α h3k4me3

Manufactured by Merck Group

Sourced in United States

α-H3K4me3 is a histone modification-specific antibody that recognizes the trimethylation of lysine 4 on histone H3. This antibody can be used for various applications in epigenetic research, such as chromatin immunoprecipitation (ChIP), western blotting, and immunofluorescence.

Automatically generated - may contain errors

Lab products found in correlation

α h3k9ac, by Merck Group (1 mentions) α h4k16ac, by Merck Group (1 mentions) Dynabeads protein a, by Thermo Fisher Scientific (1 mentions) α actin, by Merck Group (1 mentions) Epitect fast bisulfite kit, by Qiagen (1 mentions) Western lightning ecl kit, by PerkinElmer (1 mentions) Qiaamp dna mini kit, by Qiagen (1 mentions) α map2, by Merck Group (1 mentions) Mab377, by Merck Group (1 mentions) Dapi solution, by Thermo Fisher Scientific (1 mentions) α gfap, by Merck Group (1 mentions) α gfp, by Aves Labs (1 mentions) α h3k27ac, by Abcam (1 mentions)

Close spelling variants of this product

H3K4me3

4 protocols using α h3k4me3

Chromatin Immunoprecipitation Assays for Histone Modifications

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chromatin immunoprecipitation assays were carried out as described with modification [40 (link)]. Briefly, young, fully emerged BSMV-VIGS leaves with virus symptoms were inoculated with conidia of Bgt strain E09. After the indicated time, wheat leaves were fixed in 1% (v/v) formaldehyde and the nuclei were isolated and lysed. Cross-linked chromatin was sonicated and precleared with 50 µL of Dynabeads protein A (Invitrogen, Carlsbad, CA, USA) and then immunoprecipitated using antibodies α-TaHDT701, α-TaHDA6, α-TaHOS15, α-histone H4 (Millipore, Billerica, MA, USA), α-H4K16Ac (Millipore, Billerica, MA, USA), α-H3K9Ac (Millipore, Billerica, MA, USA), and α-H3K4me3 (Millipore, Billerica, MA, USA). DNA recovery after ChIP was quantified as the percentage of input. Relative enrichment of H4K16Ac, H3K9Ac, and H3K4me3 was calculated after normalizing the histone acetylation and methylation ChIP with histone H4 and H3 ChIP, respectively. Real-time PCRs were performed using gene-specific primers listed in Supplementary Table S1.

Zhi P., Kong L., Liu J., Zhang X., Wang X., Li H., Sun M., Li Y, & Chang C. (2020). Histone Deacetylase TaHDT701 Functions in TaHDA6-TaHOS15 Complex to Regulate Wheat Defense Responses to Blumeria graminis f.sp. tritici. International Journal of Molecular Sciences, 21(7), 2640.

+ Open protocol

+ Expand

Profiling Hippocampal Histone Marks

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blot analyses were carried out using Western Lightning ECL kit (Perkin-Elmer, Boston, MA), and α-H3K4me3 (Millipore 07-473) and α-actin (Sigma F5441) antibodies. DNA was extracted from adult hippocampi with QIAamp DNA Mini Kit (Qiagen). Bisulfite conversion of genomic DNA was performed with the EpiTect Fast bisulfite kit (Qiagen). Primer sequences for PCR amplification and pyrosequencing are provided in Supplemental Experimental Procedures.

Scandaglia M., Lopez-Atalaya J.P., Medrano-Fernandez A., Lopez-Cascales M.T., del Blanco B., Lipinski M., Benito E., Olivares R., Iwase S., Shi Y, & Barco A. (2017). Loss of Kdm5c causes spurious transcription and prevents the fine-tuning of activity-regulated enhancers in neurons. Cell reports, 21(1), 47-59.

+ Open protocol

+ Expand

Comprehensive Neuroanatomical Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nissl staining and immunostainings were performed as described (Ito et al., 2014 ). The primary antibodies used in this study are α-NeuN (Chemicon, MAB377), α-MAP2 (Sigma, M9942), α-GFAP (Sigma, G9269), α-DCX (Abcam, ab18723), α-Parvalbumin (Swant, PV235), α-Cre (gift from Schütz’s lab), α-H3K4me3 (Millipore 07-473), and α-GFP (Aves Labs, GFP-1020, valid also for recognizing EYFP). Nuclei were counterstained with a 1 nM DAPI solution (Invitrogen) before mounting. For magnetic resonance imaging (MRI), 4-month old Kdm5c-KO mice and wild-type littermates were examined.

+ Open protocol

+ Expand

ChIP-qPCR Analysis of Scn5a Locus

Check if the same lab product or an alternative is used in the 5 most similar protocols

ChIP assays were performed using control and denervated gastrocnemius samples from the same animal following the protocol described by Tarradas et al. [32 (link)]. Antibodies used for immunoprecipitation were α-H3K4me3 (07-473, Millipore, Burlington, MA, USA), α-H3K27ac (ab4729, Abcam, Cambridge, UK) and α-Gata4 (ma5-15532, Thermo Fisher). We analyzed the immunoprecipitated material by qPCR using primers specific for four regions of the rat Scn5a locus (Table S2). Results are shown as percentage of input.

Carreras D., Martinez-Moreno R., Pinsach-Abuin M., Santafe M.M., Gomà P., Brugada R., Scornik F.S., Pérez G.J, & Pagans S. (2021). Epigenetic Changes Governing Scn5a Expression in Denervated Skeletal Muscle. International Journal of Molecular Sciences, 22(5), 2755.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!