Human recombinant (rh−) BMP2 (355-BM) and activin A (338-AC) were from R&D Systems (Minneapolis, MN, USA). RQ1 RNase-Free DNase (M6101), random primers (C1181), MMLV-reverse transcriptase (M1701) and RNasin (N2511) were from Promega (Madison, WI, USA). SB431542 (S4317), pancreatin (P3292), and collagenase (Type I-C0130) were from Sigma (St. Louis, MO, USA). Media 199 (M199; 31100-035), Hanks’ Balanced Salt Solution (HBSS) without calcium/magnesium (14170-112), TRIzol Reagent, and SYBRgreen Supermix for qPCR were from Invitrogen (Burlington, ON, Canada). EvaGreen 2X qPCR MasterMix-S was from Applied Biological Materials Inc. (ABM, Richmond, BC, Canada). Oligonucleotides were purchased from IDT (Coralville, IA, USA). Gentamycin (450-135-XL), 100X antibiotic-antimycotic (450-115-EL) and deoxynucleotide triphosphates (dNTPs) were from Wisent (St-Bruno, Quebec, Canada). LDN 193189 hydrochloride (1509) was purchased from Axon MedChem (Reston, VA, USA) and compound C (171260) was from Calbiochem (EMD Chemicals Inc, Darmstadt, Germany).
Collagenase type 1 c0130
Manufactured by Merck Group
Sourced in United States
Collagenase (Type I-C0130) is an enzyme used for the digestion and dissociation of collagen-containing tissues. It is a naturally occurring enzyme derived from Clostridium histolyticum. The core function of Collagenase (Type I-C0130) is to break down the collagen matrix, which is a key structural component of various tissues.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using collagenase type 1 c0130
1
Osteogenic Differentiation Inducers
2
Xenograft Implantation of SINET Tumor Samples
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SINET surgical specimens were obtained from patients undergoing surgery at Sahlgrenska University Hospital, Gothenburg, Sweden. Tumours were xenografted to 6−15-week-old immunocompromised, non-obese severe combined immune-deficient interleukin-2 chain receptor γ knockout mice (NOG mice; Taconic) or NOG mice transgenic for human IL-2 (hIL2-NOG; Taconics). For subcutaneous transplantations, tumour tissue was cut into 1–2 mm2 pieces and either transplanted directly from surgery or after cryopreservation into the flank of the mouse. Orthotopic transplantations were performed with liver metastasis tumour pieces that had been incubated with 2 mg/mL collagenase type I (C-0130, Sigma-Aldrich) and >5 ng/mL deoxyribonuclease I (D-4263, Sigma-Aldrich) at 37 °C in 5% CO2 for 2 h, before being cryopreserved. Liver metastasis single-cell suspensions were injected into the mouse liver. When mice were sacrificed, autopsy was performed to validate any lack of tumour growth.
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