount of ZnTPPS incorporated in the transparent ZnTPPS/C10S/LDH film was determined from the absorption spectrum of its dimethyl sulfoxide (DMSO) solution, which was prepared by dissolving the residual ZnTPPS collected by evaporation in DMSO. The PL spectra of the transparent ZnTPPS/C10S/LDH film were measured using a spectrofluorometer (FP-6600, JASCO, Tokyo, Japan) at 25 ℃ under ambient conditions. The AFM images were acquired using a scanning probe microscope (SPM-9700, Shimadzu, Kyoto, Japan).

All chemicals used for the synthesis, purification, analysis, and antioxidant assays, with appropriate grade purity, were purchased from local suppliers and were used as supplied. The melting points were measured using an MPM-H1 melting point device (Schorpp Gerätetechnik, Überlingen, Germany), based on the glass capillary method. The MS spectra of the compounds were recorded using an Agilent 1100 series device in positive ionization mode for intermediate compound 3 and in negative ionization mode for the final compounds 5a–l, connected to an Agilent Ion Trap SL mass spectrometer (70 eV) instrument (Agilent Technologies, Santa Clara, CA, USA). The IR spectra were recorded under vacuum, using a FT/IR 6100 spectrometer (Jasco, Cremella, Italy) in KBr pellets. The ¹H-NMR and ¹³C-NMR spectra were recorded using an Avance NMR spectrometer (Bruker, Karlsruhe, Germany) in dimethyl sulfoxide-d₆. Chemical shift values were reported in δ units, relative to tetramethylsilane as internal standard.

For all synthesis, purification, structural analysis, and in vitro biological activity evaluation, the reagents were purchased from local suppliers and used in accordance with the instructions.
Melting points were measured using a melting point device MPM-H1 (Schorpp Gerätetechnik, Überlingen, Germany), based on the glass capillary method.
The IR spectra were recorded in KBr pellets, under vacuum, with a FT/IR 6100 spectrometer (Jasco, Cremella, Italy).
An Agilent Ion Trap SL mass spectrometer (70 eV) instrument (Agilent Technologies, Santa Clara, CA, USA) was used in negative ionization mode, for the final compounds 5a–d and 6a–d.
¹H-NMR and ¹³C-NMR spectra were recorded using an Avance NMR spectrometer (Bruker, Karlsruhe, Germany), in dimethylsulfoxide-d₆ (DMSO-d₆). The calibration of the spectrometer was made using tetramethylsilane. The following abbreviations for peak patterns were used to identify the multiplicity of the signals in the ¹H-NMR spectra: br-broad, s-singlet, d-doublet, dd-double doublet, t-triplet, td-triplet of doublets, q-quartet, quint-quintet, sext-sextet and m-multiplet, respectively. For the signals given by the hydrogen or carbon atoms, to describe the location of the atom in a specific region of the molecule, some abbreviations were used: Q-quinazolin-4(3H)-one, Bz-benzyl and Ar-phenolic benzene ring.

The FT-IR analysis of the treated and untreated TPCS/PW/CCF composites was conducted using FTIR machine JASCO FTIR-6100 Spectrometer (Tokyo, Japan), equipped with an ATR platinum diamond crystal and the manufacturer’s OMNIC software, to examine the functional groups’ changes in their fiber surfaces. All spectra within 4000–400 cm⁻¹ range were recorded with a resolution of 4 cm⁻¹, for a total of 32 scans per measurement. The ATR crystal was cleaned with ethanol prior to each measurement, and the background spectra were collected and removed automatically from the recorded spectrum. An anvil was used to press the sample down into the ATR diamond crystal before the test was performed in order to create close proximity between the ATR diamond crystal and the sample. The anvil pressure setting of the ATR-FTIR was subject to variation and was not reproducible throughout all samples due to the manual adjustment. OMNIC software was used to control and correct the baseline of measurements after the correct band of spectrum was selected.

Optical rotation was measured using a P-1010 polarimeter (Jasco) in chloroform at 27 °C. IR spectra were recorded on a FT/IR-6100 spectrometer (Jasco). NMR spectra were recorded on a 500 MHz NMR AVANCE III (Bruker) using deuterated chloroform (CDCl₃) and deuterated benzene (C₆D₆). MS spectra were obtained using a SYNAPT HDMS system (Waters). Preparative TLC was performed using silica gel plates (Merck Kieselgel 60 F₂₅₄). Silica gel (Kanto Chemical, Silica gel 60 N, spherical, neutral, 100–210 µm) was used for column chromatography. Semi-preparative HPLC was performed on a Shimadzu HPLC system with a Cosmosil πNAP (10 × 250 mm) column.