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2 protocols using vac isq

1

Modulation of T-cell Activation and Cytokine Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
IL-1Ra (R&D Systems; 480-RM, 50ng/mL), rTNFα (Peprotech; 315–01Am, 20ng/mL), αCD3e (Biolegend; 100331 for in vitro assays and 100340 for in vivo assays), αTNF (Biolegend; 506332, 20μg/mL), αFasL (Biolegend; 106608, 10μg/mL), αCD40L (Biolegend; 310812, 10 μg/mL), IL-1β ELISA (R&D Systems; DY-401), TNFα ELISA(Biolegend; 510802 for capture and 506312 for detection), Naïve T cell Mojosort (Biolegend; 480040), total CD4+ T cell Mojosort (Biolegend; 480006), Zombie yellow (Biolegend; 423103), DAPI (Biolegend; 422801), IETD (R&D Systems; FMK007, 10μM), rGMCSF (Biolegend; 576306), Anti-biotin beads (Miltenyi Biotec; 130–090-485), OVA323–339 (Invivogen; vac-isq), LPS (Sigma, 100ng/mL), ATP (Invivogen; tlrl-ATPl, 5nM), IL17a ELISA (Biolegend; 506902 for capture and 507002 for detection), EAE immunization kit (Hooke Laboratories, EK-2110) mouse MOG35–55 (CSbiologicals, CS0681)
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2

Modulation of T-cell Activation and Cytokine Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
IL-1Ra (R&D Systems; 480-RM, 50ng/mL), rTNFα (Peprotech; 315–01Am, 20ng/mL), αCD3e (Biolegend; 100331 for in vitro assays and 100340 for in vivo assays), αTNF (Biolegend; 506332, 20μg/mL), αFasL (Biolegend; 106608, 10μg/mL), αCD40L (Biolegend; 310812, 10 μg/mL), IL-1β ELISA (R&D Systems; DY-401), TNFα ELISA(Biolegend; 510802 for capture and 506312 for detection), Naïve T cell Mojosort (Biolegend; 480040), total CD4+ T cell Mojosort (Biolegend; 480006), Zombie yellow (Biolegend; 423103), DAPI (Biolegend; 422801), IETD (R&D Systems; FMK007, 10μM), rGMCSF (Biolegend; 576306), Anti-biotin beads (Miltenyi Biotec; 130–090-485), OVA323–339 (Invivogen; vac-isq), LPS (Sigma, 100ng/mL), ATP (Invivogen; tlrl-ATPl, 5nM), IL17a ELISA (Biolegend; 506902 for capture and 507002 for detection), EAE immunization kit (Hooke Laboratories, EK-2110) mouse MOG35–55 (CSbiologicals, CS0681)
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