D90 dslr camera

The new collections of Parapterulicium are deposited at FLOR, INPA and RB. Herbarium acronyms follow Index Herbariorum (Thiers continuously updated). Macroscopic analyses were conducted following the traditional methods of Largent (1986) .
Microscopic analyses were adapted from Largent et al. (1977) for pterulaceous fungi, where, instead of sectioning the basidiomes with a razor, part of the fungus was dissected with the aid of two small diameter needles. The dissections were mounted in 5% KOH, H₂O, Melzer’s reagent, Congo red or 1% phloxine and then observed with an Olympus CX21 (Olympus, Tokyo, Japan) light microscope equipped with 10x, 40x and 100x objective lenses, the latter being used with immersion oil. Macro- and microscopic illustrations were based on pictures taken in the field with a Nikon D90 DSLR camera (Nikon, Tokyo, Japan) and on photos taken during microscopic observations. The colour codes follow the Munsell Soil Color Charts (Munsell 1975 ). Scanning electron microscopy (SEM) images were obtained using a Hitachi S-4700 field emission scanning electron microscope (Hitachi, Tokyo, Japan) and the air-dried specimens were directly stuck on the carbon tab on the stubs without any treatment. The stubs were coated with gold and platinum and examined and photographed at 5 kV.

The surface wettability of Ti-discs in each surface was also examined using the sessile drop technique, as previously described [17 (link),18 (link)]. Briefly, 1 uL of saline solution was pipetted on each disc and, immediately, a Nikon D90 DSLR camera (Nikon Corporation, Tokyo, Japan) with an 18–105 mm lens was used to photograph the samples. The water contact angle was then measured using ImageJ 1.52 q for Mac OS X (USA).

The surface wettability was investigated using the sessile drop technique, as previously described [3 (link),16 (link)]. Briefly, 1 µL of saline solution was pipetted on each disc and immediately a Nikon D90 DSLR camera (Nikon Corporation, Tokyo, Japan) with an 18–105 mm lens was used to photograph the samples. The water contact angle was then measured using ImageJ 1.52 q for Mac OS X (National Institute of Health, Bethesda, MD, USA).

The sessile drop method was used to measure the wetting properties of the groups, as previously described [23 (link)]. A Nikon D90 DSLR Camera (Nikon Corporation, Tokyo, Japan) with 18–105 mm Lens was used to photograph the samples (Fig. 6). The water contact angle and the wetted area were measured by using Image J 1.52q for Mac OS X (USA). Five samples of each group were observed.