Viraductin

Manufactured by Cell Biolabs

Sourced in United States

ViraDuctin is a cell culture reagent designed to enhance viral transduction efficiency. It facilitates the binding and internalization of viruses into target cells, thereby improving the efficiency of viral-mediated gene delivery or infection.

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Lab products found in correlation

Adenovirus purification kit, by Cell Biolabs (2 mentions) Transduction reagents, by Cell Biolabs (2 mentions) Adenovirus titration kit, by Cell Biolabs (2 mentions) Hanks balanced salt solution (hbss), by Corning (1 mentions) Dulbecco modified eagle medium (dmem), by Corning (1 mentions) Virasafe lentiviral packaging system, by Cell Biolabs (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)

5 protocols using viraductin

Aortic SMC and Fibroblast Cultures

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Primary rat aortic SMCs were maintained in Eagle’s MEM Medium
(EMEM, Corning cellgro, Cat# 10–009-CV) containing 10% fetal calf serum
(FCS), 100 U/mL penicillin and 100 mg/mL streptomycin as described
previously^{1 (link)}. Primary
rat aortic fibroblasts (Cellbiologics, Cat# RA-6075) were cultured in
Dulbecco’s Modified Eagle’s Medium (DMEM, Corning cellgro, Cat#
10–017-CV) containing 10% fetal calf serum (FCS), 100 U/mL penicillin and
100 mg/mL streptomycin.
Adenovirus transductions were performed with ViraDuctin (Cell Biolabs,
Cat# AD-201) according to manufacturer protocol. Adenoviruses encoding PTEN
(Vector Biolabs, Cat# 1547) and GFP (Vector Biolabs, Cat# 1060) were used at 50
MOI for gene overexpression. Cell were rinsed with HBSS (Corning cellgro, Cat#
21–021-CV) 24 hours after the transduction and maintained in media with
10% FCS for 48 hours before switching to low serum media (0.1% FCS) for 48
hours. AngII or solvent (saline) were added to the cells without media change to
the final concentration of 100nM for 3 hours.
Pharmacological induction of PTEN expression was performed with
5-azacytidine (5-aza, Sigma-Aldrich, Cat# A2385). SMCs were allowed to attach in
EMEM with 10% FCS for 24 hours before switching to low serum media for 24 hours.
5-aza pre-treatment was performed in EMEM with 1% FBS at final concentration of
10μM for 24 hours before AngII/solvent treatment.

Lu S., Strand K.A., Mutryn M.F., Tucker R.M., Jolly A.J., Furgeson S.B., Moulton K.S., Nemenoff R.A, & Weiser-Evans M.C. (2019). PTEN protects against Angiotensin II-induced pathological vascular fibrosis and remodeling. Arteriosclerosis, thrombosis, and vascular biology, 40(2), 394-403.

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Lentiviral Transduction of Peritoneal Metastatic Cells

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Forty thousand 293FT cells with 10 ml of DMEM supplied with 10% of FBS and 1% of penicillin streptomycin were seeded on a 100 pi tissue culture dish. After incubating for 24 hours at 37°C and 5% CO₂, the culture medium was changed to 10 ml of Opti-MEM, and short hairpin RNA targeting CNN3 and control vector were treated using ViraSafe Lentiviral Packaging System, Pantropic (CELL BIOLABS, INC., San Diego, CA), and Lipofectamine 3000 (Invitrogen) in accordance with manufacturer’s protocol. After 48 hours, the viral soup was harvested and filtered through a 0.45-μm pored filter (Sartorius Stedim Biotech SA, Göttingen, Germany). The harvested viral soup was aliquoted into a 1.5-ml tube and kept at −70°C. Fifty thousand peritoneal metastatic cells were seeded on 24-well tissue culture plate with 0.5 ml of RPMI1460 medium and incubated at 37°C in an atmosphere of 5% CO₂ and 95% air for 24 hours. Viral transduction was performed using ViraDuctin (CELL BIOLABS, INC., San Diego, CA) according to manufacturer’s protocol.

Kim S.C., Hong C.W., Jang S.G., Kim Y.A., Yoo B.C., Shin Y.K., Jeong S.Y., Ku J.L, & Park J.G. (2018). Establishment and Characterization of Paired Primary and Peritoneal Seeding Human Colorectal Cancer Cell Lines: Identification of Genes That Mediate Metastatic Potential. Translational Oncology, 11(5), 1232-1243.

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Optimizing Adenoviral Transduction in HUVECs

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The conditions used for the transduction of recombinant adenoviruses were optimized by using adenovirus encoding GFP. All reagents and kits, including transduction reagents, an adenovirus purification kit, and an adenovirus titration kit, were purchased from Cell Biolabs, Inc. After purification, the titration of each recombinant adenovirus was determined by an ELISA titrating kit. HUVECs were seeded into 6-well plates for 24 h until they reached 80% confluence. According to the manufacturers’ protocol, adenovirus was transduced into cells by using ViraDuctin (Cell Biolabs, Inc.). HUVECs were infected with adenoviral vectors with a multiplicity of infection (MOI) of 100 plaque-forming units per cell in the presence of ViraDuctin. After incubation with viral particles for 48 h, the cells were assessed for the expression of the transduced genes.

Haidari M., Zhang W., Willerson J.T, & Dixon R.A. (2014). Disruption of endothelial adherens junctions by high glucose is mediated by protein kinase C-β–dependent vascular endothelial cadherin tyrosine phosphorylation. Cardiovascular Diabetology, 13, 112.

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Investigating miR-100-5p in BV-2 cells

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The BV-2 cells were inoculated into a 6-well plate (1×10⁵/well). When 80% of the plate was covered with cells, the medium was replaced with serum-free medium for culture for another 24 h. Following this, lentivirus vector harboring negative control sequence (miR-100-5p NC) and miR-100-5p mimic sequence (miR-100-5p OE, about 1.2 to 1.6-fold upregulated, compared with NC group) was transfected to cells. The transfected concentration of miR-100-5p was 30 pmol. In addition, lentivirus harboring an inhibitor of miR-100-5p (miR-100-5p KD) were prepared into a complex and transfected into cells (1×10⁵/mL) according to the instructions of ViraDuctin™ (Cell Biolabs, San Diego, CA, USA). The medium was then replaced, and the cells were cultured for 48 h. The cells were divided into an empty vector group and an miR-100-5p–OE group for later experiments.

Cao X., Zhang X., Chen J., Sun Q., Sun Y., Lin N, & Liu X. (2023). miR-100-5p activation of the autophagy response through inhibiting the mTOR pathway and suppression of cerebral infarction progression in mice. Aging (Albany NY), 15(16), 8315-8324.

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Optimizing Adenoviral Transduction in HUVECs

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