3 4 5 dimethylthiazol 2 yl 5 3 carboxymethoxyphenyl 2 4 sulfophenyl 2h tetrazolium inner salt mts

Manufactured by Promega

Sourced in United States

3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) is a tetrazolium compound used in cell viability assays. It is a yellow compound that can be reduced by metabolically active cells to form a colored formazan product, which can be quantified spectrophotometrically.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Bovine serum albumin (bsa), by Merck Group (3 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Phenazine methosulfate, by Merck Group (2 mentions) Lactate assay kit, by Merck Group (2 mentions) Hdac activity colorimetric assay kit, by Abcam (2 mentions) Sulforhodamine b, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Trichloroacetic acid, by Merck Group (2 mentions) Cycloheximide (chx), by Merck Group (2 mentions) Muse annexin 5 dead cell kit, by Merck Group (2 mentions) Annexin 5 binding buffer, by BioLegend (1 mentions) Annexin 5, by BioLegend (1 mentions) Horseradish peroxidase hrp conjugated goat anti mouse igg h l antibody, by Abcam (1 mentions) Anti his monoclonal antibody, by Abcam (1 mentions) Propidium iodide, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) 5 5 6 6 tetrachloro 1 1 3 3 tetraethylbenzimidazolylcarbocyanine iodide jc 1, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) (43 citations) 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (19 citations) (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (7 citations) 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay kit (3 citations) 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) solution (2 citations)

16 protocols using 3 4 5 dimethylthiazol 2 yl 5 3 carboxymethoxyphenyl 2 4 sulfophenyl 2h tetrazolium inner salt mts

Recombinant Toxin Purification and Characterization

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Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (H + L) antibody and anti-His monoclonal antibody were purchased from Abcam (Cambridge, MA, USA). 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) was purchased from Promega Corporation (Madison, WI, USA). Anti-glutathione S-transferase (GST) monoclonal antibodies were purchased from EARTHOX Life Sciences (Millbrae, CA, USA). Annexin V, annexin-V-binding buffer, and PE anti-human CD235a (glycophorin A) antibodies were purchased from BioLegend (San Diego, CA, USA). DAPI (4′,6-diamidino-2-phenylindole), fluo-4, and propidium iodide were purchased from Sigma (St. Louis, MO, USA). Zaragozic acid A trisodium salt (ZA) was purchased from ChemCruz (Huissen, The Netherlands).
Recombinant toxins, 6×His-tagged ETX, GST-tagged ETX, mScar-ETX (mScarlet fluorescent protein fusion protein), CPB, Hla, and Net B proteins, were expressed and purified as previously described [32 (link),54 (link)].

Huang J., Zhao B., Liu T., Kang L., Li J., Guo Z., Chen M., Gao S., Wang J., Li Y., Wang J, & Xin W. (2023). Statins as Potential Preventative Treatment of ETX and Multiple Pore-Forming Toxin-Induced Diseases. International Journal of Molecular Sciences, 24(6), 5414.

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miRNA-141 Modulation in Cancer Cells

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RPMI-1640 medium, FBS and PBS were obtained from Gibco-BRL (Life Technologies, Paisley, Scotland). 5-FU (Sigma Chemical Co., Poole, U.K.) was diluted to 0, 12.5, and 25.0 µg/ml with RPMI-1640 medium. The nucleotide sequences (mimics, mimics control, inhibitor and inhibitor control) of miRNA-141 were designed and synthesized by RIBOBIO (Shanghai, China). 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) were obtained from Promega Corporation(Madison, WI, U.S.A.).

Wang F., Zhao L., Zhang J., Meng Z., Zhou C., Wang G., Liu Y., Li M., Xi J., Niu W, & Wang G. (2018). Chemotherapy-induced miR-141/MAP4K4 signaling suppresses progression of colorectal cancer. Bioscience Reports, 38(6), BSR20180978.

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Quercetin and Mitochondrial Apoptosis Assay

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Quercetin for the synthesis of 4Ac-Q (purity ≥ 90%) was purchased from Sigma-Aldrich (St. Louis, MO, USA), and quercetin for the bioactivity assay (purity > 99%) was purchased from LKT Laboratories (St. Paul, MN, USA). 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) was purchased from Promega (Madison, WS, USA). An annexin V-FITC apoptosis detection kit was purchased from BD Biosciences (San Diego, CA, USA). 3,8-phenanthridine diamine-hexyl triphenylphosphonium iodide (MitoSOX red) and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetra-ethyl benzimidazolyl carbocyanine iodide (JC-1) were purchased from Invitrogen-Life Technologies (Carlsbad, CA, USA). The antibodies against PARP (#9542) and anti-rabbit (#7074) secondary antibodies were from Cell Signaling Technology (Beverly, MA, USA). The human apoptosis antibody array was purchased from Abcam (ab134001; Cambridge, MA, USA). Mn (III)-tetrakis (4-benzoic acid)porphyrin chloride (MnTBAP) was purchased from Calbiochem (San Diego, CA, USA).

Sakao K., Hamamoto S., Urakawa D., He Z, & Hou D.X. (2024). Anticancer Activity and Molecular Mechanisms of Acetylated and Methylated Quercetin in Human Breast Cancer Cells. Molecules, 29(10), 2408.

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Immune Cell Signaling Assay Reagents

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Compound 48/80, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), phorbol 12-myristate 13-acetate (PMA), and calcium ionophore A23187 (A23187) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) was purchased from Promega Corporation (Madison, WI, USA). Avidin peroxidase (AP), fetal bovine serum (FBS), RPMI 1640, and Iscove's Modified Dulbecco's Medium (IMDM) were purchased from Gibco BRL (Grand Island, NY, USA). Anti-human TNF-α/IL-6/IL-8, anti-mouse immunoglobulin E (IgE)/IL-6, recombinant TNF-α/IL-6/IL-8, and biotinylated TNF-α/IL-6/IL-8 were purchased from BD Pharmingen (San Diego, CA, USA). Anti-phospho-p38, -ERK, -JNK, -Akt, -IκBα, anti-GAPDH, IκBα, and NF-κB antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Anti-p38, JNK, and histone antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Anti-Akt and ERK antibodies were purchased from Bioworld Technology (Minneapolis, MN, USA).

Kee J.Y., Jeon Y.D., Kim D.S., Han Y.H., Park J., Youn D.H., Kim S.J., Ahn K.S., Um J.Y, & Hong S.H. (2016). Korean Red Ginseng improves atopic dermatitis-like skin lesions by suppressing expression of proinflammatory cytokines and chemokines in vivo and in vitro. Journal of Ginseng Research, 41(2), 134-143.

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Resveratrol Modulates TGF-beta1 Signaling

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Resveratrol was purchased from Shanghai Standardization for the Traditional Research Center and dissolved in dimethyl sulfoxide (DMSO). Human TGF-beta1 was obtained from PeproTech (USA). 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) was purchased from Promega (Madison, WI, USA). QuicBlock^TM Blocking Buffer for Immunol Staining, Antifade Mounting Medium with DAPI, QuicBlock^TM Secondary Antibody Dilution Buffer for Immunofluorescence and QuicBlock^TM Primary Antibody Dilution Buffer for Immunol Staining were purchased from Beyotime (Shanghai, China). Antibodies against MMP-2, MMP-9, α-SMA, Smad2, Smad3, P-Smad2, P-Smad3, Snail1, and Slug were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Fibronectin, P-PI3K, PI3K, P-AKT, AKT, E-cadherin, and vimentin antibodies were purchased from Cell Signaling Technology (Boston, MA, USA). LY290042, SB431542, SP600125, PDTC, and PD98059 were purchased from Selleck Chemical (Houston, TX, USA). PDTC and PD98059 were purchased from MedChemExpresss (New Jersey, NJ, USA). IRDye^TM fluorescence antibodies were obtained from Li-Cor Bioscience (Lincoln, NE, USA).

Sun Y., Zhou Q.M., Lu Y.Y., Zhang H., Chen Q.L., Zhao M, & Su S.B. (2019). Resveratrol Inhibits the Migration and Metastasis of MDA-MB-231 Human Breast Cancer by Reversing TGF-β1-Induced Epithelial-Mesenchymal Transition. Molecules, 24(6), 1131.

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DMEM-Based Cell Viability Assay

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Dulbecco’s Modified Eagle Medium (DMEM) containing phenol red, penicillin–streptomycin, EDTA-trypsin, and Dulbecco’s phosphate buffer solution (DPBS) was purchased from Gibco BRL Life Technologies (Invitrogen™, Paisley, UK). Fetal bovine serum (FBS) was purchased from HyClone, USA. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) was purchased from Promega, USA. Hydrogen peroxide, 2′,7′dihydrofluorescein diacetate (DCFH-DA), chlorpromazine hydrochloride (CPZ), QA, neochlorogenic acid (CAS No. 906-33-2) with IUPAC name 3-O-caffeoylquinic acid (3-CQA), and cryptochlorogenic acid (CAS No. 905-99-2) with IUPAC name 4-O-caffeoylquinic acid (4-CQA) were purchased from Sigma-Aldrich, United Kingdom. Acetonitrile (LC–MS reagent) was purchased from RCI Labscan (Bangkok, Thailand). Water type I was purified from MilliQ^® Integral 3 system (Millipore, Bedford, MA, USA). Formic acid (analytical grade) was purchased from Merck (Darmstadt, Germany). A 0.45 mm nylon disposable membrane filter and syringe filter were supplied from Vertical Chromatography, Thailand. Other unmarked reagents were of analytical grade.

Chansriniyom C., Nooin R., Nuengchamnong N., Wongwanakul R., Petpiroon N., Srinuanchai W., Chantarasuwan B., Pitchakarn P., Temviriyanukul P, & Nuchuchua O. (2021). Tandem mass spectrometry of aqueous extract from Ficus dubia sap and its cell-based assessments for use as a skin antioxidant. Scientific Reports, 11, 16899.

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Comprehensive Cellular Metabolic Assays

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2-deoxy-D-glucose (2-DG), sodium butyrate (NaBt), sodium valproate (NaVPA), bovine serum albumin (BSA), phenazine methosulfate (PMS), sulforhodamine B (SRB), trichloro acetic acid (TCA), acetic acid, dimethyl sulfoxide (DMSO), cycloheximide (CHX), chloroquine (CQ), dimethyloxalylglycine (DMOG), rhodamine (Rho), 5-bromodeoxyuridine (BrdU) Cell Proliferation Assay Kit, and Lactate Assay Kit were purchased from Sigma-Aldrich, Saint Louis, MO, USA. 3,6-di-O-acetyl-2-deoxy-D-glucose (WP1122) was produced at the MD Anderson Cancer Institute by Professor Waldemar Priebe’s team and was kindly provided for research by the Dermin company, who, based on an exclusive license, conduct developmental studies of the molecule.
3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H tetrazolium, inner salt (MTS), was purchased from Promega (Madison, WI, USA). Muse™ Annexin V & Dead Cell Kit was purchased from Calbiochem, Merck Millipore (Darmstadt, Germany). HDAC Activity Colorimetric Assay Kit was purchased from BioVision (Milpitas, CA, USA). All the other reagents and solvents used in this study were of the highest analytical reagent grade.

Pająk B., Siwiak-Niedbalska E., Jaśkiewicz A., Sołtyka M., Zieliński R., Domoradzki T., Fokt I., Skóra S, & Priebe W. (2021). Synergistic Anticancer Effect of Glycolysis and Histone Deacetylases Inhibitors in a Glioblastoma Model. Biomedicines, 9(12), 1749.

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Bovine Liver Catalase Alginate Protocol

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Except for the materials listed below all materials and reagents were purchased from Sigma (St. Louis, MO, USA). All catalase used for the following experiments was derived from bovine liver, (Catalogue # C9322, Sigma, St. Louis, MO, USA). Collagenase P was purchased from Roche (Indianapolis, IN, USA). Density gradient solutions were prepared using Optiprep and University of Wisconsin solution (UWS; Perfadex, Xvivo Perfusion, Englewood, CO). High-mannuronic acid-low viscosity (LVM) alginate and high-guluronic acid-low viscosity (LVG) alginate were purchased from Nova-Matrix (Sandvika, Norway) and were reported by the manufacturer to have molecular weights 75–200 kDa and G/M ratios of ≤1 and ≥1.5, respectively. [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) was purchased from Promega (Madison, WI, USA). ¹²⁵I-insulin was purchased from Perkin Elmer (Waltham, MA).

McQuilling J.P., Sittadjody S., Pendergraft S., Farney A.C, & Opara E.C. (2017). Applications of Particulate Oxygen-Generating Substances (POGS) in the Bioartificial Pancreas. Biomaterials science, 5(12), 2437-2447.

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Evaluating Nanoparticle-Mediated Drug Delivery in Breast Cancer Cells

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DDAB, squalene, Tween 80, and PEI with an average molecular weight of 25,000 (PEI-25k) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Culture medium (RPMI-1640) and FBS were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Dulbecco’s PBS was purchased from Thermo Fisher Scientific. Ultrapure water was obtained from EMD Millipore RIOs™. Annexin V Apoptosis Detection kit was purchased from BioLegend (Shanghai, China). MCF-7 (human breast adenocarcinoma cell line) was obtained from Pasteur Institute (Tehran, Iran). 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H tetrazolium, inner salt (MTS) was purchased from Promega Corporation (Fitchburg, WI, USA). Reverse transcription-PCR (RT-PCR) Master Mix Reagents kit and cDNA Synthesis kit were purchased from Fermentas. Other chemicals were of reagent grade and used without further purification.

Mokhtary P., Javan B., Sharbatkhari M., Soltani A, & Erfani-Moghadam V. (2018). Cationic vesicles for efficient shRNA transfection in the MCF-7 breast cancer cell line. International Journal of Nanomedicine, 13, 7107-7121.

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Cytotoxicity Assay Protocol

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Phenylmethanesulfonyl (PMSF), antiprotease cocktail, Triton X-100, Hoechst 33258 and bovine serum albumin (BSA) were purchased from Sigma (St. Louis, MO). Dulbecco's modified Eagle's medium (DMEM), penicillin/streptomycin, fetal bovine serum (FBS), EDTA and L-glutamine were purchased from Gibco (Grand Island, NY). [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] was purchased from Promega (Madison, WI).

Dever D.P., Adham Z.O., Thompson B., Genestine M., Cherry J., Olschowka J.A., DiCicco-Bloom E, & Opanashuk L.A. (2015). Aryl Hydrocarbon Receptor (AhR) Deletion in Cerebellar Granule Neuron Precursors Impairs Neurogenesis. Developmental neurobiology, 76(5), 533-550.

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