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Fluoview fv1000 filter confocal microscope system

Manufactured by Olympus
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The FluoView FV1000-Filter Confocal Microscope System is a high-performance confocal laser scanning microscope. It is designed for high-resolution imaging and analysis of fluorescent samples.

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Lab products found in correlation

Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (3 mentions) Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (3 mentions) Alexa fluor 488 phalloidin, by Olympus (2 mentions) Fluoview viewer software ver 4, by Olympus (2 mentions) Alexa 488 phalloidin, by Thermo Fisher Scientific (2 mentions) Tissue tek oct compound, by Sakura Finetek (1 mentions) Lab tekii 8 well chamber slide, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

FluoView FV1000 confocal microscope system FluoView FV1000 confocal microscope Fluoview FV1000 confocal system FV1000 Filter confocal microscope Fluoview system (Fv1000 Olympus Microscope) FluoView FV1000 confocal Fluoview FV1000 microscope Fluoview FV1000 system FV1000 confocal microscope FluoView confocal microscope

6 protocols using fluoview fv1000 filter confocal microscope system

1

Visualizing Glioblastoma Cell Internalization of FA-3WJ-LNA-miR21-Alexa647

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Example 4

This study shows the distribution of FA-3WJ-LNA-miR21-Alexa647 RNP in GBM30 cells after 2 hrs of incubation was visualized by confocal fluorescent microscopy (FIG. 11). The image shows successful internalization of FA-3WJ-LNA-miR21-Alexa647 RNP into GBM30 cells and accumulation in cytoplasm not much in nucleus. Since LNA-miR21 will work against mature miR-21 in cytoplasm to show its small RNA interfering activity, the cytoplasmic distribution of FA-3WJ-LNA-miR21-Alexa647 RNP promises the drugability in target therapy of glioblastoma. Alexa647 was expressed in red peudocolor. The cytoskeleton of the fixed cells was stained by Alexa Fluor 488 Phalloidin (Invitrogen, Grand Island, N.Y.) and the nucleus stained with 0.01% DAPI solution. Fluorescence microscopy was performed using Olympus 4-filter-based FluoView FV1000-Filter Confocal Microscope System (Olympus Corp.) at the wavelengths of 461 nm (for the cell nucleus stained by DAPI), 530 nm (for the cytoskeleton stained by Alexa Fluor 488 Phalloidin) and 665 nm (for the Alexa647). Images were analyzed by Olympus FluoView Viewer software ver. 4.0 (Olympus). The fluorescent images were obtained using FluoView FV1000-Filter Confocal Microscope System (Olympus Corp.).

US11325939B2. RNA nanoparticles for brain tumor treatment (2022-05-10). University of Kentucky Research Foundation [US]. Inventors: Peixuan Guo [US], Carlo M. Croce [US], Tae Jin Lee [US], Farzin Haque [US], Hui Li [US].
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2

Visualization of FA-3WJ-LNA-miR21-Alexa647 RNP Internalization in GBM30 Cells

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Example 4

This study shows the distribution of FA-3WJ-LNA-miR21-Alexa647 RNP in GBM30 cells after 2 hrs of incubation was visualized by confocal fluorescent microscopy (FIG. 11). The image shows successful internalization of FA-3WJ-LNA-miR21-Alexa647 RNP into GBM30 cells and accumulation in cytoplasm not much in nucleus. Since LNA-miR21 will work against mature miR-21 in cytoplasm to show its small RNA interfering activity, the cytoplasmic distribution of FA-3WJ-LNA-miR21-Alexa647 RNP promises the drugability in target therapy of glioblastoma. Alexa647 was expressed in red peudocolor. The cytoskeleton of the fixed cells was stained by Alexa Fluor 488 Phalloidin (Invitrogen, Grand Island, N.Y.) and the nucleus stained with 0.01% DAPI solution. Fluorescence microscopy was performed using Olympus 4-filter-based FluoView FV1000-Filter Confocal Microscope System (Olympus Corp.) at the wavelengths of 461 nm (for the cell nucleus stained by DAPI), 530 nm (for the cytoskeleton stained by Alexa Fluor 488 Phalloidin) and 665 nm (for the Alexa647). Images were analyzed by Olympus FluoView Viewer software ver. 4.0 (Olympus). The fluorescent images were obtained using FluoView FV1000-Filter Confocal Microscope System (Olympus Corp.).

US10584144B2. RNA nanoparticles for brain tumor treatment (2020-03-10). UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION [US]. Inventors: Peixuan Guo [US], Carlo M. Croce [US], Tae Jin Lee [US], Farzin Haque [US], Hui Li [US].
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3

Cellular Uptake of pRNA-3WJ-PTX Micelles

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KB cells were grown on glass slides overnight. 1 μM Alexa647 labeled pRNA-3WJ-PTX micelles and the control pRNA-3WJ-PTX nanoparticles without lipophilic module were each incubated with the cells at 37°C for 1 hr. After washing with PBS, the cells were fixed by 4% paraformaldehyde (PFA) and washed 3 times by PBS. The cytoskeleton of the fixed cells was treated with 0.1% Triton-X100 in PBS for 5 min to improve cell membrane permeability and then stained by Alexa Fluor 488 Phalloidin (Life Technologies) for 30 min at room temperature and then rinsed with PBS for 3 × 10 min. The cells were mounted with Prolong® Gold antifade reagent with DAPI (Life Technologies) and DAPI was used for staining the nucleus. The cells were then assayed for binding and cell entry by FluoView FV1000-Filter Confocal Microscope System (Olympus Corp.).
Shu Y., Yin H., Rajabi M., Li H., Vieweger M., Guo S., Shu D, & Guo P. (2018). RNA-based micelles: A novel platform for paclitaxel loading and delivery. Journal of controlled release : official journal of the Controlled Release Society, 276, 17-29.
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4

Fluorescence Imaging of pRNA-3WJ Nanoparticles

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To investigate the delivery of pRNA-3WJ nanoparticles in vivo, a fluorescence imaging study was performed after tail vein injection of 100 μL 20 uM Alexa647 labeled 3WJ-EGFRapt/anti-miR-21 into orthotopic TNBC tumor mice. PBS injected mice were used as fluorescence negative control, The mice were sacrificed at 8 h post injection by inhalation of CO2 followed by cervical dislocation, and major internal organs including heart, lungs, liver, spleen, kidneys together with tumor from the sacrificed mice were collected and subjected to fluorescence imaging for assessment of biodistribution profile using IVIS Spectrum station (Caliper Life Sciences) with excitation at 640 nm and emission at 680 nm. The tumors were further fixed in 4% PFA with 10% sucrose in PBS overnight at 4 °C and embedded in Tissue-Tek O.C.T. compound (Sakura Finetek USA, Inc.) for frozen sectioning (10 μm thick). The sectioned samples were mounted by ProLong Gold Antifade Reagent with DAPI (Life Technologies) overnight. The fluorescent images were obtained using FluoView FV1000-Filter Confocal Microscope System (Olympus) (available at the University of Kentucky Markey Cancer Center Imaging core facility).
Shu D., Li H., Shu Y., Xiong G., Carson WE I.I.I., Haque F., Xu R, & Guo P. (2015). Systemic Delivery of Anti-miRNA for Suppression of Triple Negative Breast Cancer Utilizing RNA Nanotechnology. ACS Nano, 9(10), 9731-9740.
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5

Cellular Uptake and Localization of Multimodal Nanoparticles

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MDA-MB-231 cells were grown on Lab-TekII 8-well chamber slide (Nunc) in DME/F-12 (1:1) medium overnight. 100 nM concentration of Alexa647 labeled 3WJ-EGFRapt/anti-miR-21 and the control 3WJ, 3WJ-EGFRapt, and 3WJ-anti-miR-21 were each incubated with the cells at 37 °C for 2 h. After washing with PBS, the cells were fixed by 4% paraformaldehyde (PFA) and washed 3 times by PBS. The cytoskeleton of the fixed cells was stained by Alexa488 Phalloidin (Life Technologies) for 30 min at room temperature and then rinsed with PBS for 3 × 10 min. The cells were mounted with Prolong Gold antifade reagent. DAPI (Life Technologies) was used for staining the nucleus. The cells were then assayed for nanoparticles binding and cellular entry by FluoView FV1000-Filter Confocal Microscope System (Olympus) (available at the University of Kentucky Markey Cancer Center imaging core facility).
Shu D., Li H., Shu Y., Xiong G., Carson WE I.I.I., Haque F., Xu R, & Guo P. (2015). Systemic Delivery of Anti-miRNA for Suppression of Triple Negative Breast Cancer Utilizing RNA Nanotechnology. ACS Nano, 9(10), 9731-9740.
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6

Cellular Uptake of RNA Nanotubes

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Prostate cancer LnCap cells were grown on glass cover slides in 24-well plates in RPMI-1640 medium with 10% FBS at 37 °C in humidified air containing 5% CO2 overnight. 2’F-modified RNA nanotubes harboring cholesterol and labeled by Cy5 were diluted in optium-MEM medium to 100 nM and incubated with the cells for 2 h at 37 °C. RNA nanotubes without cholesterol and single strand RNA were used as the negative control. After the incubation, the cells were washed with PBS and fixed by 4% paraformaldehyde. Alexa 488 Phalloidin (Life Technologies) was used to stain the cellular actin. The Prolong Gold antifade reagent with DAPI (Life Technologies) were then used to stain the cell nucleuses and mount the cells to the glass slides. The confocal images were recorded by the FluoView FV1000-Filter Confocal Microscope System (Olympus).
Li H., Wang S., Ji Z., Xu C., Shlyakhtenko L.S, & Guo P. (2019). Construction of RNA nanotubes. Nano research, 12(8), 1952-1958.
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