All NPs were examined using TEM (JEOL 1200 EX II TEM) for their core size. CHO cells were incubated with Au50 or T-Au + Au 50 in DMEM medium for 1 h, then processed and imaged by with JEOL 1200 EX II TEM (JEOL) as previously described18 (link).
1200 ex 2 tem
Manufactured by JEOL
Sourced in Japan
The JEOL 1200 EX II TEM is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of materials at the nanoscale. It is equipped with an electron gun and a series of electromagnetic lenses that focus the electron beam onto the sample, allowing for the study of the internal structure and composition of materials.
Automatically generated - may contain errors
Lab products found in correlation
Orius 600, by Ametek (1 mentions)
Keenviewii, by Olympus (1 mentions)
Uranyless em stain, by Electron Microscopy Sciences (1 mentions)
Analysis five, by Olympus (1 mentions)
Tecnai g2 spirit biotwin tem, by Thermo Fisher Scientific (1 mentions)
Durcupan epoxy resin, by Merck Group (1 mentions)
Eagle 4k hs digital camera, by Thermo Fisher Scientific (1 mentions)
Gatan digital camera, by Ametek (1 mentions)
Uct ultramicrotome, by Leica (1 mentions)
Ft ir 4100 spectrometer, by Jasco (1 mentions)
Digital camera, by JEOL (1 mentions)
Nta software version 2, by Malvern Panalytical (1 mentions)
Tsg101, by Abcam (1 mentions)
Gm130, by BD (1 mentions)
Eclipse 800, by Nikon (1 mentions)
Vs120, by Olympus (1 mentions)
Fv3000, by Olympus (1 mentions)
13 protocols using 1200 ex 2 tem
1
TEM Imaging of Cellular Uptake of Au NPs
2
Ultrastructural Analysis of TBM-2 Treated Cells
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Cells, either treated with TBM-2 or not, were fixed with 2% glutaraldehyde in PBS, and samples were processed and embedded by the electron microscopy facility at Zhejiang University, Hangzhou. Images were taken with JEOL 1200 EX II TEM (JEOL, Tokyo, Japan) on the basis of the manufacturer’s instructions.
3
Ultrastructural Analysis by TEM
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TEM analysis was performed using standard techniques. The submitted tissues were retrieved from paraffin blocks, deparaffinized, post-fixed in 1% buffered osmium tetroxide, dehydrated, and embedded in Epon. Ultrathin sections (1 μm) were stained with uranyl acetate-lead citrate and examined using a JEOL 1200 EX-II TEM (Jeol, Tokyo, Japan) [18 (link)].
4
TEM Imaging of Diluted Samples
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To prepare the TEM samples, 20 μL of diluted samples in DI water was added to the TED PELLA Formvar/Carbon 200 mesh, copper grid. The sample was allowed to air dry. Images were taken using JEOL 1200 EX II TEM (Tokyo, Japan) operating at 60 kV with a Gatan Orius 600 digital camera.
5
Characterization of Synthesized Nanoparticles
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Nanoparticles from the first stage of synthesis were characterized by scattering electron microscopy (JEOL, JSM-7800F, SEM, Akishima, Tokyo, Japan) using an acceleration voltage of 2.0 kV and images were obtained with a magnification of 30,000×. Images of FCSNP were obtained by transmission electron microscopy (JEOL 1200 EX II TEM, Peabody, MA, USA) operating at 60 kV and images were obtained with 3 K and 30 K magnifications. In both cases (SEM and TEM experiments), the samples were air-dried before using them.
6
Transmission Electron Microscopy of PPC1 Cells
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After R-Au uptake, PPC1 cells were fixed with 2% glutaraldehyde in PBS
and samples were processed and embedded by the electron microscopy facility at University
of California, San Diego. Images were taken with JEOL 1200 EX II TEM (JEOL) based on
manufacturer's instruction.
and samples were processed and embedded by the electron microscopy facility at University
of California, San Diego. Images were taken with JEOL 1200 EX II TEM (JEOL) based on
manufacturer's instruction.
7
Polysome Particle Imaging and Analysis
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Polysomal fractions from CHX- and HRN-treated cells were pooled and concentrated using concentration tubes (30 kDa filter, Pall) to 50 to 100 μl concentrate. Thereby, buffer was changed several times using the polysome gradient buffer without sucrose. Samples were then collected on formvar coated copper grids (Plano) and stained with Uranyless EM Stain (Electron Microscopy Sciences) for 5 min. Grids were washed three times with double distilled water. Imaging was carried out using the JEOL-1200EX II TEM (JEOL Ltd) at 60 kV. Images were taken using a digital camera (Keen ViewII; Olympus) and processed with the iTEM software package (analySIS Five; Olympus; https://www.olympus-global.com/en/news/2005a/nr050118asfe.html ). RNA particle analysis was performed blindly. At least 54 particles (nCHX,-RNase = 54, nCHX,+RNase = 91, nHRN,-RNase = 76, nHRN,+RNase = 65) were randomly selected and measured using ImageJ (https://imagej.net/ij/ ).
8
Transmission Electron Microscopy of NSCs
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Transmission electron microscopy was performed human and mouse NSCs. Samples were immersed in modified Karnovsky's fixative (2.5% glutaraldehyde and 2% paraformaldehyde in 0.15 M sodium cacodylate buffer, pH 7.4) for at least 4 h, post‐fixed in 1% osmium tetroxide in 0.15 M cacodylate buffer for 1 h, and stained en bloc in 2% uranyl acetate for 1 h. Samples were dehydrated in ethanol, embedded in Durcupan epoxy resin (Sigma‐Aldrich), sectioned at 50–60 nm using a Leica UCT ultramicrotome, and picked up on Formvar and carbon‐coated copper grids. Sections were stained with 2% uranyl acetate for 5 min and with Sato's lead stain for 1 min. Grids were viewed with (i) a JEOL 1200EX II TEM (JEOL, Peabody, MA) and photographed using a Gatan digital camera (Gatan, Pleasanton, CA) or (ii) a Tecnai G2 Spirit BioTWIN TEM equipped with an Eagle 4k HS digital camera (FEI, Hilsboro, OR).
9
Transmission Electron Microscopy of PPC1 Cells
10
Liposome Characterization by TEM and FTIR
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Liposome suspensions were imaged, using a transmission electron microscope (TEM). Dilution of samples 10 times was done using distilled water. Equivalent volumes of both ammonium molybdate 2% solution and the diluted sample were mixed and left for 3 min at room temperature. A drop of this mixture solution was placed on a copper mesh for 5 min then by using filter papers the excess liquid was drawn off. Examination of the mesh was done using JEOL 1200-EX II TEM (JEOL Ltd, Tokyo, Japan) in the Central Lab at Ain Shams University according to Mozafari et al (2014) .
Fourier Transform Infrared Spectroscopy (FTIR) spectra of lyophilized blank, and clove oil encapsulated into liposomes were measured from 400 to 4000 cm -1 by FT-IR spectrometer 4100 (JASCO, Japan), was done in the Micro Analytical Center at Cairo University.
Fourier Transform Infrared Spectroscopy (FTIR) spectra of lyophilized blank, and clove oil encapsulated into liposomes were measured from 400 to 4000 cm -1 by FT-IR spectrometer 4100 (JASCO, Japan), was done in the Micro Analytical Center at Cairo University.
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