Palbociclib (S1116), abemaciclib (S7158), and ribociclib (S7440) were purchased from Selleck Chemicals. CR-1–31-B and silvestrol were synthesized as described previously (26 (link), 27 (link)). Antibodies against HSP90 (H-114), Cyclin D1 (M20), Cyclin D3 (DCS28), Cyclin A2 (BF683), Cyclin E1 (HE12), Cyclin E2 (A-9), CDK2 (D-12), CDK4 (DCS-35), CDK6 (C-21), p16 (C-20), p21 (H164), and p27 (C-19) were purchased from Santa Cruz Biotechnology; p-RB (S795) and Cyclin D2 (D52F9) were purchased from Cell Signaling Technology; RB (554136) was purchased from BD Pharmingen; and eIF4A1 (ab31217) was purchased from Abcam.
Rb 554136
Manufactured by BD
The RB (554136) is a lab equipment product designed for use in scientific and medical research environments. It serves as a core function to perform necessary tasks related to the intended application. The product specifications and technical details are available upon request.
Automatically generated - may contain errors
Lab products found in correlation
Ribociclib, by Selleck Chemicals (1 mentions)
Abemaciclib, by Selleck Chemicals (1 mentions)
P rb s795, by Cell Signaling Technology (1 mentions)
Palbociclib, by Selleck Chemicals (1 mentions)
Ab49900, by Abcam (1 mentions)
Pcw57, by Addgene (1 mentions)
Pentr2b, by Addgene (1 mentions)
β actin, by Abcam (1 mentions)
2 protocols using rb 554136
1
Cell Cycle Regulators Profiling
2
Western Blot Analysis of Cell Cycle Regulators
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The following antibodies were used for western blot analysis: beta-Actin, ab49900 (Abcam); CDK6, D4S8S (Cell Signalling); Clathrin (C28, BD Biosciences); Cyclin D2, 10934-1-AP (Proteintech); Phospho-Rb (Ser780), 9307 (Cell Signalling); Phospho-Rb (Thr821), ab4787(Abcam); Rb, 554136 (BD Pharmingen); RUNX1/ETO, PC285 (Merck Millipore).
Growth factors and media were obtained from StemCell Technologies, Sigma- Aldrich, Gibco and R&D Systems. Palbociclib was purchased from DC Chemicals (Shanghai, China).
dnFOS was amplifided from cDNA provided by Charles Vinson (Olive et al., 1997 (link)) with SalI and NotI restriction site overhangs. Using these restriction sites, the fragment was ligated into pENTR2B (Addgene) and then recombined into pCW57.1 (Addgene).
Growth factors and media were obtained from StemCell Technologies, Sigma- Aldrich, Gibco and R&D Systems. Palbociclib was purchased from DC Chemicals (Shanghai, China).
dnFOS was amplifided from cDNA provided by Charles Vinson (Olive et al., 1997 (link)) with SalI and NotI restriction site overhangs. Using these restriction sites, the fragment was ligated into pENTR2B (Addgene) and then recombined into pCW57.1 (Addgene).
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