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C1498

Manufactured by American Type Culture Collection
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The C1498 is a laboratory equipment product offered by the American Type Culture Collection (ATCC). It is a type of equipment used in scientific research and testing. The core function of the C1498 is to provide a controlled environment for the cultivation and maintenance of cell cultures. The detailed specifications and intended uses of the C1498 are not available in this response.

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Lab products found in correlation

Thp 1, by American Type Culture Collection (10 mentions) Mv4 11, by American Type Culture Collection (9 mentions) Penicillin, by Thermo Fisher Scientific (7 mentions) Streptomycin, by Thermo Fisher Scientific (7 mentions) Kasumi 1, by American Type Culture Collection (5 mentions) Molm 13, by American Type Culture Collection (3 mentions) Rpmi 1640, by American Type Culture Collection (3 mentions) Rpmi 1640, by Thermo Fisher Scientific (3 mentions) E g7 ova, by American Type Culture Collection (2 mentions) Raw264, by American Type Culture Collection (2 mentions) Penicillin and streptomycin, by Thermo Fisher Scientific (2 mentions) Hl 60, by American Type Culture Collection (2 mentions) Streptomycin, by STEMCELL (2 mentions) Penicillin, by STEMCELL (2 mentions) Iscove s modified dulbecco s media, by Thermo Fisher Scientific (2 mentions) Stemspan serum free expansion medium 2, by STEMCELL (2 mentions) Streptomycin, by American Type Culture Collection (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Mycoplasma contamination kit, by R&D Systems (1 mentions) Hek293f, by Thermo Fisher Scientific (1 mentions)

19 protocols using c1498

1

AML Cell Line Characterization Protocol

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Human AML cell line KG-1 (CVCL_0374), Kasumi-1 (CVCL_0589), HL-60 (CVCL_0002), U-937 (CVCL_0007), MV4-11 (CVCL_0064), AML-193 (CVCL_1071), THP-1 (CVCL_0006), mouse AML cell line C1498 (CVCL_3494), and human normal fibroblast cell line HFF-1 (CVCL_3285) were purchased from ATCC during July 2017 to February 2019. The culture condition was listed in Supplementary Materials and Methods. All human cell lines have been authenticated using short tandem repeat analysis by Pangenia lifesciences Ltd. and mouse AML cell line C1498 was authenticated using short tandem repeat analysis by ATCC during January to May 2021. Cell lines were tested mycoplasma free and the latest date for testing was April to May 2021. Cells used for experiments were kept in continuous culture for no more than 15 passages.
Cai Y., Chow J.P.H., Leung Y.O., Lu X., Yuen C.H., Lee W.L., Chau K.C., Yang L.L., Wong R.M.H., Lam J.Y.T., Chow D.T.L., Chung S.H.K., Kwok S.Y, & Leung Y.C. (2021). NEI-01-Induced Arginine Deprivation Has Potent Activity Against Acute Myeloid Leukemia Cells Both In Vitro and In Vivo. Molecular cancer therapeutics, 20(11).
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2

Cell Line Maintenance and AML Sample Analysis

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HEK293F and CHO cell lines were obtained from Life Technologies (Carlsbad). Human monocytic AML cell lines (THP-1, MV4–11 and U937), mouse leukemia cell line C1498, and mouse macrophage cell line RAW264.7 were obtained from ATCC and maintained in a humidified atmosphere of 5% CO2 at 37°C, in media suggested by ATCC supplemented with fetal bovine serum (FBS) (HyClone) and 100 U/mL penicillin and 100 μg/mL streptomycin (Life Technologies). Cell lines were not authenticated in the past year and cultured for fewer than 10 passages in indicated medium. All cell lines were routinely tested using a mycoplasma-contamination kit (R&D Systems). Primary human AML samples were obtained from the University of Texas Southwestern Medical Center (UTSW). Informed consent was obtained under a protocol reviewed and approved by the Institutional Review Board at UTSW. LILRB4 expressed samples were analyzed by flow cytometry.
Gui X., Deng M., Song H., Chen Y., Xie J., Li Z., He L., Huang F., Xu Y., Anami Y., Yu H., Yu C., Li L., Yuan Z., Xu X., Wang Q., Chai Y., Huang T., Shi Y., Tsuchikama K., Liao X.C., Xia N., Gao G.F., Zhang N., Zhang C.C, & An Z. (2019). Disrupting LILRB4/APOE interaction by an efficacious humanized antibody reverses T-cell suppression and blocks AML development. Cancer immunology research, 7(8), 1244-1257.
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3

Murine Colorectal and Myeloid Leukemia Cells

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Murine colorectal carcinoma cells (CT26.WT) were purchased from ATCC (#CRL-2638). CT26.WT cells were cultured in RPMI-1640 medium supplemented with 10% FBS and 1% penicillin/streptomycin. Prior to cell harvest, cells were seeded in T75 culture flasks and allowed to reach confluency. Once confluent, cells were harvested with Trypsin–EDTA (0.25%). Murine acute myeloid leukemia cells (C1498) were originally purchased from ATCC (#TIB-49). C1498 cells used in this study were provided by Dr. Tan (UVA). C1498 cells were cultured in RPMI-1640 medium supplemented with 10% FBS and 1% penicillin/streptomycin. Cells were monitored daily and harvested when the average cell density reached 1.0 × 106 cells/mL.
Boykov I.N., Montgomery M.M., Hagen J.T., Aruleba R.T., McLaughlin K.L., Coalson H.S., Nelson M.A., Pereyra A.S., Ellis J.M., Zeczycki T.N., Vohra N.A., Tan S.F., Cabot M.C, & Fisher-Wellman K.H. (2023). Pan-tissue mitochondrial phenotyping reveals lower OXPHOS expression and function across cancer types. Scientific Reports, 13, 16742.
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4

LILRB3 Signaling in Leukemia Cells

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THP-1, MV4–11, Molm13, U937, C1498 and 293T cells were purchased from ATCC. AML cell lines were cultured in RPMI-1640 with 10% FBS. Human anti-LILRB3 with the N297A mutation was coated onto the plate to activate LILRB3 signaling, and plates coated with human IgG (N297A) were used as controls. Cells infected with virus were cultured for at least an additional 3 weeks before analysis of LILRB3 signaling. Dead cells were identified using PI staining. Primary human T cells were isolated by autoMACS from donor PBMCs, stimulated with anti-CD3 and anti-CD28, and cultured in RPMI-1640 in the presence of IL-2. For the cytotoxic T lymphocyte assay, human AML cells were stained with CFSE and mixed at different ratios with activated T cells. After 10 hours, the percentage of PI-positive CFSE-stained AML cells was determined by FACS.
Wu G., Xu Y., Schultz R.D., Chen H., Xie J., Deng M., Liu X., Gui X., John S., Lu Z., Arase H., Zhang N., An Z, & Zhang C.C. (2021). LILRB3 supports acute myeloid leukemia development and regulates T-cell antitumor immune responses through the TRAF2–cFLIP–NF-κB signaling axis. Nature cancer, 2(11), 1170-1184.
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5

Characterization of Leukemic Cell Lines

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Human leukemic cell lines THP1, U937, K562, K562/A02, K562/G01, HL60, HL60/ADR cells and human embryonic kidney 293 T cells were purchased from the Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China. Murine leukemic cell line C1498 was purchased from ATCC. THP1, U937, K562, K562/A02, K562/G01 cells were cultured in RPMI 1640 medium, HL60 and HL60/ADR cells were cultured in IMDM medium, 293 T cells were cultured in DMEM medium (with 10% heat-inactivated fetal calf serum, Gibco; penicillin and streptomycin, Invitrogen; 37 °C, 5% CO2, in humidified incubator). Doxorubicin was added (final concentration of 0.5 μg/mL) to the complete culture medium of K562/A02 and HL60/ADR until 2 weeks before experiments. Multidrug resistant cell lines, HL60/ADR [26 (link)] and K562/A02 [27 (link)], and parental HL60 and K562 cell lines were used to investigate the effect of TNFAIP8 levels on chemoresistance in vitro. Cell line identity and purity were verified regularly by short tandem repeat profiling. The latest authentication of cell lines was conducted by Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (July 16 to August 23, 2019).
Pang Y., Zhao Y., Wang Y., Wang X., Wang R., Liu N., Li P., Ji M., Ye J., Sun T., Li J., Ma D., Lu F, & Ji C. (2020). TNFAIP8 promotes AML chemoresistance by activating ERK signaling pathway through interaction with Rac1. Journal of Experimental & Clinical Cancer Research : CR, 39, 158.
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6

Sourcing Cell Lines for Cancer Research

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4 T1, A20, B16F10, C1498, CT-26, E.G7-OVA, EL4, EMT-6, J558, JC, KLN205, L1210, L5178-R, LLC1, P388D1, RENCA and WEHI-3 were purchased from ATCC (Manassas, VA, USA). Colon 26 was purchased from RIKEN BioResource Research Center (Tsukuba, Ibaraki, Japan) and Nanjing CoBioer Biotechnology Co., Ltd. (Nanjing, China). H22 was purchased from China Center for Type Culture Collection (Beijing, China). LLC1-Luc was purchased from PerkinElmer (Waltham, MA, USA). MC38 and Panc02 were purchased from Obio Technology (Shanghai) Corp., Ltd. (Shanghai, China). RM-1 was purchased from Cell Bank of Shanghai Institutes for Biological Sciences of the Chinese Academy of Sciences (Shanghai, China). All cells were culture by following instructions of the providers.
Sato Y., Fu Y., Liu H., Lee M.Y, & Shaw M.H. (2021). Tumor-immune profiling of CT-26 and Colon 26 syngeneic mouse models reveals mechanism of anti-PD-1 response. BMC Cancer, 21, 1222.
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7

Murine AML Cell Line Characterization

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Murine AML cell line harboring a t(11;19) KMT2A-MLLT1 translocation (“MLL-ENL”), and primary murine AML harboring a t(11;19) KMT2A-MLLT3 translocation (“MLL-AF9”) were gifts from Deb DeRyckere. C1498 and Kasumi-1 were purchased from ATCC. Kasumi-1 cell identity was confirmed using short tandem repeat microsatellite loci analysis. Cell lines were maintained in RPMI medium plus 10% FBS and penicillin/streptomycin (cRPMI). MLL-AF9 cells were thawed and used immediately; they did not survive in vitro culture. For in vitro studies, MRX2843 was dissolved in dimethyl sulfoxide (DMSO; Sigma); vehicle controls were administered the equivalent volume of DMSO.
Cruz Cruz J., Allison K.C., Page L.S., Jenkins A.J., Wang X., Earp H.S., Frye S.V., Graham D.K., Verneris M.R, & Lee-Sherick A.B. (2023). Inhibiting efferocytosis reverses macrophage-mediated immunosuppression in the leukemia microenvironment. Frontiers in Immunology, 14, 1146721.
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8

Engineered Tumor Cell Lines for Immunotherapy

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C1498, E.G7-OVA, Hepa1-6, LLC1, and NCI-H446 were purchased from the ATCC. Colon38 was obtained from NIH (Bethesda, MD). GPC3 and/or chicken ovalbumin (OVA)-overexpressing Hepa1-6/ hGPC3 (29), LLC1/OVA/hGPC3, LLC1/hGPC3, and LLC1/OVA cells were established by transfecting GPC3 and/or OVA-expressing plasmids into parental cells to enhance the immunogenicity of each tumor, and also to allow us to test the combination of STA551 with a TRAB against GPC3. Human FcγRIIb-expressing CHOk1 cells (CHOk1/human FcγRIIb cells) were purchased from Promega, and CHO-DG44 from Thermo Fisher Scientific. Mouse FcγRIIboverexpressing CHO, human FcγRIIa-overexpressing CHO, and human FcγRIIb-overexpressing CHO were established by transfecting mouse FcγRIIb-expressing plasmids, H allotype of human FcγRIIaexpressing plasmids, or human FcγRIIb-expressing plasmids into parental CHO-DG44 cells. Human CD137-overexpressing CHO was established by transfecting human CD137-expressing plasmids into parental CHO-DG44 cells.
Kamata-Sakurai M., Narita Y., Hori Y., Nemoto T., Uchikawa R., Honda M., Hironiwa N., Taniguchi K., Shida-Kawazoe M., Metsugi S., Miyazaki T., Wada N.A., Ohte Y., Shimizu S., Mikami H., Tachibana T., Ono N., Adachi K., Sakiyama T., Matsushita T., Kadono S., Komatsu S.I., Sakamoto A., Horikawa S., Hirako A., Hamada K., Naoi S., Savory N., Satoh Y., Sato M., Noguchi Y., Shinozuka J., Kuroi H., Ito A., Wakabayashi T., Kamimura M., Isomura F., Tomii Y., Sawada N., Kato A., Ueda O., Nakanishi Y., Endo M., Jishage K.I., Kawabe Y., Kitazawa T, & Igawa T. (2021). Antibody to CD137 Activated by Extracellular Adenosine Triphosphate Is Tumor Selective and Broadly Effective In Vivo without Systemic Immune Activation. Cancer discovery, 11(1).
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9

Culturing Myeloid Cell Lines

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MonoMac6 (MM6) was obtained from DSMZ and cultured in RPMI1640 (11875119, Thermo Fisher Scientific) supplemented with 10% FBS plus 2 mM l-glutamine (25030-081, Thermo Fisher Scientific), 1× non-essential amino acids (11140050, Gibco), 1 mM sodium pyruvate (11360070, Thermo Fisher Scientific), 10 μg/mL human insulin (12585014, Thermo Fisher Scientific). THP1, U937, C1498, and Molm13 were purchased from ATCC and cultured in RPMI1640 supplemented with 10% FBS and 1% penicillin/streptomycin; HEK293T was purchased from ATCC and cultured in cultured in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin. All cells were maintained in a 37 °C, 5% CO2 humidified incubator. Plasmocin prophylactic was supplemented in all media to prevent potential contamination.
Zhang Z., Zhou K., Han L., Small A., Xue J., Huang H., Weng H., Su R., Tan B., Shen C., Li W., Zhao Z., Qing Y., Qin X., Wang K., Leung K., Boldin M., Chen C.W., Ann D., Qian Z., Deng X., Chen J, & Chen Z. (2023). RNA m6A reader YTHDF2 facilitates precursor miR-126 maturation to promote acute myeloid leukemia progression. Genes & Diseases, 11(1), 382-396.
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10

LILRB3 Signaling in Leukemia Cells

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THP-1, MV4–11, Molm13, U937, C1498 and 293T cells were purchased from ATCC. AML cell lines were cultured in RPMI-1640 with 10% FBS. Human anti-LILRB3 with the N297A mutation was coated onto the plate to activate LILRB3 signaling, and plates coated with human IgG (N297A) were used as controls. Cells infected with virus were cultured for at least an additional 3 weeks before analysis of LILRB3 signaling. Dead cells were identified using PI staining. Primary human T cells were isolated by autoMACS from donor PBMCs, stimulated with anti-CD3 and anti-CD28, and cultured in RPMI-1640 in the presence of IL-2. For the cytotoxic T lymphocyte assay, human AML cells were stained with CFSE and mixed at different ratios with activated T cells. After 10 hours, the percentage of PI-positive CFSE-stained AML cells was determined by FACS.
Wu G., Xu Y., Schultz R.D., Chen H., Xie J., Deng M., Liu X., Gui X., John S., Lu Z., Arase H., Zhang N., An Z, & Zhang C.C. (2021). LILRB3 supports acute myeloid leukemia development and regulates T-cell antitumor immune responses through the TRAF2–cFLIP–NF-κB signaling axis. Nature cancer, 2(11), 1170-1184.
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