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2 protocols using mouse monoclonal sb62a anti rabbit igg light chain hrp

1

Comprehensive Antibody Panel for Western Blotting and ChIP

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The following antibodies were used for western blotting: HDAC3 (GeneTex, GTX113303, Lot 40436), β–Actin [mAbcam 8226] (ABCAM, 20272), ERRα (ABCAM, 16363), HSP90 (Cell Signaling, 4874), Vinculin (Sigma, V9264), Acetylated Lysine Rabbit Polyclonal (Cell Signaling, 9441, Lot 12), UCP1 (R&D, MAB6158), HA Tag (GeneTex, 115044-01), Myc Tag (GeneTex, 21261), V5 Tag (ThermoFisher, R960-25), Pol II Antibody (N-20, Santa Cruz 899 X, Lot K1215), GCN5 (H-75, Santa Cruz 20698, Lot K0112), PGC-1α Mouse mAb (EMD Millipore, 4C.13, ST1202, Lot TE0349482), Anti-Rabbit IgG-HRP (Cell Signaling, 7074), Anti-mouse IgG-HRP (Cell Signaling, 7076), Mouse monoclonal SB62a Anti-Rabbit IgG light chain (HRP) (ABCAM, 99697). The following antibodies were used for ChIP: HDAC3 (ABCAM, 7030, Lot GR121157-6), H3K27ac (ABCAM, 4729, Lot GR251958-1), ERRα (ABCAM, 16363, Lot GR263385-1), NCoR (previously described39 , raised in rabbit against a.a.1944–2453, affinity purified), H3K4me1 (ABCAM 8895), and H3 (ABCAM 1791).
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2

Comprehensive Antibody Panel for Western Blotting and ChIP

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for western blotting: HDAC3 (GeneTex, GTX113303, Lot 40436), β–Actin [mAbcam 8226] (ABCAM, 20272), ERRα (ABCAM, 16363), HSP90 (Cell Signaling, 4874), Vinculin (Sigma, V9264), Acetylated Lysine Rabbit Polyclonal (Cell Signaling, 9441, Lot 12), UCP1 (R&D, MAB6158), HA Tag (GeneTex, 115044-01), Myc Tag (GeneTex, 21261), V5 Tag (ThermoFisher, R960-25), Pol II Antibody (N-20, Santa Cruz 899 X, Lot K1215), GCN5 (H-75, Santa Cruz 20698, Lot K0112), PGC-1α Mouse mAb (EMD Millipore, 4C.13, ST1202, Lot TE0349482), Anti-Rabbit IgG-HRP (Cell Signaling, 7074), Anti-mouse IgG-HRP (Cell Signaling, 7076), Mouse monoclonal SB62a Anti-Rabbit IgG light chain (HRP) (ABCAM, 99697). The following antibodies were used for ChIP: HDAC3 (ABCAM, 7030, Lot GR121157-6), H3K27ac (ABCAM, 4729, Lot GR251958-1), ERRα (ABCAM, 16363, Lot GR263385-1), NCoR (previously described39 , raised in rabbit against a.a.1944–2453, affinity purified), H3K4me1 (ABCAM 8895), and H3 (ABCAM 1791).
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