Embryos at late ball stage were mounted on a 5% agar pad without azide and were washed repeatedly to remove bacteria before mounting. Images were collected using a Weatherstation environmental chamber set at 20°C on a DeltaVision Image Restoration Microscope (Applied Precision) with an Inverted Olympus IX-70 microscope, and using a 63x silicone oil-immersion objective and a Photometrics CoolSnap HQ camera (Roper Scientific). Time-lapse images were acquired until the embryo began to twitch at early 1.5-fold stage. A stack of optical sections at 0.5 um spacing was acquired at 12 min intervals, using conditions for non-saturated signal, 10-32% power, and exposure times of 0.2-0.5 sec, depending on the labeling reporter used. Image acquisition did not result in appreciable developmental arrest. Between time points the focal midpoint of the stack was adjusted to compensate for rotation of the embryo and movement of the cell of interest. Deconvolution of DeltaVision Images was performed with Softworx (Applied Precision).
Ix 70 microscope
Manufactured by Teledyne
Sourced in Japan
The IX-70 is a high-performance microscope designed for advanced imaging and analysis applications. It features a modular design that allows for customization and accommodates a wide range of accessories and techniques, including brightfield, darkfield, phase contrast, and fluorescence microscopy. The IX-70 offers exceptional optical performance and is suitable for a variety of scientific and industrial research applications.
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Lab products found in correlation
4 protocols using ix 70 microscope
1
Time-lapse Imaging of Embryo Development
2
Ratiometric Imaging of Calcium Dynamics
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Ratiometric imaging was performed with a wide-field DeltaVision CORE microscope (GE-Applied Precision, Olympus Ix70 microscope, 20× 0.75NA dry lens, Roper Scientific CascadeII ECCD camera). Ca2+ measurements were performed using two fluorescent dyes- Calcium Green-1 Dextran (Ca2+ sensitive) and Texas Red Dextran (Ca2+ insensitive volume marker). Ratio image calculation was performed in Fiji (Image J) and displayed in pseudocolour. All other images were acquired with an inverted Leica SP5 Confocal microscope under a 20× 0.7NA immersion objective. Experiments requiring mechanical pressure utilised a mounted microinjection apparatus (Olympus). Myr-GCaMP5 was driven by the Gal4/UAS system. GCaMP5 Kd for Ca2+ = 660 nM (Melom and Littleton, 2013 (link)). Fiji (Image J) was used to manipulate the images for the figures.
3
Time-lapse Imaging of Embryo Development
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Embryos at late ball stage were mounted on a 5% agar pad without azide and were washed repeatedly to remove bacteria before mounting. Images were collected using a Weatherstation environmental chamber set at 20°C on a DeltaVision Image Restoration Microscope (Applied Precision) with an Inverted Olympus IX-70 microscope, and using a 63x silicone oil-immersion objective and a Photometrics CoolSnap HQ camera (Roper Scientific). Time-lapse images were acquired until the embryo began to twitch at early 1.5-fold stage. A stack of optical sections at 0.5 um spacing was acquired at 12 min intervals, using conditions for non-saturated signal, 10-32% power, and exposure times of 0.2-0.5 sec, depending on the labeling reporter used. Image acquisition did not result in appreciable developmental arrest. Between time points the focal midpoint of the stack was adjusted to compensate for rotation of the embryo and movement of the cell of interest. Deconvolution of DeltaVision Images was performed with Softworx (Applied Precision).
4
Fluorescence Spectroscopy and Microscopy
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Steady-state fluorescence spectra were recorded using a Fluorolog 3 (Jobin-Yvon) steady-state spectrometer. Fluorescence microscopy experiments were carried out on an Olympus IX70 microscope (Japan), equipped with a Roper Scientific Inc. MicroMAX camera with an UPlanFL100×/1.4 objective. Images were processed with "WINView" software.
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