Abbv 075

The BET protein degrader dBET6 was a gift from Nathanael Gray and previously described (Winter et al., 2017 (link)). dBET6 was used at a concentration of 100 nM in all experiments except those involving the iBRD4 system, in which it was used at 10 nM. The BET bromodomain inhibitor JQ1 was a gift from James Bradner and previously described (Filippakopoulos et al., 2010 (link)). JQ1 was used at a concentration of 500 nM for all experiments. The CDK9 inhibitor DRB (Cayman Chemical Company, 10010302) was used at a concentration of 100 μM for all experiments. The TFIIH inhibitor triptolide (EMD Millipore, 645900) was used at a concentration of 250 nM (HeLa) or 1 μM (HCT-116). The topoisomerase I inhibitor, camptothecin (Selleck Chemicals), was used at a concentration of 10 μM. The topoisomerase II inhibitor, dexrazoxane (Selleck Chemicals), was used at a concentration of 50 μM. The BET bromodomain inhibitors OTX015 (Selleck Chemicals), ABBV-075 (Selleck Chemicals), ABBV-744 (Selleck Chemicals), and PLX51107 (Selleck Chemicals) where used at 2 μM, 20 nM, 50 nM, and 2 μM, respectively.