Ara 2 f nad

Manufactured by Biolog

Sourced in Germany

Ara-2"F-NAD+ is a chemical compound used in laboratory research. It is a synthetic analog of the coenzyme nicotinamide adenine dinucleotide (NAD+). This product is intended for use in scientific applications and experiments, but a detailed description of its core function cannot be provided without potentially making interpretations or extrapolations.

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Lab products found in correlation

Xestospongin c, by Santa Cruz Biotechnology (2 mentions) 8 br adpr, by Biolog (2 mentions) Rottlerin, by Bio-Techne (1 mentions) Go6976, by Bio-Techne (1 mentions) Skf96365, by Bio-Techne (1 mentions) Trans ned19, by Bio-Techne (1 mentions) Fla 7000, by Fujifilm (1 mentions)

4 protocols using ara 2 f nad

Inhibitors of Sarcoplasmic Reticulum Calcium Release

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Xestospongin C was obtained from Santa Cruz Biotechnology. Trans-Ned19, GF10923X, Go6976, rottlerin, and SKF96365 were obtained from Tocris Bioscience. 8-Br-ADPR and ara-2’-F-NAD were obtained from Biolog Life Science Institute. All other reagents were obtained from Sigma-Aldrich.

Rah S.Y., Joe Y., Park J., Ryter S.W., Park C., Chung H.T, & Kim U.H. (2023). CD38/ADP-ribose/TRPM2-mediated nuclear Ca2+ signaling is essential for hepatic gluconeogenesis in fasting and diabetes. Experimental & Molecular Medicine, 55(7), 1492-1505.

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Tpt1-Mediated Nucleic Acid Modification Assay

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Reaction mixtures containing 100 mM Tris-HCl (pH 7.5), 0.2 µM 5′ ³²P-labeled nucleic acid substrates, NAD⁺ or ara-2″F-NAD⁺ as specified, and Tpt1 as specified in the figure legends were incubated at 37°C. The reactions were quenched at the times specified in the figure legends by addition of 3 volumes of cold 90% formamide, 50 mM EDTA. The products were analyzed by electrophoresis (at 55 W constant power) through a 40-cm 20% polyacrylamide gel containing 7 M urea in 45 mM Tris-borate, 1 mM EDTA and visualized by autoradiography and/or scanning the gel with a Fujifilm FLA-7000 imaging device. The products were quantified by analysis of the gel scans in ImageQuant. Ara-2″F-NAD⁺ was purchased from BIOLOG (Bremen, Germany; Cat. No. D148).

Dantuluri S., Abdullahu L., Munir A., Katolik A., Damha M.J, & Shuman S. (2020). Substrate analogs that trap the 2′-phospho-ADP-ribosylated RNA intermediate of the Tpt1 (tRNA 2′-phosphotransferase) reaction pathway. RNA, 26(4), 373-381.

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Antibody Acquisition and Reagent Sourcing

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Antibodies were obtained as follows: granzyme B pAb from Cell Signaling (Danvers, MA) and perforin pAb from Santa Cruz Biotechnology (Santa Cruz, CA); anti-perforin mAb, DyLight 488 anti-perforin mAb, and DyLight 550 anti-TRPM2 pAb from Novus Biologicals (Littleton, CO). Human recombinant IL-2 was from Chiron BV (Amsterdam, Netherlands) and Xestospongin C was from Santa Cruz Biotechnology (Santa Cruz, CA). 8-Br-ADPR, ara-2′-F-NAD, and N-(p-amylcinnamoyl) anthranilic acid (ACA) were from Biolog Life Science Institute (Bremen, Germany). All other reagents were obtained from Sigma-Aldrich (St. Louis, MO).

Rah S.Y., Kwak J.Y., Chung Y.J, & Kim U.H. (2015). ADP-ribose/TRPM2-mediated Ca2+ signaling is essential for cytolytic degranulation and antitumor activity of natural killer cells. Scientific Reports, 5, 9482.

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Tpt1-Mediated NAD+ Analog Assay

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Reaction mixtures containing 100 mM Tris-HCl (pH 7.5), 0.2 µM 5′ ³²P-labeled nucleic acid substrates, NAD⁺ or NAD⁺ analogs as specified, and Tpt1 as specified in the figure legends were incubated at 37°C. The reactions were quenched at the times specified in the figure legends by addition of three volumes of cold 90% formamide, 50 mM EDTA. The products were analyzed by electrophoresis (at 55 W constant power) through a 40-cm 20% polyacrylamide gel containing 7 M urea in 45 mM Tris-borate, 1 mM EDTA and visualized by autoradiography and/or scanning the gel with a Fujifilm FLA-7000 imaging device. The products were quantified by analysis of the gel scans in ImageQuant. Ara-2″F-NAD⁺ was purchased from BIOLOG (Bremen, Germany; Cat. No. D148). NMN (nicotinamide mononucleotide), NAAD (nicotinic acid adenine dinucleotide), and NHD (nicotinamide hypoxanthine dinucleotide) were purchased from Sigma.

Dantuluri S., Schwer B., Abdullahu L., Damha M.J, & Shuman S. (2021). Activity and substrate specificity of Candida, Aspergillus, and Coccidioides Tpt1: essential tRNA splicing enzymes and potential antifungal targets. RNA, 27(5), 616-627.

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